Synthetic Microvascular Networks for Quantitative Analysis of Particle Adhesion
Open accessAbstract
We have developed a methodology to study particle adhesion in the microvascular environment using microfluidic, image-derived microvascular networks on a chip accompanied by Computational Fluid Dynamics (CFD) analysis of fluid flow and particle adhesion. Microfluidic networks, obtained from digitization of in vivo microvascular topology were prototyped using soft-lithography techniques to obtain semicircular cross sectional microvascular networks in polydimethylsiloxane (PDMS). Dye perfusion studies indicated the presence of well-perfused as well as stagnant regions in a given network. Furthermore, microparticle adhesion to antibody coated networks was found to be spatially non-uniform as well. These findings were broadly corroborated in the CFD analyses. Detailed information on shear rates and particle fluxes in the entire network, obtained from the CFD models, were used to show global adhesion trends to be qualitatively consistent with current knowledge obtained using flow chambers. However, in comparison with a flow chamber, this method represents and incorporates elements of size and complex morphology of the microvasculature. Particle adhesion was found to be significantly localized near the bifurcations in comparison with the straight sections over the entire network, an effect not observable with flow chambers. In addition, the microvascular network chips are resource effective by providing data on particle adhesion over physiologically relevant shear range from even a single experiment. The microfluidic microvascular networks developed in this study can be readily used to gain fundamental insights into the processes leading to particle adhesion in the microvasculature.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A vascularized microfluidic tumor model (MTM) consisting of a tumor compartment and a surrounding network of vessels, separated by a 2 μm wide porous interface, was developed and characterized.
“A vascularized MTM has been developed and characterized, consisting of a tumor compartment and a surrounding network of vessels, separated by a 2 μ m wide porous interface (Figure 1A,B). 17,21,26”
From Rapid Assessment of Nanoparticle Extravasation in a Microfluidic Tumor Model (2019) · cites this paper as [26]
The Introduction describes a microfluidic network of vessels developed to study particle adhesion, and Results §3.1 reports its fabrication and perfusion. The cited paper does not describe a tumor compartment or a 2 μm porous interface; Methods §2.2 instead describes a semicircular network sealed to glass. No page numbers are available in our copy. (LLM, conf=0.87)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Traffic signals for lymphocyte recirculation and leukocyte emigration: The multistep paradigm
Springer, T. A. (1994) · Cell · doi:10.1016/0092-8674(94)90337-9 · Cited by 7,009 · see citing works
White, F. M. (1974) · Cited by 5,753 · see citing works
Equation of motion for a small rigid sphere in a nonuniform flow
MAXEY, M. R., & Riley, J. J. (1983) · The Physics of Fluids · doi:10.1063/1.864230 · Cited by 3,476 · see citing works
Leukocyte-endothelial adhesion molecules
Carlos, T., & Harlan, J. (1994) · Blood · doi:10.1182/blood.v84.7.2068.bloodjournal8472068 · Cited by 2,500 · see citing works
El-Ali, J., Sorger, P. K., & Jensen, K. F. (2006) · Nature · doi:10.1038/nature05063 · Cited by 2,105 · see citing works
Nanomedicine: current status and future prospects
Moghimi, S. M., Hunter, A. C., & Murray, J. C. (2005) · The FASEB Journal · doi:10.1096/fj.04-2747rev · Cited by 2,029 · see citing works
Leukocyte-endothelial adhesion molecules
Carlos, T., & Harlan, J. (1994) · Blood · doi:10.1182/blood.v84.7.2068.2068 · Cited by 1,957 · see citing works
