Recruitment of Plasma Membrane GABA-A Receptors by Submembranous Gephyrin/Collybistin Clusters

George, Shanu; Chiou, Tzu-Ting; Kanamalla, Karthik; De Blas, Angel L.

doi:10.1007/s10571-021-01050-1

Cited by 8Neuroscience and Neuropharmacology ResearchNicotinic Acetylcholine Receptors StudyReceptor Mechanisms and Signaling

Recruitment of Plasma Membrane GABA-A Receptors by Submembranous Gephyrin/Collybistin Clusters

Open access

George, Shanu, Chiou, Tzu-Ting, Kanamalla, Karthik, De Blas, Angel L.·2022·Cellular and Molecular Neurobiology

DOI 10.1007/s10571-021-01050-1PMC PMC11421751

Open this paper in CoChat — free account

Abstract

It has been shown that subunit composition is the main determinant of the synaptic or extrasynaptic localization of GABA A receptors (GABA A Rs). Synaptic and extrasynaptic GABA A Rs are involved in phasic and tonic inhibition, respectively. It has been proposed that synaptic GABA A Rs bind to the postsynaptic gephyrin/collybistin (Geph/CB) lattice, but not the typically extrasynaptic GABA A Rs. Nevertheless, there are no studies of the direct binding of various types of GABA A Rs with the submembranous Geph/CB lattice in the absence of other synaptic proteins, some of which are known to interact with GABA A Rs. We have reconstituted GABA A Rs of various subunit compositions, together with the Geph/CB scaffold, in HEK293 cells, and have investigated the recruitment of surface GABA A Rs by submembranous Geph/CB clusters. Results show that the typically synaptic α1β3γ2 GABA A Rs were trapped by submembranous Geph/CB clusters. The α5β3γ2 GABA A Rs, which are both synaptic and extrasynaptic, were also trapped by Geph/CB clusters. Extrasynaptic α4β3δ GABA A Rs consistently showed little or no trapping by the Geph/CB clusters. However, the extrasynaptic α6β3δ, α1β3, α6β3 (and less α4β3) GABA A Rs were highly trapped by the Geph/CB clusters. AMPA and NMDA glutamate receptors were not trapped. The results suggest: (I) in the absence of other synaptic molecules, the Geph/CB lattice has the capacity to trap not only synaptic but also several typically extrasynaptic GABA A Rs; (II) the…

Integrity signals

Registry

Crossref · 10.1007/s10571-021-01050-1

Cites

144 of 144 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Experimental reconstitution studies in HEK293 cells have demonstrated that the gephyrin/CB lattice can directly trap various GABAAR subunit compositions, including typically synaptic α1β3γ2 GABAARs and some extrasynaptic subtypes like α6β3δ and α1β3.
“Experimental reconstitution studies in HEK293 cells have demonstrated that the gephyrin/CB lattice can directly trap various GABAAR subunit compositions, including typically synaptic α1β3γ2 GABAARs and some extrasynaptic subtypes like α6β3δ and α1β3.”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
Gephyrin is essential for the clustering and anchoring of a large proportion of synaptic GABAARs, though not all.
“Gephyrin is essential for the clustering and anchoring of a large proportion of synaptic GABAARs, though not all.”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
GABAARs are initially delivered to the cell surface at non-synaptic sites via exocytosis and then undergo lateral diffusion in the plasma membrane to be trapped at the synapse.
“GABAARs are initially delivered to the cell surface at non-synaptic sites via exocytosis and then undergo lateral diffusion in the plasma membrane to be trapped at the synapse.”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
Other extrasynaptic receptors, such as α4β3δ, show little or no trapping, suggesting that additional neuronal mechanisms prevent the trapping of certain extrasynaptic GABAARs by the gephyrin/CB lattice in neurons.
“However, other extrasynaptic receptors, such as α4β3δ, show little or no trapping, suggesting that additional neuronal mechanisms are at play to prevent the trapping of certain extrasynaptic GABAARs by the gephyrin/CB lattice in neurons [57].”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
Trapping of GABAARs at the synapse is thought to occur through the interaction of the large intracellular loop of the α subunits of synaptic GABAARs with the postsynaptic Gephyrin/CB lattice.
“This trapping is thought to occur through the interaction of the large intracellular loop (IL) of the α subunits of synaptic GABAARs with the postsynaptic Gephyrin/CB lattice.”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]

The intracellular loop of the α2 GABAAR subunit not only binds to gephyrin but also activates collybistin, which is important for targeting α2 GABAARs to the axon initial segment.
“Notably, the IL of the α2 GABAAR subunit not only binds to gephyrin but also activates collybistin, which is important for targeting α2 GABAARs to the axon initial segment.”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
Gephyrin post-translational modifications modulate its affinity for GABAergic and glycinergic receptors, thus balancing inhibitory stability with plastic remodeling.
“These modulate its affinity for GABAergic and glycinergic receptors, thus balancing inhibitory stability with plastic remodeling [57,58,59,60].”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
Gephyrin is a multifunctional scaffold protein that forms submembranous postsynaptic planar lattices at both GABAergic and glycinergic synapses.
“Gephyrin is a multifunctional scaffold protein that forms submembranous postsynaptic planar lattices at both GABAergic and glycinergic synapses [57].”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
The gephyrin/CB scaffold is crucial but not the sole determinant for GABAAR synaptic localization; other synaptic molecules and post-translational modifications also contribute.
“This indicates that while the gephyrin/CB scaffold is crucial, it is not the sole determinant for GABAAR synaptic localization, and other synaptic molecules (e.g., GARLH4, neuroligin 2) and post-translational modifications (e.g., phosphorylation of gephyrin and GABAAR subunit ILs) also contribute to the complex regulation of GABAAR clustering and synaptic localization [57].”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
The β3 subunit appears to be a major determinant for the interaction of GABAARs with gephyrin/CB clusters.
“The β3 subunit appears to be a major determinant for the interaction of GABAARs with gephyrin/CB clusters [57].”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
The intracellular loops of α1, α2, α3, or α5 subunits bind to monomeric gephyrin in vitro and to collybistin, playing a critical role in their synaptic localization.
“Studies have shown that the ILs of α1, α2, α3, or α5 subunits bind to monomeric gephyrin in vitro and to collybistin, playing a critical role in their synaptic localization [57].”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
Collybistin acts as a guanine nucleotide exchange factor for Cdc42, and its deficiency leads to disrupted postsynaptic clustering of GABAARs and gephyrin in various brain regions, including hippocampus, amygdala, and cerebellum.
“Collybistin acts as a guanine nucleotide exchange factor (GEF) for the small GTPase Cdc42, and its deficiency leads to disrupted postsynaptic clustering of GABAARs and Gephyrin in various brain regions, including the hippocampus, amygdala, and cerebellum [57].”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]
Collybistin binds to gephyrin and membrane phosphoinositides, thereby anchoring the gephyrin/CB lattice to the postsynaptic membrane.
“The postsynaptic scaffold also includes collybistin (CB), which binds to gephyrin and membrane phosphoinositides, thereby anchoring the gephyrin/CB lattice to the postsynaptic membrane [57].”
From Molecular Physiology of the Neuronal Synapse (2026) · cites this paper as [57]

