Cell-free DNA and telomere length among women undergoing in vitro fertilization treatment
Open accessAbstract
Purpose The current research is aimed at finding potential non-invasive bio-markers that will help us learn more about the mechanisms at play in failed assisted reproduction treatment. This exploratory pilot study examined the relationship between cell-free DNA (CFD) in plasma and telomere length in lymphocytes among women undergoing in vitro fertilization (IVF) and compared telomere length and CFD levels to a healthy control group.Methods Blood of 20 women undergoing IVF was collected at three time points during the IVF cycle. We assessed the relationship between CFD and telomere length as well as controlling for morning cortisol levels. We also collected blood of 10 healthy controls at two time points (luteal and follicular phases of the menstrual cycle) and compared mean telomere length, CFD, and cortisol levels between the IVF patients and healthy controls.Results The results revealed an inverse relationship between CFD levels and telomere lengths at several time points that remained significant even after controlling for cortisol levels. Women undergoing IVF had statistically significant higher levels of CFD and shorter telomeres compared to healthy controls.Conclusions The relationship between telomere length and CFD should be further explored in larger studies in order to uncover potential mechanisms that cause both shortened telomere length and elevated CFD in women undergoing IVF.
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Telomere shortening is associated with reduced fertility in several ways.
“Telomere shortening has been associated with reduced fertility in several ways177–180.”
From Telomere dysfunction in ageing and age-related diseases (2022) · cites this paper as [177]
Women undergoing in vitro fertilization tend to have shorter leukocyte telomeres than healthy individuals.
“Women undergoing in vitro fertilization tend to have shorter leukocyte telomere length than healthy individuals177”
From Telomere dysfunction in ageing and age-related diseases (2022) · cites this paper as [177]
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Cites
The serial cultivation of human diploid cell strains
Hayflick, L., & Moorhead, P. S. (1961) · Experimental Cell Research · doi:10.1016/0014-4827(61)90192-6 · Cited by 8,508 · see citing works
Telomeres shorten during ageing of human fibroblasts
Harley, C. B., Futcher, A. B., & Greider, C. W. (1990) · Nature · doi:10.1038/345458a0 · Cited by 5,750 · see citing works
McEWEN, B. S., & SEEMAN, T. (1999) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1999.tb08103.x · Cited by 1,824 · see citing works
Regulation of the Hypothalamic-Pituitary-Adrenal Axis by Cytokines: Actions and Mechanisms of Action
TURNBULL, A. V., & RIVIER, C. L. (1999) · Physiological Reviews · doi:10.1152/physrev.1999.79.1.1 · Cited by 1,297 · see citing works
Women’s emotional adjustment to IVF: a systematic review of 25 years of research
Verhaak, C. M., Smeenk, J. M. J., Evers, A. W. M., Kremer, J. A. M., Kraaimaat, F., & Braat, D. D. M. (2006) · Human Reproduction Update · doi:10.1093/humupd/dml040 · Cited by 672 · see citing works
Kudielka, B. M., & Wüst, S. (2010) · Stress · doi:10.3109/10253890902874913 · Cited by 424 · see citing works
Telomeres and early-life stress: an overview
Price, L. H., Kao, H.-T., Burgers, D. E., Carpenter, L. L., & Tyrka, A. R. (2013) · Biological Psychiatry · doi:10.1016/j.biopsych.2012.06.025 · Cited by 339 · see citing works
O’Donovan, A., Pantell, M. S., Puterman, E. M., Dhabhar, F. S., Blackburn, E. H., Yaffe, K. S., Cawthon, R., Opresko, P. L., Hsueh, W.-C., Satterfield, S., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0019687 · Cited by 338 · see citing works
The autoimmune bases of infertility and pregnancy loss
Carp, H., Selmi, C. F., & Shoenfeld, Y. (2012) · Journal of Autoimmunity · doi:10.1016/j.jaut.2011.11.016 · Cited by 228 · see citing works
Does cellular aging relate to patterns of allostasis?
