Cited by 13Mitochondrial Function and PathologyCoenzyme Q10 studies and effectsRedox biology and oxidative stress

Long chain fatty acyl-CoA modulation of H2O2 release at mitochondrial complex I

Bortolami, Silvia, Comelato, Evelin, Zoccarato, Franco, Alexandre, Adolfo, Cavallini, Lucia·2008·Journal of Bioenergetics and Biomembranes

Abstract

Complex I is responsible for most of the mitochondrial H(2)O(2) release, low during the oxidation of the NAD linked substrates and high during succinate oxidation, via reverse electron flow. This H(2)O(2) production appear physiological since it occurs at submillimolar concentrations of succinate also in the presence of NAD substrates in heart (present work) and rat brain mitochondria (Zoccarato et al., Biochem J, 406:125-129, 2007). Long chain fatty acyl-CoAs, but not fatty acids, act as strong inhibitors of succinate dependent H(2)O(2) release. The inhibitory effect of acyl-CoAs is independent of their oxidation, being relieved by carnitine and unaffected or potentiated by malonyl-CoA. The inhibition appears to depend on the unbound form since the acyl-CoA effect decreases at BSA concentrations higher than 2 mg/ml; it is not dependent on DeltapH or Deltap and could depend on the inhibition of reverse electron transfer at complex I, since palmitoyl-CoA inhibits the succinate dependent NAD(P) or acetoacetate reduction.

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Crossref · 10.1007/s10863-008-9126-1

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Authors
Bortolami, S., Comelato, E., Zoccarato, F., Alexandre, A., & Cavallini, L.
Affiliations
University of Padua (IT)
Corresponding author
Cavallini, Lucia
Venue
Journal of Bioenergetics and Biomembranes
Published
2008-01-24
Volume
40
Issue
1
Pages
9-18
ISSN
  • 0145-479X (ISSN-L)
  • 1573-6881
OpenAlex
W1992123175
Cited by
13
References
40
Citations by year
2016: 1 · 2014: 1 · 2013: 1
FWCI
1.37
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Bioenergetics and Biomembranes (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Coenzyme Q10 studies and effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
long-chain fatty acyl-CoA, mitochondrial complex I, hydrogen peroxide release, succinate oxidation, reverse electron transfer, NAD-linked substrates, palmitoyl-CoA, L-carnitine, malonyl-CoA, heart mitochondria
MeSH terms
Animals, Carnitine, Electron Transport, Hydrogen Peroxide, Malonyl Coenzyme A, Mitochondria, Heart, NADP, Oxidation-Reduction, Succinic Acid, Electron Transport Complex I, Rats

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