Epigenetic Regulations of GABAergic Neurotransmission: Relevance for Neurological Disorders and Epigenetic Therapy
Shrestha, S., & Offer, S. M. (2016) · Medical Epigenetics · doi:10.1159/000444713 · Cited by 8,949 · see citing works
GABAA receptors: immunocytochemical distribution of 13 subunits in the adult rat brain
Pirker, S., Schwarzer, C., Wieselthaler, A., Sieghart, W., & Sperk, G. (2000) · Neuroscience · doi:10.1016/s0306-4522(00)00442-5 · Cited by 1,344 · see citing works
International Union of Pharmacology. LXX. Subtypes of γ-Aminobutyric AcidA Receptors: Classification on the Basis of Subunit Composition, Pharmacology, and Function. Update
Olsen, R. W., & Sieghart, W. (2008) · Pharmacological Reviews · doi:10.1124/pr.108.00505 · Cited by 1,064 · see citing works
Subunit Composition, Distribution and Function of GABA-A Receptor Subtypes
Sieghart, W., & Sperk, G. (2002) · Current Topics in Medicinal Chemistry · doi:10.2174/1568026023393507 · Cited by 914 · see citing works
Postsynaptic clustering of major GABAA receptor subtypes requires the γ2 subunit and gephyrin
Essrich, C., Lorez, M., Benson, J. A., Fritschy, J., & Lüscher, B. (1998) · Nature Neuroscience · doi:10.1038/2798 · Cited by 856 · see citing works
Segregation of Different GABAAReceptors to Synaptic and Extrasynaptic Membranes of Cerebellar Granule Cells
Nusser, Z., Sieghart, W., & Somogyi, P. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-05-01693.1998 · Cited by 823 · see citing works
GABAA receptor trafficking and its role in the dynamic modulation of neuronal inhibition
Jacob, T. C., Moss, S. J., & Jurd, R. (2008) · Nature Reviews Neuroscience · doi:10.1038/nrn2370 · Cited by 695 · see citing works
Extrasynaptic GABAA Receptors: Their Function in the CNS and Implications for Disease
Brickley, S. G., & Módy, I. (2012) · Neuron · doi:10.1016/j.neuron.2011.12.012 · Cited by 674 · see citing works
GABAA receptor signalling mechanisms revealed by structural pharmacology
Masiulis, S., Desai, R., Uchański, T., Martin, I. S., Laverty, D. C., Karia, D., Malinauskas, T., Zivanov, J., Pardon, E., Kotecha, A., et al. (2018) · Nature · doi:10.1038/s41586-018-0832-5 · Cited by 559 · see citing works
Neuroligin 2 is exclusively localized to inhibitory synapses
Varoqueaux, F., Jamain, S., & Brose, N. (2004) · European Journal of Cell Biology · doi:10.1078/0171-9335-00410 · Cited by 552 · see citing works
Trace fear conditioning involves hippocampal α 5 GABA A receptors
Crestani, F., Keist, R., Fritschy, J.-M., Benke, D., Vogt, K., Prut, L., Blüthmann, H., Möhler, H., & Rudolph, U. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.142288699 · Cited by 528 · see citing works
The Dynamic Synapse
Choquet, D., & Triller, A. (2013) · Neuron · doi:10.1016/j.neuron.2013.10.013 · Cited by 515 · see citing works
Neuroligin 2 Drives Postsynaptic Assembly at Perisomatic Inhibitory Synapses through Gephyrin and Collybistin
Poulopoulos, A., Aramuni, G., Meyer, G., Soykan, T., Hoon, M., Papadopoulos, T. G., Zhang, M., Paarmann, I., Fuchs, C., Harvey, K., et al. (2009) · Neuron · doi:10.1016/j.neuron.2009.08.023 · Cited by 503 · see citing works
Stoichiometry and Assembly of a Recombinant GABAAReceptor Subtype
Tretter, V. E., Ehya, N., Fuchs, K., & Sieghart, W. (1997) · Journal of Neuroscience · doi:10.1523/jneurosci.17-08-02728.1997 · Cited by 484 · see citing works
Loss of Postsynaptic GABAA Receptor Clustering in Gephyrin-Deficient Mice
Kneussel, M., Brandstätter, J., Laube, B., Stahl, S., Müller, U., & Betz, H. (1999) · Journal of Neuroscience · doi:10.1523/jneurosci.19-21-09289.1999 · Cited by 456 · see citing works
GABAA Receptor Trafficking-Mediated Plasticity of Inhibitory Synapses
Lüscher, B., Fuchs, T. A. E., & Kilpatrick, C. L. (2011) · Neuron · doi:10.1016/j.neuron.2011.03.024 · Cited by 432 · see citing works
Gephyrin: a master regulator of neuronal function?
Tyagarajan, S. K., & Fritschy, J. (2014) · Nature reviews. Neuroscience · doi:10.1038/nrn3670 · Cited by 417 · see citing works
Perisynaptic Localization of δ Subunit-Containing GABAAReceptors and Their Activation by GABA Spillover in the Mouse Dentate Gyrus
Wei, W., Zhang, N., Peng, Z., Houser, C. R., & Módy, I. (2003) · Journal of Neuroscience · doi:10.1523/jneurosci.23-33-10650.2003 · Cited by 415 · see citing works
Identification of a gephyrin binding motif on the glycine receptor β subunit
Meyer, G., Kirsch, J., Betz, H., & Langosch, D. (1995) · Neuron · doi:10.1016/0896-6273(95)90145-0 · Cited by 406 · see citing works
Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts
Loh, K. H., Stawski, P. S., Draycott, A. S., Udeshi, N. D., Lehrman, E. K., Wilton, D. K., Svinkina, T., Deerinck, T. J., Ellisman, M. H., Stevens, B., et al. (2016) · Cell · doi:10.1016/j.cell.2016.07.041 · Cited by 399 · see citing works
Identification of an elaborate complex mediating postsynaptic inhibition
Uezu, A., Kanak, D. J., Bradshaw, T. W. A., Soderblom, E. J., Catavero, C. M., Burette, A. C., Weinberg, R. J., & Soderling, S. H. (2016) · Science · doi:10.1126/science.aag0821 · Cited by 354 · see citing works
Assembly and Cell Surface Expression of Heteromeric and Homomeric γ-Aminobutyric Acid Type A Receptors
Connolly, C. N., Krishek, B. J., McDonald, B. J., Smart, T. G., & Moss, S. J. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.1.89 · Cited by 333 · see citing works
Cryo-EM structure of the human α1β3γ2 GABAA receptor in a lipid bilayer
Laverty, D., Desai, R., Uchański, T., Masiulis, S., Stec, W. J., Malinauskas, T., Zivanov, J., Pardon, E., Steyaert, J., Miller, K. W., et al. (2019) · Nature · doi:10.1038/s41586-018-0833-4 · Cited by 316 · see citing works
GABA A receptor α4 subunits mediate extrasynaptic inhibition in thalamus and dentate gyrus and the action of gaboxadol
Chandra, D., Jia, F., Liang, J., Peng, Z., Suryanarayanan, A., Werner, D. F., Spigelman, I., Houser, C. R., Olsen, R. W., Harrison, N. L., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604304103 · Cited by 315 · see citing works
Constitutive Endocytosis of GABAAReceptors by an Association with the Adaptin AP2 Complex Modulates Inhibitory Synaptic Currents in Hippocampal Neurons
Kittler, J. T., Delmas, P., Jovanovic, J. N., Brown, D. A., Smart, T. G., & Moss, S. J. (2000) · Journal of Neuroscience · doi:10.1523/jneurosci.20-21-07972.2000 · Cited by 314 · see citing works
Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein
Prior, P. P., Schmitt, B., Grenningloh, G., Pribilla, I., Multhaup, G., Beyreuther, K. T., Maulet, Y., Werner, P., Langosch, D., Kirsch, J., et al. (1992) · Neuron · doi:10.1016/0896-6273(92)90136-2 · Cited by 313 · see citing works
The 93-kDa glycine receptor-associated protein binds to tubulin.
Kirsch, J., Langosch, D., Prior, P., Littauer, U., Schmitt, B., & Betz, H. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54560-9 · Cited by 284 · see citing works
Collybistin, a newly identified brain-specific GEF, induces submembrane clustering of gephyrin
Kins, S., Betz, H., & Kirsch, J. (2000) · Nature Neuroscience · doi:10.1038/71096 · Cited by 281 · see citing works
Intact sorting, targeting, and clustering of γ‐aminobutyric acid A receptor subtypes in hippocampal neurons in vitro
Brünig, I., Scotti, E., Sidler, C., & Fritschy, J. (2001) · The Journal of Comparative Neurology · doi:10.1002/cne.10102 · Cited by 278 · see citing works
The GDP-GTP Exchange Factor Collybistin: An Essential Determinant of Neuronal Gephyrin Clustering
Harvey, K., Duguid, I., Alldred, M. J., Beatty, S. E., Ward, H., Keep, N., Lingenfelter, S., Pearce, B., Lundgren, J., Owen, M., et al. (2004) · Journal of Neuroscience · doi:10.1523/jneurosci.1184-04.2004 · Cited by 270 · see citing works
Stoichiometry of a Ligand-gated Ion Channel Determined by Fluorescence Energy Transfer
Farrar, S. J., Whiting, P. J., Bonnert, T. P., & McKernan, R. M. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.15.10100 · Cited by 269 · see citing works
The γ2 subunit of GABAA receptors is required for maintenance of receptors at mature synapses
Schweizer, C. (2003) · Molecular and Cellular Neuroscience · doi:10.1016/s1044-7431(03)00202-1 · Cited by 264 · see citing works
The Clustering of GABAA Receptor Subtypes at Inhibitory Synapses is Facilitated via the Direct Binding of Receptor α2 Subunits to Gephyrin
Tretter, V., Jacob, T. C., Mukherjee, J., Fritschy, J., Pangalos, M., & Moss, S. (2008) · Journal of Neuroscience · doi:10.1523/jneurosci.5050-07.2008 · Cited by 260 · see citing works
Gephyrin Is Critical for Glycine Receptor Clustering But Not for the Formation of Functional GABAergic Synapses in Hippocampal Neurons
Lévi, S., Logan, S. M., Tovar, K., & Craig, A. M. (2004) · Journal of Neuroscience · doi:10.1523/jneurosci.1661-03.2004 · Cited by 246 · see citing works
GABA Potency at GABAA Receptors Found in Synaptic and Extrasynaptic Zones
Mortensen, M., Patel, B., & Smart, T. G. (2012) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2012.00001 · Cited by 244 · see citing works
Single-Channel Properties of Synaptic and Extrasynaptic GABAAReceptors Suggest Differential Targeting of Receptor Subtypes
Brickley, S. G., Cull-Candy, S. G., & Farrant, M. (1999) · Journal of Neuroscience · doi:10.1523/jneurosci.19-08-02960.1999 · Cited by 236 · see citing works
Assembly of GABAA receptor subunits: alpha 1 beta 1 and alpha 1 beta 1 gamma 2S subunits produce unique ion channels with dissimilar single- channel properties
Angelotti, T., & Macdonald, R. L. (1993) · Journal of Neuroscience · doi:10.1523/jneurosci.13-04-01429.1993 · Cited by 232 · see citing works
Subunit Composition and Quantitative Importance of Hetero-oligomeric Receptors: GABAAReceptors Containing α6Subunits
Jechlinger, M., Pelz, R., Tretter, V. E., Klausberger, T., & Sieghart, W. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-07-02449.1998 · Cited by 201 · see citing works
Impaired GABAergic transmission and altered hippocampal synaptic plasticity in collybistin‐deficient mice
Papadopoulos, T. G., Körte, M., Eulenburg, V., Kubota, H., Retiounskaia, M., Harvey, R. J., Harvey, K., O’Sullivan, G. A., Laube, B., Hülsmann, S., et al. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601819 · Cited by 195 · see citing works
Cell Surface Stability of γ-Aminobutyric Acid Type A Receptors
Connolly, C. N., Kittler, J. T., Thomas, P., Uren, J. M., Brandon, N. J., Smart, T. G., & Moss, S. J. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.51.36565 · Cited by 185 · see citing works
Gephyrin-independent clustering of postsynaptic GABA(A) receptor subtypes.
Kneussel, M., Brandstätter, J., Gasnier, B., Feng, G., Sanes, J., & Betz, H. (2001) · Molecular and cellular neurosciences · doi:10.1006/mcne.2001.0983 · Cited by 179 · see citing works
Extrasynaptic αβ subunit GABA A receptors on rat hippocampal pyramidal neurons
Mortensen, M., & Smart, T. G. (2006) · The Journal of Physiology · doi:10.1113/jphysiol.2006.117952 · Cited by 167 · see citing works
Synaptic and nonsynaptic localization of GABAAreceptors containing the α5 subunit in the rat brain
Serwanski, D. R., Miralles, C. P., Christie, S. B., Mehta, A. K., Li, X., & Blas, A. L. D. (2006) · The Journal of Comparative Neurology · doi:10.1002/cne.21115 · Cited by 164 · see citing works
Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex
de Blas, A., Vitórica, J., & Friedrich, P. (1988) · Journal of Neuroscience · doi:10.1523/jneurosci.08-02-00602.1988 · Cited by 161 · see citing works
The Residence Time of GABAARs at Inhibitory Synapses Is Determined by Direct Binding of the Receptor α1 Subunit to Gephyrin
Mukherjee, J. T., Kretschmannova, K., Gouzer, G., Maric, H. M., Ramsden, S. L., Tretter, V. E., Harvey, K., Davies, P. A., Triller, A., Schindelin, H., et al. (2011) · Journal of Neuroscience · doi:10.1523/jneurosci.2001-11.2011 · Cited by 160 · see citing works
NMDA receptors regulate GABA A receptor lateral mobility and clustering at inhibitory synapses through serine 327 on the γ2 subunit
Muir, J., Arancibia-Carcamo, I. L., MacAskill, A. F., Smith, K. R., Griffin, L. D., & Kittler, J. T. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1000589107 · Cited by 158 · see citing works
Gephyrin: a central GABAergic synapse organizer
Choii, G., & Ko, J. (2015) · Experimental & Molecular Medicine · doi:10.1038/emm.2015.5 · Cited by 157 · see citing works
Distinct γ2 Subunit Domains Mediate Clustering and Synaptic Function of Postsynaptic GABAAReceptors and Gephyrin
Alldred, M. J., Mulder-Rosi, J., Lingenfelter, S. E., Chen, G., & Lüscher, B. (2005) · Journal of Neuroscience · doi:10.1523/jneurosci.4011-04.2005 · Cited by 152 · see citing works
Quantitative localisation of synaptic and extrasynaptic GABAAreceptor subunits on hippocampal pyramidal cells by freeze‐fracture replica immunolabelling
Kasugai, Y., Swinny, J. D., Roberts, J. D. B., Dalezios, Y., Fukazawa, Y., Sieghart, W., Shigemoto, R., & Somogyi, P. (2010) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2010.07473.x · Cited by 149 · see citing works