Anderson, J. R., Chiu, D. T.-Y., Jackman, R. J., et al. (2000) · Analytical Chemistry · doi:10.1021/ac9912294 · Cited by 698 · see citing works
Goldsmith, H. L., & Turitto, V. T. (1986) · PubMed · Cited by 476 · see citing works
Microfluidic Shear Devices for Quantitative Analysis of Cell Adhesion
Lu, H., Koo, L. Y., Wang, W. M., Lauffenburger, D. A., Griffith, L. G., & Jensen, K. F. (2004) · Analytical Chemistry · doi:10.1021/ac049837t · Cited by 406 · see citing works
Endothelial-Dependent Mechanisms of Leukocyte Recruitment to the Vascular Wall
Rao, R. M., Yang, L., García‐Cardeña, G., & Luscinskas, F. W. (2007) · Circulation Research · doi:10.1161/circresaha.107.151860b · Cited by 391 · see citing works
Rheological Aspects of Thrombosis and Haemostasis: Basic Principles and Applications
Goldsmith, H. L., & Turitto, V. T. (1986) · Thrombosis and Haemostasis · doi:10.1055/s-0038-1661576 · Cited by 391 · see citing works
Luscinskas, F. W., Kansas, G. S., Ding, H. T., Pizcueta, P. M., Schleiffenbaum, B. E., Tedder, T. F., & Gimbrone, M. A. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.125.6.1417 · Cited by 376 · see citing works
A dynamical model for receptor-mediated cell adhesion to surfaces
Hammer, D. A., & Lauffenburger, D. A. (1987) · Biophysical Journal · doi:10.1016/s0006-3495(87)83236-8 · Cited by 258 · see citing works
Functional heterogeneity of vascular endothelial cells
Gerritsen, M. E. (1987) · Biochemical Pharmacology · doi:10.1016/0006-2952(87)90252-8 · Cited by 257 · see citing works
The manufacture of microlenses by melting photoresist
Daly, D., Stevens, R. F., Hutley, M. C., et al. (1990) · Measurement Science and Technology · doi:10.1088/0957-0233/1/8/016 · Cited by 244 · see citing works
Particle Diameter Influences Adhesion under Flow
Patil, V. R. S., Campbell, C. J., Yun, Y., Slack, S. M., & Goetz, D. J. (2001) · Biophysical Journal · doi:10.1016/s0006-3495(01)76144-9 · Cited by 181 · see citing works
Sakhalkar, H. S., Dalal, M. K., Salem, A. K., Ansari, R., Fu, J., Kiani, M. F., Kurjiaka, D. T., Hanes, J. S., Shakesheff, K. M., & Goetz, D. J. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2631433100 · Cited by 174 · see citing works
Self-citationReceptor-mediated adhesion phenomena. Model studies with the Radical-Flow Detachment Assay
Cozens-Roberts, C., Quinn, J. A., & Lauffenberger, D. A. (1990) · Biophysical Journal · doi:10.1016/s0006-3495(90)82357-2 · Cited by 170 · see citing works
Vascular Targeting: Recent Advances and Therapeutic Perspectives
Hajitou, A., Pasqualini, R., & Arap, W. (2006) · Trends in Cardiovascular Medicine · doi:10.1016/j.tcm.2006.01.003 · Cited by 149 · see citing works
Integration of Technologies for Hepatic Tissue Engineering
Nahmias, Y., Francois Berthiaume, B., & Yarmush, M. L. (2006) · Springer eBooks · doi:10.1007/10_029 · Cited by 144 · see citing works
Isolated P-selectin Glycoprotein Ligand-1 Dynamic Adhesion to P- and E-selectin
Goetz, D. J., Greif, D. M., Ding, H. T., Camphausen, R. T., Howes, S., Comess, K. M., Snapp, K. R., Kansas, G. S., & Luscinskas, F. W. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.137.2.509 · Cited by 140 · see citing works
Targetability of novel immunoliposomes prepared by a new antibody conjugation technique
Bendas, G., Krause, A., Bakowsky, U., Vogel, J., & Rothe, U. (1999) · International Journal of Pharmaceutics · doi:10.1016/s0378-5173(99)00002-2 · Cited by 119 · see citing works
Late Effects of Ionizing Radiation on the Microvascular Networks in Normal Tissue
Nguyen, V., Gaber, M. W., Sontag, M. R., et al. (2000) · Radiation Research · doi:10.1667/0033-7587(2000)154[0531:leoiro]2.0.co;2 · Cited by 112 · see citing works
Adhesion molecules: a new target for immunoliposome‐mediated drug delivery
Bloemen, P. G. M., Henricks, P. A. J., van Bloois, L., et al. (1995) · FEBS Letters · doi:10.1016/0014-5793(94)01350-a · Cited by 111 · see citing works
Endothelial adhesion molecules in health and disease.