Tomiyama, A. J., O’Donovan, A., Lin, J., Puterman, E. M., Lazaro, A., Chan, J. L., Dhabhar, F. S., Wolkowitz, O. M., Kirschbaum, C., Blackburn, E. H., et al. (2011) · Physiology & Behavior · doi:10.1016/j.physbeh.2011.11.016 · Cited by 206 · see citing works
Wikgren, M., Maripuu, M., Karlsson, T., Nordfjäll, K., Bergdahl, J., Hultdin, J., Del‐Favero, J., Roos, G., Nilsson, L., Adolfsson, R., et al. (2011) · Biological Psychiatry · doi:10.1016/j.biopsych.2011.09.015 · Cited by 173 · see citing works
Zhong, X. Y., Holzgreve, W., & Hahn, S. (2002) · Hypertension in Pregnancy · doi:10.1081/prg-120002911 · Cited by 171 · see citing works
O’Donovan, A., Tomiyama, A. J., Lin, J., Puterman, E. M., Adler, N. E., Kemeny, M. E., Wolkowitz, O. M., Blackburn, E. H., & Epel, E. S. (2012) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2012.01.007 · Cited by 149 · see citing works
Stress and outcome success in IVF: the role of self-reports and endocrine variables
Smeenk, J. M. J., Verhaak, C. M., Vingerhoets, A. J. J. M., Sweep, F. C. G. J., Merkus, J. M. W. M., Willemsen, S., van Minnen, A., Straatman, H., & Braat, D. D. M. (2005) · Human Reproduction · doi:10.1093/humrep/deh739 · Cited by 149 · see citing works
Cell-Free DNA as a Noninvasive Acute Rejection Marker in Renal Transplantation
Moreira, V. G., García, B. P., Martín, J. M. B., et al. (2009) · Clinical Chemistry · doi:10.1373/clinchem.2009.129072 · Cited by 148 · see citing works
Telomeres and human reproduction
Kalmbach, K., Antunes, D. M. F., Dracxler, R. C., Knier, T. W., Seth-Smith, M. L., Wang, F., Liu, L., & Keefe, D. L. (2012) · Fertility and Sterility · doi:10.1016/j.fertnstert.2012.11.039 · Cited by 137 · see citing works
Massive Telomere Loss Is an Early Event of DNA Damage-induced Apoptosis
Ramı́rez, R., Carracedo, J., Jiménez, R., Canela, A., Herrera, E., Aljama, P., & Blasco, M. A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m206818200 · Cited by 94 · see citing works
Apoptotic cell-free DNA promotes inflammation in haemodialysis patients
Atamaniuk, J., Kopecky, C., Skoupy, S., et al. (2011) · Nephrology Dialysis Transplantation · doi:10.1093/ndt/gfr695 · Cited by 94 · see citing works
Zhang, R., Shao, F., Wu, X., & Ying, K. (2009) · Lung Cancer · doi:10.1016/j.lungcan.2009.11.009 · Cited by 83 · see citing works
Cell-Free Fetal DNA, Telomeres, and the Spontaneous Onset of Parturition
Phillippe, M. (2015) · Reproductive Sciences · doi:10.1177/1933719115592714 · Cited by 76 · see citing works
Cell free DNA detected by a novel method in acute ST-elevation myocardial infarction patients
Shimony, A., Zahger, D., Gilutz, H., et al. (2010) · Acute Cardiac Care · doi:10.3109/17482941.2010.513732 · Cited by 72 · see citing works
Traver, S., Assou, S., Scalici, E., Haouzi, D., Al-Edani, T., Belloc, S., & Hamamah, S. (2014) · Human Reproduction Update · doi:10.1093/humupd/dmu031 · Cited by 66 · see citing works
Telomeres, telomerase, and apoptosis
Mondello, C., & Scovassi, A. I. (2004) · Biochemistry and Cell Biology · doi:10.1139/o04-048 · Cited by 66 · see citing works
Cell-free DNA in human follicular fluid as a biomarker of embryo quality
Scalici, E., Traver, S., Molinari, N., et al. (2014) · Human Reproduction · doi:10.1093/humrep/deu238 · Cited by 54 · see citing works
Cell-free DNA levels in acute myocardial infarction patients during hospitalization
Destouni, A., Vrettou, C., Antonatos, D., Chouliaras, G., Traeger-Synodinos, J., Patsilinakos, S., Kitsiou-Tzeli, S., Tsigas, D., & Kanavakis, E. (2009) · Acta Cardiologica · doi:10.2143/ac.64.1.2034362 · Cited by 51 · see citing works