Genetic and Molecular Regulation of Extrasynaptic GABA-A Receptors in the Brain: Therapeutic Insights for Epilepsy
Chuang, S.-H., & Reddy, D. S. (2017) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.117.244673 · Cited by 149 · see citing works
Subcellular localization of gamma-aminobutyric acid type A receptors is determined by receptor beta subunits.
Connolly, C. N., Wooltorton, J. R. A., Smart, T. G., & Moss, S. J. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.18.9899 · Cited by 143 · see citing works
Targeting of glycine receptor subunits to gephyrin-rich domains in transfected human embryonic kidney cells.
Kirsch, J., Kuhse, J., & Betz, H. (1995) · Molecular and cellular neurosciences · doi:10.1006/mcne.1995.1033 · Cited by 139 · see citing works
Radixin regulates synaptic GABAA receptor density and is essential for reversal learning and short-term memory
Hausrat, T. J., Muhia, M., Gerrow, K., Thomas, P., Hirdes, W., Tsukita, S., Heisler, F. F., Herich, L., Dubroqua, S., Breiden, P., et al. (2015) · Nature Communications · doi:10.1038/ncomms7872 · Cited by 138 · see citing works
A Balanced Chromosomal Translocation Disrupting ARHGEF9 Is Associated With Epilepsy, Anxiety, Aggression, and Mental Retardation
Kalscheuer, V., Musante, L., Fang, C., Hoffmann, K., Fuchs, C., Carta, E., Deas, E., Venkateswarlu, K., Menzel, C., Ullmann, R., et al. (2009) · Human Mutation · doi:10.1002/humu.20814 · Cited by 138 · see citing works
Complex Role of Collybistin and Gephyrin in GABAA Receptor Clustering*
Saiepour, L., Fuchs, C., Patrizi, A., Sassoè-Pognetto, M., Harvey, R., & Harvey, K. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.121368 · Cited by 137 · see citing works
Distinct mechanisms regulate GABAA receptor and gephyrin clustering at perisomatic and axo‐axonic synapses on CA1 pyramidal cells
Panzanelli, P., Gunn, B. G., Schlatter, M. C., Benke, D., Tyagarajan, S. K., Scheiffele, P., Belelli, D., Lambert, J. J., Rudolph, U., & Fritschy, J. (2011) · The Journal of Physiology · doi:10.1113/jphysiol.2011.216028 · Cited by 136 · see citing works
GARLH Family Proteins Stabilize GABAA Receptors at Synapses
Yamasaki, T., Hoyos, E., Martenson, J. S., Morimoto‐Tomita, M., & Tomita, S. (2017) · Neuron · doi:10.1016/j.neuron.2017.02.023 · Cited by 133 · see citing works
Complex Formation between the Postsynaptic Scaffolding Protein Gephyrin, Profilin, and Mena: A Possible Link to the Microfilament System
Giesemann, T., Schwarz, G., Nawrotzki, R., Berhörster, K., Rothkegel, M., Schlüter, K., Schrader, N., Schindelin, H., Mendel, R., Kirsch, J., et al. (2003) · Journal of Neuroscience · doi:10.1523/jneurosci.23-23-08330.2003 · Cited by 131 · see citing works
Gephyrin, the enigmatic organizer at GABAergic synapses
Tretter, V. E., Mukherjee, J. T., Maric, H. M., Schindelin, H., Sieghart, W., & Moss, S. J. (2012) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2012.00023 · Cited by 128 · see citing works
Distribution of α1, α4, γ2, and δ subunits of GABAA receptors in hippocampal granule cells
Sun, C., Sieghart, W., & Kapur, J. (2004) · Brain Research · doi:10.1016/j.brainres.2004.09.056 · Cited by 126 · see citing works
Gephyrin clustering is required for the stability of GABAergic synapses
Yu, W., Jiang, M., Miralles, C. P., Li, R., Chen, G., & Blas, A. L. D. (2007) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2007.08.008 · Cited by 125 · see citing works
Functional regulation of GABAA receptors in nervous system pathologies
Hines, R. M., Davies, P. A., Moss, S. J., & Maguire, J. L. (2011) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2011.10.007 · Cited by 125 · see citing works
Neurobiology and Therapeutic Potential of α5-GABA Type A Receptors
Jacob, T. C. (2019) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2019.00179 · Cited by 124 · see citing works
Identification and Characterization of hPEM-2, a Guanine Nucleotide Exchange Factor Specific for Cdc42
Reid, T., Bathoorn, A., Ahmadian, M. R., & Collard, J. G. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.47.33587 · Cited by 124 · see citing works
Dendrite-targeting interneurons control synaptic NMDA-receptor activation via nonlinear α5-GABAA receptors
Schulz, J. M., Knoflach, F., Hernandez, M., & Bischofberger, J. (2018) · Nature Communications · doi:10.1038/s41467-018-06004-8 · Cited by 115 · see citing works
Monoclonal antibodies and conventional antisera to the GABAA receptor/benzodiazepine receptor/Cl- channel complex
Vitórica, J., Park, D., Chin, G. J., & Blas, A. L. D. (1988) · Journal of Neuroscience · doi:10.1523/jneurosci.08-02-00615.1988 · Cited by 114 · see citing works
A Significant Part of Native γ-Aminobutyric AcidAReceptors Containing α4 Subunits Do Not Contain γ or δ Subunits
Bencsits, E., Ebert, V., Tretter, V. E., & Sieghart, W. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.28.19613 · Cited by 113 · see citing works
GABAergic Innervation Organizes Synaptic and Extrasynaptic GABAAReceptor Clustering in Cultured Hippocampal Neurons
Christie, S. B., Miralles, C. P., & Blas, A. L. D. (2002) · Journal of Neuroscience · doi:10.1523/jneurosci.22-03-00684.2002 · Cited by 108 · see citing works
Assembly of GABAA receptor subunits: analysis of transient single-cell expression utilizing a fluorescent substrate/marker gene technique
Angelotti, T. P., Uhler, M., & Macdonald, R. L. (1993) · Journal of Neuroscience · doi:10.1523/jneurosci.13-04-01418.1993 · Cited by 108 · see citing works
Gephyrin-mediated γ-Aminobutyric Acid Type A and Glycine Receptor Clustering Relies on a Common Binding Site*
Maric, H., Mukherjee, J., Tretter, V., Moss, S., & Schindelin, H. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.303412 · Cited by 106 · see citing works
The brefeldin A‐inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the β subunits of the GABA A receptors
Charych, E. I., Yu, W., Miralles, C. P., Serwanski, D. R., Li, X., Rubio, M. E., & Blas, A. L. D. (2004) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2004.02481.x · Cited by 104 · see citing works
Molecular Basis of the γ-Aminobutyric Acid A Receptor α3 Subunit Interaction with the Clustering Protein Gephyrin*
Tretter, V., Kerschner, B., Milenkovic, I., Ramsden, S., Ramerstorfer, J., Saiepour, L., Maric, H., Moss, S., Schindelin, H., Harvey, R., et al. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.291336 · Cited by 102 · see citing works
Disruption of postsynaptic GABAA receptor clusters leads to decreased GABAergic innervation of pyramidal neurons