Cotran, R. S., & Mayadas‐Norton, T. (1998) · PubMed · Cited by 108 · see citing works
Radiation-induced intestinal inflammation
Mollà, M., & Panés, J. (2007) · World Journal of Gastroenterology · doi:10.3748/wjg.v13.i22.3043 · Cited by 97 · see citing works
Extravasation of Poly(amidoamine) (pamam) Dendrimers Across Microvascular Network Endothelium
ElSayed, M. E. H., Kiani, M. F., Naimark, M. D., et al. (2001) · Pharmaceutical Research · doi:10.1023/a:1011066408283 · Cited by 94 · see citing works
Self-citationEarly Effects of Ionizing Radiation on the Microvascular Networks in Normal Tissue
Roth, N. M., Sontag, M. R., & Kiani, M. F. (1999) · Radiation Research · doi:10.2307/3579938 · Cited by 92 · see citing works
Self-citationDynamics of neutrophil rolling over stimulated endothelium in vitro
Goetz, D. J., El-Sabban, M. E., Pauli, B. U., et al. (1994) · Biophysical Journal · doi:10.1016/s0006-3495(94)81016-1 · Cited by 70 · see citing works
Markers of Endothelial Dysfunction
Müller, M. M., & Griesmacher, A. (2000) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm.2000.013 · Cited by 63 · see citing works
Ligand Coated Nanosphere Adhesion to E- and P-Selectin under Static and Flow Conditions
Blackwell, J. E., Dagia, N. M., Dickerson, J. B., Berg, E. L., & Goetz, D. J. (2001) · Annals of Biomedical Engineering · doi:10.1114/1.1376697 · Cited by 62 · see citing works
Vascular endothelial cell adhesion and signaling during leukocyte recruitment.
Kluger, M. S. (2004) · PubMed · Cited by 61 · see citing works
Prabhakarpandian, B., Goetz, D. J., Swerlick, R. A., et al. (2001) · Microcirculation · doi:10.1111/j.1549-8719.2001.tb00182.x · Cited by 61 · see citing works
Self-citationYuan, H., Goetz, D. J., Gaber, M. W., et al. (2005) · Radiation Research · doi:10.1667/rr3361 · Cited by 61 · see citing works
Improvements to parallel plate flow chambers to reduce reagent and cellular requirements
Brown, D. C., & Larson, R. A. (2001) · BMC Immunology · doi:10.1186/1471-2172-2-9 · Cited by 57 · see citing works
Targeted delivery of antibody conjugated liposomal drug carriers to rat myocardial infarction
Scott, R. C., Wang, B., Nallamothu, R., et al. (2006) · Biotechnology and Bioengineering · doi:10.1002/bit.21233 · Cited by 55 · see citing works
Self-citationTargeting of the Antivascular Drug Combretastatin to Irradiated Tumors Results in Tumor Growth Delay
Pattillo, C. B., Sari‐Sarraf, F., Nallamothu, R., et al. (2005) · Pharmaceutical Research · doi:10.1007/s11095-005-5646-0 · Cited by 55 · see citing works
Patel, K. D. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.162.10.6209 · Cited by 54 · see citing works
Kiani, M. F., Yuan, H., Chen, X., Smith, L. A., Gaber, M. W., & Goetz, D. J. (2002) · Pharmaceutical Research · doi:10.1023/a:1020350708672 · Cited by 52 · see citing works
Self-citationFabrication of in Vitro Microvascular Blood Flow Systems by Photolithography
Cokelet, G. R., Soave, R. J., Pugh, G. O., et al. (1993) · Microvascular Research · doi:10.1006/mvre.1993.1062 · Cited by 49 · see citing works
Zou, X., Patil, V. R. S., Dagia, N. M., et al. (2005) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00289.2004 · Cited by 48 · see citing works
CD11b/CD18-coated microspheres attach to E-selectin under flow
Crutchfield, K. L., Patil, V. R. S., Campbell, C. J., Parkos, C. A., Allport, J. R., & Goetz, D. J. (2000) · Journal of Leukocyte Biology · doi:10.1002/jlb.67.2.196 · Cited by 41 · see citing works
Limited adhesion of biodegradable microspheres to E‐ and P‐selectin under flow
Dickerson, J. B., Blackwell, J. E., Ou, J. J., et al. (2001) · Biotechnology and Bioengineering · doi:10.1002/bit.1085 · Cited by 40 · see citing works
Burch, E. E., Patil, V. R. S., Camphausen, R. T., et al. (2002) · Blood · doi:10.1182/blood.v100.2.531 · Cited by 33 · see citing works
A System for Culture of Endothelial Cells in 20–50‐μm Branching Tubes
Frame, M. D. S., & Sarelius, I. H. (1995) · Microcirculation · doi:10.3109/10739689509148282 · Cited by 31 · see citing works
Vascular endothelium: recent advances.