Czamanski‐Cohen, J., Sarid, O., Cwikel, J., Levitas, E., Lunenfeld, E., Douvdevani, A., & Har‐Vardi, I. (2014) · Archives of Women s Mental Health · doi:10.1007/s00737-013-0407-2 · Cited by 34 · see citing works
Self-citationXANTHINE OXIDASE ACTIVATION IN MILD GESTATIONAL HYPERTENSION
Németh, I., Tálosi, G., Papp, A., & BODA, D. (2002) · Hypertension in Pregnancy · doi:10.1081/prg-120002905 · Cited by 33 · see citing works
Czamanski‐Cohen, J., Sarid, O., Cwikel, J., et al. (2012) · Reproductive BioMedicine Online · doi:10.1016/j.rbmo.2012.09.018 · Cited by 29 · see citing works
Self-citationOka (1994) · Oncology Reports · doi:10.3892/or_00000071 · Cited by 25 · see citing works
Both Maternal and Fetal Cell‐Free DNA in Plasma Fluctuate
Hahn, S., Zhong, X. Y., Bürk, M. R., et al. (2001) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2001.tb03875.x · Cited by 19 · see citing works
O'Donovan, F., Capobianco, L., Taylor-Bennett, J., & Adrian Wells, A. (2025) · PLoS ONE · doi:10.1371/journal.pone.0309683 · Cited by 13 · see citing works
Hart, E. A., Patton, W. C., Jacobson, J. D., et al. (2005) · Journal of Assisted Reproduction and Genetics · doi:10.1007/s10815-005-4924-4 · Cited by 11 · see citing works
Cell-Free DNA in Maternal Plasma: Has the Size-Distribution Puzzle Been Solved?
Hahn, S., & Zimmermann, B. G. (2010) · Clinical Chemistry · doi:10.1373/clinchem.2010.149609 · Cited by 7 · see citing works
Taking Stock of Telomere Length at Telome Health
McCarthy, A. A. (2011) · Chemistry & Biology · doi:10.1016/j.chembiol.2011.08.004 · Cited by 3 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- [17]17. Hayflick L, Moorhead P. The human fibroblast and a new model for cellular senescence. Exp Cell Res. 1961;25:585-8.
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Telomere dysfunction in ageing and age-related diseases
Rossiello, F., Jurk, D., Passos, J. F., & d’Adda di Fagagna, F. (2022) · Nature Cell Biology · doi:10.1038/s41556-022-00842-x · Cited by 754 · see citing works
Reproductive aging and telomeres: Are women and men equally affected?
Chico-Sordo, L., Córdova-Oriz, I., Polonio, A. M., S-Mellado, L. S., Medrano, M., García-Velasco, J. A., & Varela, E. (2021) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2021.111541 · Cited by 27 · see citing works
- Authors
- Czamanski-Cohen, J., Sarid, O., Cwikel, J., Douvdevani, A., Levitas, E., Lunenfeld, E., & Har-Vardi, I.
- Affiliations
- Ben-Gurion University of the Negev (IL)
- Venue
- Journal of Assisted Reproduction and Genetics
- Published
- 2015-10-05
- Volume
- 32
- Issue
- 11
- Pages
- 1697-1703
- ISSN
- 0740-7769 (ISSN-L)
- 1058-0468
- 1573-7330
- PMC
- PMC4651952
- OpenAlex
- W2177539334
- Cited by
- 19
- References
- 34
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 3
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Assisted Reproduction and Genetics (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (CA078447-14, R25CA078447)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Health and Technologies
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Keywords
- cell-free DNA, telomere length, in vitro fertilization, non-invasive biomarkers, plasma cell-free DNA, cortisol, menstrual cycle phases
- MeSH terms
- Adolescent, Adult, DNA, Female, Fertilization in Vitro, Humans, Hydrocortisone, Infertility, Female, Lymphocytes, Case-Control Studies, Telomere, Young Adult, Telomere Homeostasis
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