Li, R., Yu, W., Christie, S. B., Miralles, C. P., Bai, J., LoTurco, J. J., & Blas, A. L. D. (2005) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2005.03426.x · Cited by 86 · see citing works
The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane
Weir, N. R., Kamber, R. A., Martenson, J. S., & Denic, V. (2017) · eLife · doi:10.7554/elife.28507 · Cited by 81 · see citing works
Input-Specific Synaptic Location and Function of the α5 GABA A Receptor Subunit in the Mouse CA1 Hippocampal Neurons
Magnin, E., Francavilla, R., Amalyan, S., Gervais, É., David, L. S., Luo, X., & Topolnik, L. (2018) · Journal of Neuroscience · doi:10.1523/jneurosci.0567-18.2018 · Cited by 81 · see citing works
GABAA Receptor α and γ Subunits Shape Synaptic Currents via Different Mechanisms
Dixon, C. L., Sah, P., Lynch, J. W., & Keramidas, A. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.514695 · Cited by 80 · see citing works
An Essential Role for the Tetraspanin LHFPL4 in the Cell-Type-Specific Targeting and Clustering of Synaptic GABAA Receptors
Davenport, E. C., Pendolino, V., Kontou, G., McGee, T. P., Sheehan, D. F., López‐Doménech, G., Farrant, M., & Kittler, J. T. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.09.025 · Cited by 80 · see citing works
Bergmann Glia GABAAReceptors Concentrate on the Glial Processes That Wrap Inhibitory Synapses
Riquelme, R., Miralles, C. P., & Blas, A. L. D. (2002) · Journal of Neuroscience · doi:10.1523/jneurosci.22-24-10720.2002 · Cited by 78 · see citing works
Direct binding of GABAA receptor β2 and β3 subunits to gephyrin
Kowalczyk, S., Winkelmann, A., Smolinsky, B., Förstera, B., Neundorf, I., Schwarz, G., & Meier, J. C. (2012) · European Journal of Neuroscience · doi:10.1111/ejn.12078 · Cited by 77 · see citing works
Subcellular Localization and Endocytosis of Homomeric γ2 Subunit Splice Variants of γ-Aminobutyric Acid Type A Receptors
Connolly, C. N., Uren, J. M., Thomas, P. A., Gorrie, G., Gibson, A., Smart, T. G., & Moss, S. J. (1999) · Molecular and Cellular Neuroscience · doi:10.1006/mcne.1999.0746 · Cited by 77 · see citing works
Collybistin is required for both the formation and maintenance of GABAergic postsynapses in the hippocampus
Papadopoulos, T. G., Eulenburg, V., Reddy‐Alla, S., Mansuy, I. M., Li, Y., & Betz, H. (2008) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2008.06.006 · Cited by 76 · see citing works
A prominent epitope on GABAA receptors is recognized by two different monoclonal antibodies
Ewert, M., Blas, A. L. D., Möhler, H., & Seeburg, P. H. (1992) · Brain Research · doi:10.1016/0006-8993(92)90368-j · Cited by 76 · see citing works
Clptm1 Limits Forward Trafficking of GABAA Receptors to Scale Inhibitory Synaptic Strength
Ge, Y., Kang, Y., Cassidy, R. M., Moon, K., Lewis, R. M., Wong, R., Foster, L. J., & Craig, A. M. (2018) · Neuron · doi:10.1016/j.neuron.2017.12.038 · Cited by 75 · see citing works
Organizers of inhibitory synapses come of age
Krueger‐Burg, D., Papadopoulos, T. G., & Brose, N. (2017) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2017.04.003 · Cited by 74 · see citing works
The Crystal Structure of Cdc42 in Complex with Collybistin II, a Gephyrin-interacting Guanine Nucleotide Exchange Factor
Xiang, S., Kim, E. Y., Connelly, J. J., Nassar, N. N., Kirsch, J., Winking, J., Schwarz, G., & Schindelin, H. (2006) · Journal of Molecular Biology · doi:10.1016/j.jmb.2006.03.019 · Cited by 73 · see citing works
Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission
Ghosh, H., Auguadri, L., Battaglia, S., Thirouin, Z. S., Zemoura, K., Messner, S., Acuña, M. A., Wildner, H., Yévenes, G. E., Dieter, A., et al. (2016) · Nature Communications · doi:10.1038/ncomms13365 · Cited by 72 · see citing works
Gephyrin: a key regulatory protein of inhibitory synapses and beyond
Groeneweg, F. L., Trattnig, C., Kuhse, J., Nawrotzki, R., & Kirsch, J. (2018) · Histochemistry and Cell Biology · doi:10.1007/s00418-018-1725-2 · Cited by 71 · see citing works
Immunocytochemical localization of the ?3 subunit of the ?-aminobutyric acidA receptor in the rat brain
Miralles, C. P., Li, M., Mehta, A. K., Khan, Z. U., & Blas, A. L. D. (1999) · The Journal of Comparative Neurology · doi:10.1002/(sici)1096-9861(19991101)413:4 · Cited by 71 · see citing works
SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localizes preferentially at post‐synaptic specializations of inhibitory synapses
Fukaya, M., Kamata, A., Hara, Y., Tamaki, H., Katsumata, O., Ito, N., Takeda, S., Hata, Y., Suzuki, T., Watanabe, M., et al. (2011) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2010.07167.x · Cited by 70 · see citing works
Collybistin splice variants differentially interact with gephyrin and Cdc42 to regulate gephyrin clustering at GABAergic synapses
Tyagarajan, S. K., Ghosh, H., Harvey, K., & Fritschy, J. (2011) · Journal of Cell Science · doi:10.1242/jcs.086199 · Cited by 70 · see citing works
Assembly rules for GABAA receptor complexes in the brain
Martenson, J. S., Yamasaki, T., Chaudhury, N. H., Albrecht, D. E., & Tomita, S. (2017) · eLife · doi:10.7554/elife.27443 · Cited by 69 · see citing works
Tuning GABAergic Inhibition: Gephyrin Molecular Organization and Functions
Pizzarelli, R., Griguoli, M., Zacchi, P., Petrini, E. M., Barberis, A., Cattaneo, A., & Cherubini, E. (2020) · Neuroscience · doi:10.1016/j.neuroscience.2019.07.036 · Cited by 69 · see citing works
Developmental seizures and mortality result from reducing GABAA receptor α2-subunit interaction with collybistin
Hines, R. M., Maric, H., Hines, D. J., Modgil, A., Panzanelli, P., Nakamura, Y., Nathanson, A. J., Cross, A., Deeb, T. Z., Brandon, N., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-05481-1 · Cited by 68 · see citing works
Septin 11 Is Present in GABAergic Synapses and Plays a Functional Role in the Cytoarchitecture of Neurons and GABAergic Synaptic Connectivity
Li, X., Serwanski, D. R., Miralles, C. P., Nagata, K., & Blas, A. L. D. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.008870 · Cited by 65 · see citing works
γ-Aminobutyric Acid Type A (GABAA) Receptor α Subunits Play a Direct Role in Synaptic Versus Extrasynaptic Targeting
Wu, X., Wu, Z., Ning, G., Guo, Y., Ali, R., Macdonald, R. L., Blas, A. L. D., Lüscher, B., & Chen, G. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.360461 · Cited by 62 · see citing works
Diffusion dynamics of synaptic molecules during inhibitory postsynaptic plasticity
Petrini, E. M., & Barberis, A. (2014) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2014.00300 · Cited by 62 · see citing works