Pasyk, K. A., & Jakobczak, B. A. (2004) · PubMed · Cited by 26 · see citing works
A “Geographic Information Systems” Based Technique for the Study of Microvascular Networks
Roth, N. M., & Kiani, M. F. (1999) · Annals of Biomedical Engineering · doi:10.1114/1.204 · Cited by 23 · see citing works
Self-citationLeukocyte Adhesion: An Exquisite Balance of Hydrodynamic and Molecular Forces
Tees, D. F. J., & Goetz, D. J. (2003) · Physiology · doi:10.1152/nips.01444.2003 · Cited by 19 · see citing works
Gaehtgens, P. A. L. (1992) · PubMed · Cited by 3 · see citing works
Flagged first, then by citations
Research works printed but not linked: 9
- Cotran RS Mayadas-Norton T Pathol. Biol. (Paris) 1998 46 164 70 9769911
- El-Sayed M Kiani MF Naimark MD Hikal AH Ghandehari H Pharm. Res 2001 18 23 8 11336349
- Gaehtgens P Int. J. Microcirc. Clin. Exp 1992 11 123 132 1612826
- Goldsmith HL Turitto VT Thromb. Haemost 1986 55 415 35 3750272
- Kluger MS Adv. Dermatol 2004 20 163 201 15544200
- Nguyen V Gaber MW Sontag MR Kiani MF Radiat. Res 2000 154 531 536 11025649
- Pasyk KA Jakobczak BA Eur J Dermatol 2004 14 209 13 15319151
- Patillo CB Sari-Sarraf F Nallamothu R Moore BM Wood GC Kiani MF Pharm. Res 2005 22 1117 1120 16028012
- Roth NM Sontag MR Kiani MF Radiat. Res 1999 151 270 277 10073664
Cited by
Sorted by citations
Microfluidic 3D models of cancer
Sung, K. E., & Beebe, D. J. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.07.002 · Cited by 190 · see citing works
Tang, Y., Soroush, F., Sheffield, J. B., Wang, B., Prabhakarpandian, B., & Kiani, M. F. (2017) · Scientific Reports · doi:10.1038/s41598-017-09815-9 · Cited by 102 · see citing works
Self-citationSynthetic tumor networks for screening drug delivery systems
Prabhakarpandian, B., Shen, M.-C., Nichols, J. B., Garson, C. J., Mills, I. R., Matar, M. M., Fewell, J. G., & Pant, K. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.01.018 · Cited by 57 · see citing works
Self-citationRapid Assessment of Nanoparticle Extravasation in a Microfluidic Tumor Model
Vu, M. N., Rajasekhar, P., Poole, D. P., Khor, S. Y., Truong, N. P., Nowell, C. J., Quinn, J. F., Whittaker, M., Veldhuis, N. A., & Davis, T. P. (2019) · ACS Applied Nano Materials · doi:10.1021/acsanm.8b02056 · Cited by 52 · see citing works
- Authors
- Prabhakarpandian, B., Pant, K., Scott, R. C., Patillo, C. B., Irimia, D., Kiani, M. F., & Sundaram, S.
- Affiliations
- CFD Research Corporation (United States) (US)
- Temple University (US)
- Massachusetts General Hospital (US)
- Corresponding author
- Sundaram, Shivshankar
- Venue
- Biomedical Microdevices
- Published
- 2008-03-08
- Volume
- 10
- Issue
- 4
- Pages
- 585-595
- ISSN
- 1387-2176 (ISSN-L)
- 1572-8781
- PMC
- PMC4476637
- OpenAlex
- W2079340368
- Cited by
- 68
- References
- 50
- Citations by year
- 2024: 2 · 2023: 1 · 2021: 5 · 2020: 6 · 2019: 3 · 2018: 8 · 2017: 5 · 2016: 3 · 2015: 5 · 2014: 6 · 2013: 3 · 2012: 5
- FWCI
- 3.54
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Biomedical Microdevices (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R44HL076034)
- National Institute of Biomedical Imaging and Bioengineering (P41EB002503)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Blood properties and coagulation
- Keywords
- particle adhesion, microvascular networks, microfluidic networks, computational fluid dynamics, shear rate, particle flux, stagnant regions, polydimethylsiloxane, soft lithography, flow chamber, microvascular blood flow
- MeSH terms
- Adhesiveness, Equipment Design, Humans, Immunoglobulin G, Microcirculation, Microspheres, Models, Theoretical, Particle Size, P-Selectin, Coated Materials, Biocompatible, Microfluidic Analytical Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
