Plant regeneration from callus culture of Curcuma aromatica and in vitro detection of somaclonal variation through cytophotometric analysis
Open accessAbstract
Callus cultures initiated from shoot base explants of Curcuma aromatica Salisb. were maintained on Murashige and Skoog (MS) media supplemented with 2 mg dm-3 2,4-dichlorophenoxyacetic acid alone or with 0.5 mg dm-3 kinetin. Plantlets were regenerated from 60 and 180-d-old callus on MS media supplemented with 3 mg dm-3 benzyladenine and 0.5 mg dm-3 α-naphthalene acetic acid. Approximately 8-10 plantlets were produced after 30-40 d of culture per 50 mg of callus inoculated. Out of 113 regenerants analyzed 85 plants were exclusively diploid and 28 were predominantly diploid revealing presence of polyploid nuclei. Frequency of polyploid cells were more in regenerants obtained from 180-d-old callus then from 6-d-old callus which might be attributed to the ageing of callus.
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The age of the culture and the number of subcultures favor the appearance of somaclonal variations.
“The age of the culture and the number of subcultures favor the appearance of somaclonal variations.”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [26]
The passage labeled Abstract reports more polyploid cells in plants regenerated from 180-day-old callus than from 60-day-old callus and attributes this to callus aging. The copy does not establish that a greater number of subcultures favors variation; subculture frequency appears only in a cited work’s title in the References. No page numbers are provided for these passages. (LLM, conf=0.88)
Experimental — accuracy not yet measuredIn Curcuma aromatica, callus-regenerated plants were mostly exclusively diploid (75.22%), and only 24% were partially diploid; analysis confirmed diploid, tetraploid, and octoploid cells.
“Using flow-cytometry, Mohanty et al. (2008) [26] detected that the callus (initiated from shoots)-regenerated plants of Curcuma aromatica Salisb. were mostly exclusively diploid (75.22%), and only 24% of them were partially diploid; the analysis of the distribution of DNA content confirmed the presence of diploid, tetraploid, and octoploid cells.”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [26]
The copy’s Abstract reports 85 of 113 regenerants as exclusively diploid and 28 as predominantly diploid, with diploid, tetraploid, and octoploid cells detected in the latter group. Those counts yield 75.22% and 24.78%, not 24%. The methods passage describes cytophotometric analysis using a microspectrophotometer, not flow cytometry. (LLM, conf=0.91)
Experimental — accuracy not yet measuredIn Curcuma aromatica, the frequency of polyploid cells was higher in regenerants obtained from a 180-day-old callus compared to those from a 6-day-old callus.
“The frequency of polyploid cells was higher in the case of regenerants obtained from a 180-day-old callus compared to those in a 6-day-old callus.”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [26]
The paper reports a higher frequency of polyploid cells in regenerants from 180-day-old callus, but its detailed comparison is with 60-day-old callus (16–35% versus 1–6%). Although the Abstract says “6-d-old” once, it also identifies the studied callus ages as 60 and 180 days. The claim’s 6-day comparator is therefore not supported by the reported comparison. (LLM, conf=0.89)
Experimental — accuracy not yet measuredThe increased frequency of polyploid cells in Curcuma aromatica could be attributed to prolonged callus culture.
“So, the increased frequency of polyploid cells could be attributed to the prolonged callus culture [26].”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [26]
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Research works printed but not linked: 2
- Rout, G.R., Das, G.: In vitro studies of ginger: a review of recent progress. -In: Goril, J.N., Anandkumar, P., Singh, V.K. (ed.): Recent Progress in Medicinal Plants. Vol. 4. Pp. 307-326. Science Technology Publication, Studium Press, Houston 2002.
- Sharma, A.K., Sharma, A.: Chromosome Techniques: Theory & Practice. Third Edition. -Butterworths, London 1980.
Cited by
Sorted by citations
Somaclonal Variation in Plants: Causes and Detection Methods
Bairu, M. W., Aremu, A. O., & Van Staden, J. (2011) · Plant Growth Regulation · doi:10.1007/s10725-010-9554-x · Cited by 674 · see citing works
Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants
Duta-Cornescu, G., Constantin, N., Pojoga, D.-M., Nicuta, D., & Simon-Gruita, A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24010838 · Cited by 108 · see citing works
“(2008) [26] detected that the callus (initiated from shoots)-regenerated plants of Curcuma aromatica Salisb.”
BackgroundInfluentialSomaclonal Variation in Tissue Culture: A Case Study with Olive
Leva, A. R., Petruccelli, R., & Rinaldi, L. M. R. (2012) · Recent Advances in Plant in vitro Culture · doi:10.5772/50367 · Cited by 98 · see citing works
- Authors
- Mohanty, S., Panda, M. K., Subudhi, E., & Nayak, S.
- Affiliations
- Siksha O Anusandhan University (IN)
- Venue
- Biologia plantarum
- Publisher
- Institute of Experimental Botany
- Published
- 2008-12-01
- Volume
- 52
- Issue
- 4
- Pages
- 783-786
- ISSN
- 0006-3134 (ISSN-L)
- 1573-8264
- OpenAlex
- W1981085179
- Cited by
- 57
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 6 · 2023: 4 · 2022: 3 · 2021: 6 · 2020: 5 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 2 · 2013: 5 · 2012: 6
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biologia Plantarum (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Banana Cultivation and Research
- Keywords
- Curcuma aromatica, plant regeneration, callus culture, somaclonal variation, cytophotometry, polyploidy, benzyladenine, 2,4-D, kinetin, diploidy
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