Synaptic localization of α5 GABA (A) receptors via gephyrin interaction regulates dendritic outgrowth and spine maturation
Brady, M. L., & Jacob, T. C. (2015) · Developmental Neurobiology · doi:10.1002/dneu.22280 · Cited by 60 · see citing works
The GABAA Receptor β Subunit Is Required for Inhibitory Transmission
Nguyen, Q.-A., & Nicoll, R. A. (2018) · Neuron · doi:10.1016/j.neuron.2018.03.046 · Cited by 60 · see citing works
Structure–Function Relationships of Glycine and GABAA Receptors and Their Interplay With the Scaffolding Protein Gephyrin
Kasaragod, V. B., & Schindelin, H. (2018) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2018.00317 · Cited by 60 · see citing works
The Biochemical Anatomy of Cortical Inhibitory Synapses
Heller, E. A., Zhang, W., Selimi, F., Earnheart, J. C., Ślimak, M. A., Santos‐Torres, J., Ibáñez-Tallon, I., Aoki, C. J., Chait, B. T., & Heintz, N. (2012) · PLoS ONE · doi:10.1371/journal.pone.0039572 · Cited by 57 · see citing works
A Four PDZ Domain-containing Splice Variant Form of GRIP1 Is Localized in GABAergic and Glutamatergic Synapses in the Brain
Charych, E. I., Yu, W., Li, R., Serwanski, D. R., Miralles, C. P., Li, X., Yang, B. Y., Pinal, N., Walikonis, R. S., & Blas, A. L. D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m405786200 · Cited by 56 · see citing works
??5 Subunit-containing GABAA receptors form clusters at GABAergic synapses in hippocampal cultures
Christie, S. B., & Blas, A. L. D. (2002) · Neuroreport · doi:10.1097/00001756-200212030-00037 · Cited by 55 · see citing works
Two pools of Triton X‐100‐insoluble GABAA receptors are present in the brain, one associated to lipid rafts and another one to the post‐synaptic GABAergic complex
Li, X., Serwanski, D. R., Miralles, C. P., Bahr, B. A., & Blas, A. L. D. (2007) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2007.04635.x · Cited by 54 · see citing works
Identification of a Gephyrin-Binding Motif in the GDP/GTP Exchange Factor Collybistin
Grosskreutz, Y., Hermann, A., Kins, S., Fuhrmann, J. C., Betz, H., & Kneussel, M. (2001) · Biological chemistry · doi:10.1515/bc.2001.179 · Cited by 53 · see citing works
Postsynaptic plasticity of GABAergic synapses
Barberis, A. (2019) · Neuropharmacology · doi:10.1016/j.neuropharm.2019.05.020 · Cited by 53 · see citing works
Clustered and non-clustered GABAA receptors in cultured hippocampal neurons
Christie, S. B., Li, R.-W., Miralles, C. P., By, Y., & Blas, A. L. D. (2005) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2005.08.014 · Cited by 51 · see citing works
Molecular and Functional Interaction between Protocadherin-γC5 and GABAAReceptors
Li, Y., Xiao, H., Chiou, T., Jin, H., Bonhomme, B., Miralles, C. P., Pinal, N., Ali, R., Chen, W. V., Maniatis, T., et al. (2012) · Journal of Neuroscience · doi:10.1523/jneurosci.0969-12.2012 · Cited by 50 · see citing works
Self-citation
Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged γ-Aminobutyric Acid Receptor, Type A (GABAA), α2 Subunit Knock-in Mouse*
Nakamura, Y., Morrow, D. H., Modgil, A., Huyghe, D., Deeb, T. Z., Lumb, M., Davies, P., & Moss, S. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m116.724443 · Cited by 50 · see citing works
Differential Regulation of the Postsynaptic Clustering of γ-Aminobutyric Acid Type A (GABAA) Receptors by Collybistin Isoforms
Chiou, T., Bonhomme, B., Jin, H., Miralles, C. P., Xiao, H., Fu, Z., Harvey, R. J., Harvey, K., Vicini, S., & Blas, A. L. D. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.236190 · Cited by 49 · see citing works
Self-citation
Synaptic and nonsynaptic localization of protocadherin‐γC5 in the rat brain
Li, Y., Serwanski, D. R., Miralles, C. P., Fiondella, C. G., LoTurco, J. J., Rubio, M. E., & Blas, A. L. D. (2010) · The Journal of Comparative Neurology · doi:10.1002/cne.22390 · Cited by 44 · see citing works
Chapter 13 Synaptic and extrasynaptic GABAA receptor and gephyrin clusters
Christie, S. B., Li, R.-W., Miralles, C. P., Riquelme, R., Yang, B. Y., Charych, E. I., Yu, W., Daniels, S. B., Cantino, M. E., & Blas, A. L. D. (2002) · Progress in brain research · doi:10.1016/s0079-6123(02)36015-1 · Cited by 43 · see citing works
Gephyrin and the regulation of synaptic strength and dynamics at glycinergic inhibitory synapses
Álvarez, F. J. (2016) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2016.09.003 · Cited by 40 · see citing works
Impairment of Inhibitory Synapse Formation and Motor Behavior in Mice Lacking the NL2 Binding Partner LHFPL4/GARLH4
Wu, M., Tian, H.-L., Liu, X., Lai, J. H. C., Du, S., & Xia, J. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.04.015 · Cited by 40 · see citing works
The α 4 Subunit of the GABA A Receptors from Rat Brain and Retina
Khan, Z. U., Gutiérrez, A., MEHTA, A. K., Miralles, C. P., & Blas, A. L. D. (1996) · Neuropharmacology · doi:10.1016/s0028-3908(96)00033-0 · Cited by 39 · see citing works
Endosomal Phosphatidylinositol 3-Phosphate Promotes Gephyrin Clustering and GABAergic Neurotransmission at Inhibitory Postsynapses*♦
Papadopoulos, T. G., Rhee, H., Subramanian, D., Paraskevopoulou, F., Mueller, R., Schultz, C., Brose, N., Rhee, J., & Betz, H. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m116.771592 · Cited by 36 · see citing works
Gastric Bezoars after Partial Gastrectomy Report of Five Cases
Szemes, G. C., & Amberg, J. R. (1968) · Radiology · doi:10.1148/90.4.765 · Cited by 35 · see citing works
Ring Finger Protein 34 (RNF34) Interacts with and Promotes γ-Aminobutyric Acid Type-A Receptor Degradation via Ubiquitination of the γ2 Subunit
Jin, H., Chiou, T., Serwanski, D. R., Miralles, C. P., Pinal, N., & Blas, A. L. D. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.603068 · Cited by 33 · see citing works
Self-citation
The Short Splice Variant of the γ2 Subunit Acts as an External Modulator of GABA A Receptor Function
Boileau, A. J., Pearce, R. A., & Czajkowski, C. (2010) · Journal of Neuroscience · doi:10.1523/jneurosci.5039-09.2010 · Cited by 33 · see citing works
IQ Motif and SEC7 Domain-containing Protein 3 (IQSEC3) Interacts with Gephyrin to Promote Inhibitory Synapse Formation*
Um, J., Choii, G., Park, D., Kim, D., Jeon, S., Kang, H., Mori, T., Papadopoulos, T. G., Yoo, T., Lee, Y., et al. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.712893 · Cited by 32 · see citing works
Gephyrin expression and clustering affects the size of glutamatergic synaptic contacts
Yu, W., & Blas, A. L. D. (2007) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2007.05014.x · Cited by 30 · see citing works
