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Calcitonin gene-related peptide stimulates proliferation and osteogenic differentiation of osteoporotic rat-derived bone mesenchymal stem cells
Abstract
Osteoporosis, a systemic bone disorder, is prevalent in postmenopausal woman. Bone mesenchymal stem cells (BMSCs), precursors of osteogenic cells, may contribute to prevention or treatment of bone frustrate in osteoporosis. Recently, two studies suggested a role of calcitonin gene-related peptide (CGRP) in promoting osteogenesis of BMSCs under physiological conditions. However, the role of CGRP on BMSCs, which are derived from osteoporotic tissues, is unclear. Here, we investigated the role of CGRP on BMSCs isolated from female osteoporotic rats. Data showed that CGRP stimulated cell proliferation and inhibited cell apoptosis for short-term culture of BMSCs. Instead, CGRP induced BMSCs differentiation into the osteoblasts and promoted formation of calcified nodules after long-term culture. Moreover, CGRP gradually up-regulated expression levels of osteoporotic differentiation-related genes including alkaline phosphatase, Collagen type I, Bmp2, Osteonectin, and Runx2 during osteogenic differentiation. In conclusion, CGRP promoted proliferation and induced osteogenic differentiation and mineralization during female osteoporotic rat-derived BMSC differentiation. These findings support a potential role of CGRP on the prevention or treatment of osteoporotic fracture.
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The doses of SP, CGRP, aprepitant, and CGRP 8-37 used were justified based on prior reports and plasma concentrations.
“With these considerations, we believe that our choice of doses are well justified.”
From Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis (2019) · cites this paper as [121]
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The Activity of Peptides of the Calcitonin Family in Bone
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Metadata
- Authors
- Liang, W., Zhuo, X., Tang, Z., Wei, X., & Li, B.
- Affiliations
- Guangxi Medical University (CN)
- Corresponding author
- Li, Bing
- Venue
- Molecular and Cellular Biochemistry
- Published
- 2015-01-07
- Volume
- 402
- Issue
- 1-2
- Pages
- 101-110
- ISSN
- 0300-8177 (ISSN-L)
- 1573-4919
- OpenAlex
- W2040863684
- Cited by
- 56
- References
- 37
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 8 · 2021: 4 · 2020: 10 · 2019: 6 · 2018: 9 · 2017: 2 · 2016: 6 · 2015: 1
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular and Cellular Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Keywords
- calcitonin gene-related peptide, bone mesenchymal stem cells, osteoporosis, osteogenic differentiation, cell proliferation, apoptosis, mineralization, osteoblasts, Runx2, BMP-2, osteoporotic fractures
- MeSH terms
- Alkaline Phosphatase, Animals, Cell Differentiation, Cells, Cultured, Female, Osteogenesis, Osteoporosis, Calcitonin Gene-Related Peptide, Up-Regulation, Rats, Sprague-Dawley, Cell Proliferation, Mesenchymal Stem Cells
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