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Direct evidence of abca1-mediated efflux of cholesterol at the mouse blood–brain barrier

Do, Tuan Minh, Ouellet, Mélissa, Calon, Frédéric, Chimini, Giovanna, Chacun, Hélène, Farinotti, Robert, Bourasset, Fanchon·2011·Molecular and Cellular Biochemistry
DOI 10.1007/s11010-011-0910-6
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Abstract

We investigated the expression and function of Abca1 in wild-type C57BL/6, abca1(+/+), and abca1(-/-) mice brain capillaries forming the blood-brain barrier (BBB). We first demonstrated by quantitative RT-PCR and Western immunoblot that Abca1 was expressed and enriched in the wild-type mouse brain capillaries. In abca1(-/-) mice, we reported that the lack of Abca1 resulted in an 1.6-fold increase of the Abcg4 expression level compared to abca1(+/+) mice. Next, using the in situ brain perfusion technique, we showed that the [(3)H]cholesterol brain uptake clearance (Cl(up), μl/s/g brain), was significantly increased (107%) in abca1(-/-) mice compared to abca1(+/+) mice, meaning that the deficiency of Abca1 conducted to a significant decrease of the cholesterol efflux at the BBB level. In addition, the co-perfusion of probucol (Abca1 inhibitor) with [(3)H]cholesterol resulted in an increase of [(3)H]cholesterol Cl(up) (115%) in abca1(+/+) but not in abca1(-/-) mice, meaning that probucol inhibited selectively the efflux function of Abca1. In conclusion, our results demonstrated that Abca1 was expressed in the mouse brain capillaries and that Abca1 functions as an efflux transporter through the mouse BBB.

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Research works printed but not linked: 40