Comparison of γ-Aminobutyric Acid, Type A (GABAA), Receptor αβγ and αβδ Expression Using Flow Cytometry and Electrophysiology
Botzolakis, E. J., Gurba, K. N., Lagrange, A. H., Feng, H.-J., Stanic, A. K., Hu, N., & Macdonald, R. L. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.698860 · Cited by 29 · see citing works
GABAA receptor subunit composition and competition at synapses are tuned by GABAB receptor activity
Gerrow, K., & Triller, A. (2014) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2014.04.001 · Cited by 28 · see citing works
Distinct synaptic localization patterns of brefeldin A‐resistant guanine nucleotide exchange factors BRAG2 and BRAG3 in the mouse retina
Sakagami, H., Katsumata, O., Hara, Y., Tamaki, H., Watanabe, M., Harvey, R. J., & Fukaya, M. (2013) · The Journal of comparative neurology · doi:10.1002/cne.23206 · Cited by 28 · see citing works
Chemogenetic Isolation Reveals Synaptic Contribution of δ GABAAReceptors in Mouse Dentate Granule Neurons
Sun, M., Shu, H., Benz, A. M., Bracamontes, J. R., Akk, G., Zorumski, C. F., Steinbach, J. H., & Mennerick, S. J. (2018) · Journal of Neuroscience · doi:10.1523/jneurosci.0799-18.2018 · Cited by 27 · see citing works
Sequences Flanking the Gephyrin-Binding Site of GlyRβ Tune Receptor Stabilization at Synapses
Grünewald, N., Jan, A., Salvatico, C., Kress, V., Renner, M. L., Triller, A., Specht, C. G., & Schwarz, G. (2018) · eNeuro · doi:10.1523/eneuro.0042-17.2018 · Cited by 27 · see citing works
Fractional occupancy of synaptic binding sites and the molecular plasticity of inhibitory synapses
Specht, C. G. (2019) · Neuropharmacology · doi:10.1016/j.neuropharm.2019.01.008 · Cited by 25 · see citing works
Structure of Heteropentameric GABAA Receptors and Receptor-Anchoring Properties of Gephyrin
Kasaragod, V. B., & Schindelin, H. (2019) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2019.00191 · Cited by 24 · see citing works
Behavioural correlates of an altered balance between synaptic and extrasynaptic GABAAergic inhibition in a mouse model
Sinkkonen, S. T., Vekovischeva, O. Y., Möykkynen, T. P., Ogris, W., Sieghart, W., Wisden, W., & Korpi, E. R. (2004) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2004.03684.x · Cited by 23 · see citing works
Modulation of Gephyrin-Glycine Receptor Affinity by Multivalency
Maric, H. M., Kasaragod, V. B., & Schindelin, H. (2014) · ACS Chemical Biology · doi:10.1021/cb500303a · Cited by 23 · see citing works
Design and Synthesis of High‐Affinity Dimeric Inhibitors Targeting the Interactions between Gephyrin and Inhibitory Neurotransmitter Receptors
Maric, H. M., Kasaragod, V. B., Haugaard‐Kedström, L. M., Hausrat, T. J., Kneussel, M., Schindelin, H., & Strømgaard, K. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201409043 · Cited by 23 · see citing works
Selective localization of collybistin at a subset of inhibitory synapses in brain circuits
Patrizi, A., Viltono, L., Frola, E., Harvey, K., Harvey, R., & Sassoè-Pognetto, M. (2012) · The Journal of comparative neurology · doi:10.1002/cne.22702 · Cited by 22 · see citing works
In vivo clonal overexpression of neuroligin 3 and neuroligin 2 in neurons of the rat cerebral cortex: Differential effects on GABAergic synapses and neuronal migration
Fekete, C. D., Chiou, T., Miralles, C. P., Harris, R., Fiondella, C. G., LoTurco, J. J., & Blas, A. L. D. (2015) · The Journal of Comparative Neurology · doi:10.1002/cne.23740 · Cited by 21 · see citing works
Self-citation
Gephyrin interacts with the glutamate receptor interacting protein 1 isoforms at GABAergic synapses
Yu, W., Charych, E. I., Serwanski, D. R., Li, R., Ali, R., Bahr, B. A., & Blas, A. L. D. (2008) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2008.05311.x · Cited by 21 · see citing works
Specificity of Collybistin-Phosphoinositide Interactions
Ludolphs, M., Schneeberger, D., Soykan, T., Schäfer, J., Papadopoulos, T. G., Brose, N., Schindelin, H., & Steinem, C. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.673400 · Cited by 20 · see citing works
Cell surface expression of homomeric GABAA receptors depends on single residues in subunit transmembrane domains
Hannan, S. B., & Smart, T. G. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra118.002792 · Cited by 19 · see citing works
GABA beyond the synapse: defining the subtype‐specific pharmacodynamics of non‐synaptic GABAAreceptors
Lagrange, A. H., Hu, N., & Macdonald, R. L. (2018) · The Journal of Physiology · doi:10.1113/jp276187 · Cited by 19 · see citing works
Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures
Nathanson, A. J., Zhang, Y., Smalley, J. L., Ollerhead, T. A., Santos, M. A. R., Andrews, P. M., Wobst, H. J., Moore, Y. E., Brandon, N. J., Hines, R. M., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.06.014 · Cited by 19 · see citing works
The catalytic function of the gephyrin‐binding protein IQSEC3 regulates neurotransmitter‐specific matching of pre‐ and post‐synaptic structures in primary hippocampal cultures
Früh, S. P., Tyagarajan, S. K., Campbell, B. F. N., Bosshard, G., & Fritschy, J. (2018) · Journal of Neurochemistry · doi:10.1111/jnc.14572 · Cited by 16 · see citing works
In vivo transgenic expression of collybistin in neurons of the rat cerebral cortex
Fekete, C. D., Goz, R. U., Dinallo, S., Miralles, C. P., Chiou, T., Bear, J., Fiondella, C. G., LoTurco, J. J., & Blas, A. L. D. (2016) · The Journal of Comparative Neurology · doi:10.1002/cne.24137 · Cited by 14 · see citing works
Self-citation
Cellular Mechanisms Contributing to the Functional Heterogeneity of GABAergic Synapses
Campbell, B. F. N., & Tyagarajan, S. K. (2019) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2019.00187 · Cited by 12 · see citing works
Cytoplasmic domain of δ subunit is important for the extra-synaptic targeting of GABAAreceptor subtypes
Arslan, A., von Engelhardt, J., & Wisden, W. (2014) · Journal of Integrative Neuroscience · doi:10.1142/s0219635214500228 · Cited by 10 · see citing works
Legal issues of nursing practice.
Tt, C., & Jb, L. (1984) · PubMed · Cited by 1 · see citing works
[The effect of acupuncture on the abnormality of central cardiovascular function and analysis of pathways].
Yp, Z., & Zm, H. (1985) · PubMed · Cited by 1 · see citing works
Activated radixin is essential for GABAA receptor α5 subunit anchoring at the actin cytoskeleton
Loebrich, S., Bähring, R., Katsuno, T., & Kneussel, M. (2006) · Institutional Repositories DataBase (IRDB) · doi:10.1038/sj.emboj.7600995

Flagged first, then by citations

3 of the 147 references couldn’t be matched to a known paper.

Sorted by citations

GABA Regulates Electrical Activity and Tumor Initiation in Melanoma
Tagore, M. M., Hergenreder, E., Perlee, S. C., Cruz, N. M., Menocal, L., Suresh, S., Chan, E., Baron, M., Melendez, S., Dave, A., et al. (2023) · Cancer Discovery · doi:10.1158/2159-8290.cd-23-0389 · Cited by 46 · see citing works
Molecular Physiology of the Neuronal Synapse
Ramírez-Expósito, M. J., Cueto-Ureña, C., & Martínez-Martos, J. M. (2026) · Current Issues in Molecular Biology · doi:10.3390/cimb48010088 · Cited by 6 · see citing works
“Gephyrin is a multifunctional scaffold protein that forms submembranous postsynaptic planar lattices at both GABAergic and glycinergic synapses [57].”
Influential
Endophilin A1 facilitates organization of the GABAergic postsynaptic machinery to maintain excitation-inhibition balancePreprint
Chen, X., Pan, D., Liu, J., & Yang, Y. (2024) · eLife · doi:10.7554/elife.102792 · Cited by 2 · see citing works
Endophilin A1 facilitates organization of the GABAergic postsynaptic machinery to maintain excitation-inhibition balance
Chen, X., Pan, D., Liu, J., & Yang, Y. (2025) · eLife · doi:10.7554/elife.102792.4
Endophilin A1 facilitates organization of the GABAergic postsynaptic machinery to maintain excitation-inhibition balancePreprint
Chen, X., Pan, D., Liu, J., & Yang, Y. (2024) · eLife · doi:10.7554/elife.102792.1
Endophilin A1 facilitates organization of the GABAergic postsynaptic machinery to maintain excitation-inhibition balancePreprint
Chen, X., Pan, D., Liu, J., & Yang, Y. (2025) · eLife · doi:10.7554/elife.102792.2
Endophilin A1 facilitates organization of the GABAergic postsynaptic machinery to maintain excitation-inhibition balancePreprint
Chen, X., Pan, D., Liu, J., & Yang, Y. (2025) · eLife · doi:10.7554/elife.102792.3
Re-Evaluation of the Ultrastructural Localization of Tonic GABA-A Receptors
Rosas‐Arellano, A. (2025) · Pharmaceuticals · doi:10.3390/ph19010025

Reconstitution of GABAergic Postsynapses in Host Cells
Kanamalla, K. (2018) · OpenCommons - UConn (University of Connecticut)
Self-citation
GABAAR isoform and subunit structural motifs determine synaptic and extrasynaptic receptor localisation
Hannan, S. B., Minère, M., Harris, J., Izquierdo, P., Thomas, P., Tench, B., & Smart, T. G. (2019) · Neuropharmacology · doi:10.1016/j.neuropharm.2019.02.022 · Cited by 52 · see citing works
Loss of Postsynaptic GABAA Receptor Clustering in Gephyrin-Deficient Mice
Kneussel, M., Brandstätter, J., Laube, B., Stahl, S., Müller, U., & Betz, H. (1999) · Journal of Neuroscience · doi:10.1523/jneurosci.19-21-09289.1999 · Cited by 456 · see citing works
Gephyrin, a major player in GABAergic postsynaptic membrane assembly?
Betz, H. (1998) · Nature Neuroscience · doi:10.1038/2777 · Cited by 51 · see citing works
γ-Aminobutyric Acid Type A (GABAA) Receptor α Subunits Play a Direct Role in Synaptic Versus Extrasynaptic Targeting
Wu, X., Wu, Z., Ning, G., Guo, Y., Ali, R., Macdonald, R. L., Blas, A. L. D., Lüscher, B., & Chen, G. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.360461 · Cited by 62 · see citing works

Authors: George, S., Chiou, T.-T., Kanamalla, K., & De Blas, A. L.
Affiliations: University of Connecticut (US)
ORCID: George, Shanu
Chiou, Tzu-Ting
De Blas, Angel L.
Venue: Cellular and Molecular Neurobiology
Publisher: Springer Science and Business Media LLC
Published: 2021-02-05
Volume: 42
Issue: 5
Pages: 1585-1604
ISSN: 0272-4340 (ISSN-L)
1573-6830
DOI: 10.1007/s10571-021-01050-1
PMC: PMC11421751
OpenAlex: W3126404023
Cited by: 8
References: 147
Citations by year: 2026: 1 · 2025: 4 · 2024: 2 · 2023: 1
FWCI: 0.35
Type: journal article
Language: en
Update notices: correction (2021-02-24)
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Cellular and Molecular Neurobiology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institute of Neurological Disorders and Stroke (R01NS038752)
University of Connecticut (Research Incentive Program Grant)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
Keywords: GABAA receptor, gephyrin, collybistin, extrasynaptic GABAA receptors, synaptic localization, receptor subunit composition, tonic inhibition, phasic inhibition, receptor reconstitution, HEK293 cells
MeSH terms: Cell Membrane, gamma-Aminobutyric Acid, Humans, Membrane Proteins, Receptors, GABA-A, Synapses, Receptors, N-Methyl-D-Aspartate, HEK293 Cells, Rho Guanine Nucleotide Exchange Factors

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Recruitment of Plasma Membrane GABA-A Receptors by Submembranous Gephyrin/Collybistin Clusters

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Cites

Cited by

Related

Record

About these checks