  1. Krimbou L, Denis M, Haidar B, Carrier M, Marcil M, Genest J Jr (2004) Molecular interactions between apoE and ABCA1: impact on apoE lipidation. J Lipid Res 45(5):839–848 · DOI · Paper page
  2. Klaassen CD, Aleksunes LM (2010) Xenobiotic, bile acid, and cholesterol transporters: function and regulation. Pharmacol Rev 62(1):1–96 · DOI
  3. Wellington CL, Walker EK, Suarez A, Kwok A, Bissada N, Singaraja R, Yang YZ, Zhang LH, James E, Wilson JE, Francone O, McManus BM, Hayden MR (2002) ABCA1 mRNA and protein distribution patterns predict multiple different roles and levels of regulation. Lab Invest 82(3):273–283 · DOI
  4. Karasinska JM, Rinninger F, Lutjohann D, Ruddle P, Franciosi S, Kruit JK, Singaraja RR, Hirsch-Reinshagen V, Fan J, Brunham LR, Bissada N, Ramakrishnan R, Wellington CL, Parks JS, Hayden MR (2009) Specific loss of brain ABCA1 increases brain cholesterol uptake and influences neuronal structure and function. J Neurosci 29(11):3579–3589 · DOI · Paper page
  5. Ohtsuki S, Watanabe Y, Hori S, Suzuki H, Bhongsatiern J, Fujiyoshi M, Kamoi M, Kamiya N, Takanaga H, Terasaki T (2004) mRNA expression of the ATP-binding cassette transporter subfamily A (ABCA) in rat and human brain capillary endothelial cells. Biol Pharm Bull 27(9):1437–1440 · DOI
  6. Dutheil F, Jacob A, Dauchy S, Beaune P, Scherrmann JM, Decleves X, Loriot MA (2010) ABC transporters and cytochromes P450 in the human central nervous system: influence on brain pharmacokinetics and contribution to neurodegenerative disorders. Expert Opin Drug Metab Toxicol 6(10):1161–1174 · DOI
  7. Nicolazzo JA, Katneni K (2009) Drug transport across the blood–brain barrier and the impact of breast cancer resistance protein (ABCG2). Curr Top Med Chem 9(2):130–147 · DOI
  8. Ueno M, Nakagawa T, Wu B, Onodera M, Huang CL, Kusaka T, Araki N, Sakamoto H (2010) Transporters in the brain endothelial barrier. Curr Med Chem 17(12):1125–1138 · DOI
  9. Urquhart BL, Kim RB (2009) Blood–brain barrier transporters and response to CNS-active drugs. Eur J Clin Pharmacol 65(11):1063–1070 · DOI
  10. Burns MP, Vardanian L, Pajoohesh-Ganji A, Wang L, Cooper M, Harris DC, Duff K, Rebeck GW (2006) The effects of ABCA1 on cholesterol efflux and Abeta levels in vitro and in vivo. J Neurochem 98(3):792–800 · DOI · Paper page
  11. Koldamova R, Fitz NF, Lefterov I (2010) The role of ATP-binding cassette transporter A1 in Alzheimer’s disease and neurodegeneration. Biochim Biophys Acta 1801(8):824–830 · DOI · Paper page
  12. Xiong H, Callaghan D, Jones A, Walker DG, Lue LF, Beach TG, Sue LI, Woulfe J, Xu H, Stanimirovic DB, Zhang W (2008) Cholesterol retention in Alzheimer’s brain is responsible for high beta- and gamma-secretase activities and Abeta production. Neurobiol Dis 29(3):422–437 · DOI
  13. Grimm MO, Grimm HS, Tomic I, Beyreuther K, Hartmann T, Bergmann C (2008) Independent inhibition of Alzheimer disease beta- and gamma-secretase cleavage by lowered cholesterol levels. J Biol Chem 283(17):11302–11311 · DOI
  14. Favari E, Zanotti I, Zimetti F, Ronda N, Bernini F, Rothblat GH (2004) Probucol inhibits ABCA1-mediated cellular lipid efflux. Arterioscler Thromb Vasc Biol 24(12):2345–2350 · DOI
  15. McNeish J, Aiello RJ, Guyot D, Turi T, Gabel C, Aldinger C, Hoppe KL, Roach ML, Royer LJ, de Wet J, Broccardo C, Chimini G, Francone OL (2000) High density lipoprotein deficiency and foam cell accumulation in mice with targeted disruption of ATP-binding cassette transporter-1. Proc Natl Acad Sci U S A 97(8):4245–4250 · DOI
  16. Triguero D, Buciak J, Pardridge WM (1990) Capillary depletion method for quantification of blood–brain barrier transport of circulating peptides and plasma proteins. J Neurochem 54(6):1882–1888 · DOI
  17. Rousselle C, Clair P, Lefauconnier JM, Kaczorek M, Scherrmann JM, Temsamani J (2000) New advances in the transport of doxorubicin through the blood–brain barrier by a peptide vector-mediated strategy. Mol Pharmacol 57(4):679–686 · DOI
  18. Dallaire L, Tremblay L, Beliveau R (1991) Purification and characterization of metabolically active capillaries of the blood–brain barrier. Biochem J 276(Pt 3):745–752 · DOI
  19. Gebhardt FM, Scott HA, Dodd PR (2010) Housekeepers for accurate transcript expression analysis in Alzheimer’s disease autopsy brain tissue. Alzheimers Dement 6(6):465–474 · DOI
  20. Weickert CS, Sheedy D, Rothmond DA, Dedova I, Fung S, Garrick T, Wong J, Harding AJ, Sivagnanansundaram S, Hunt C, Duncan C, Sundqvist N, Tsai SY, Anand J, Draganic D, Harper C (2010) Selection of reference gene expression in a schizophrenia brain cohort. Aust N Z J Psychiatry 44(1):59–70 · DOI
  21. Kim WS, Guillemin GJ, Glaros EN, Lim CK, Garner B (2006) Quantitation of ATP-binding cassette subfamily-A transporter gene expression in primary human brain cells. Neuroreport 17(9):891–896 · DOI
  22. Su YR, Linton MF, Fazio S (2002) Rapid quantification of murine ABC mRNAs by real time reverse transcriptase-polymerase chain reaction. J Lipid Res 43(12):2180–2187 · DOI
  23. Milane A, Fernandez C, Vautier S, Bensimon G, Meininger V, Farinotti R (2007) Minocycline and riluzole brain disposition: interactions with p-glycoprotein at the blood–brain barrier. J Neurochem 103(1):164–173 · DOI
  24. Dagenais C, Rousselle C, Pollack GM, Scherrmann JM (2000) Development of an in situ mouse brain perfusion model and its application to mdr1a P-glycoprotein-deficient mice. J Cereb Blood Flow Metab 20(2):381–386 · DOI
  25. Zhao R, Kalvass JC, Pollack GM (2009) Assessment of blood–brain barrier permeability using the in situ mouse brain perfusion technique. Pharm Res 26(7):1657–1664 · DOI
  26. Takasato Y, Rapoport SI, Smith QR (1984) An in situ brain perfusion technique to study cerebrovascular transport in the rat. Am J Physiol 247(3 Pt 2):H484–H493
  27. Janvilisri T, Venter H, Shahi S, Reuter G, Balakrishnan L, van Veen HW (2003) Sterol transport by the human breast cancer resistance protein (ABCG2) expressed in Lactococcus lactis. J Biol Chem 278(23):20645–20651 · DOI
  28. Koshiba S, An R, Saito H, Wakabayashi K, Tamura A, Ishikawa T (2008) Human ABC transporters ABCG2 (BCRP) and ABCG4. Xenobiotica 38(7–8):863–888 · DOI
  29. Le Goff W, Settle M, Greene DJ, Morton RE, Smith JD (2006) Reevaluation of the role of the multidrug-resistant P-glycoprotein in cellular cholesterol homeostasis. J Lipid Res 47(1):51–58 · DOI
  30. Gosselet F, Candela P, Sevin E, Berezowski V, Cecchelli R, Fenart L (2009) Transcriptional profiles of receptors and transporters involved in brain cholesterol homeostasis at the blood–brain barrier: use of an in vitro model. Brain Res 1249:34–42 · DOI
  31. Panzenboeck U, Balazs Z, Sovic A, Hrzenjak A, Levak-Frank S, Wintersperger A, Malle E, Sattler W (2002) ABCA1 and scavenger receptor class B, type I, are modulators of reverse sterol transport at an in vitro blood–brain barrier constituted of porcine brain capillary endothelial cells. J Biol Chem 277(45):42781–42789 · DOI · Paper page
  32. Cisternino S, Mercier C, Bourasset F, Roux F, Scherrmann JM (2004) Expression, up-regulation, and transport activity of the multidrug-resistance protein Abcg2 at the mouse blood–brain barrier. Cancer Res 64(9):3296–3301 · DOI
  33. Warren MS, Zerangue N, Woodford K, Roberts LM, Tate EH, Feng B, Li C, Feuerstein TJ, Gibbs J, Smith B, de Morais SM, Dower WJ, Koller KJ (2009) Comparative gene expression profiles of ABC transporters in brain microvessel endothelial cells and brain in five species including human. Pharmacol Res 59(6):404–413 · DOI
  34. Pardridge WM, Mietus LJ (1980) Palmitate and cholesterol transport through the blood–brain barrier. J Neurochem 34(2):463–466 · DOI
  35. Jurevics H, Morell P (1995) Cholesterol for synthesis of myelin is made locally, not imported into brain. J Neurochem 64(2):895–901 · DOI · Paper page
  36. Lutjohann D, Breuer O, Ahlborg G, Nennesmo I, Siden A, Diczfalusy U, Bjorkhem I (1996) Cholesterol homeostasis in human brain: evidence for an age-dependent flux of 24S-hydroxycholesterol from the brain into the circulation. Proc Natl Acad Sci USA 93(18):9799–9804 · DOI · Paper page
  37. Ohtsuki S, Ito S, Matsuda A, Hori S, Abe T, Terasaki T (2007) Brain-to-blood elimination of 24S-hydroxycholesterol from rat brain is mediated by organic anion transporting polypeptide 2 (oatp2) at the blood–brain barrier. J Neurochem 103(4):1430–1438 · DOI
  38. Cisternino S, Rousselle C, Lorico A, Rappa G, Scherrmann JM (2003) Apparent lack of Mrp1-mediated efflux at the luminal side of mouse blood–brain barrier endothelial cells. Pharm Res 20(6):904–909 · DOI
  39. Cattelotte J, Andre P, Ouellet M, Bourasset F, Scherrmann JM, Cisternino S (2008) In situ mouse carotid perfusion model: glucose and cholesterol transport in the eye and brain. J Cereb Blood Flow Metab 28(8):1449–1459 · DOI
  40. Taylor JM, Borthwick F, Bartholomew C, Graham A (2010) Overexpression of steroidogenic acute regulatory protein increases macrophage cholesterol efflux to apolipoprotein AI. Cardiovasc Res 86(3):526–534 · DOI

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Metadata

Authors
Do, T. M., Ouellet, M., Calon, F., Chimini, G., Chacun, H., Farinotti, R., & Bourasset, F.
Venue
Molecular and Cellular Biochemistry
Publisher
Springer Science and Business Media LLC
Published
2011-06-10
Volume
357
Issue
1-2
Pages
397-404
ISSN
  • 0300-8177
  • 1573-4919
DOI
10.1007/s11010-011-0910-6
Cited by
36
References
2
Language
en
Licence
www.springer.com/tdm (tdm)

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