Cited by 18Cell death mechanisms and regulationMedicinal Plant ResearchSesquiterpenes and Asteraceae Studies

Induction of Apoptosis in Human Glioma Cell Lines of Various Grades through the ROS-Mediated Mitochondrial Pathway and Caspase Activation by Rhaponticum carthamoides Transformed Root Extract

Skała, Ewa, Kowalczyk, Tomasz, Toma, Monika, Szemraj, Janusz, Radek, Maciej, Pytel, Dariusz, Wieczfinska, Joanna, Wysokińska, Halina +2 more·2018·Molecular and Cellular Biochemistry

Abstract

The present study is the first investigation of the inhibitory effect of Rhaponticum carthamoides transformed roots (TR) extract on the proliferation of grade II and III human glioma cells. TR extract showed the cytotoxic effect and inhibited the colony formation of both glioma cell lines in dose-dependent manner. The root extract induced apoptosis by increasing of the reactive oxygen species (about threefold compared to the control cells) leading to a disruption of mitochondrial membrane potential. Additionally, the mRNA levels of the apoptotic factors such as Bax, Tp53, caspase-3, and caspase-9 were observed to increase. These results indicate that the TR extract possesses anticancer activity by inhibiting glioma cell proliferation and inducing apoptotic cell death, and may be used as a promising anticancer agent.

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Crossref · 10.1007/s11010-017-3254-z

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Authors
Skała, E., Kowalczyk, T., Toma, M., Szemraj, J., Radek, M., Pytel, D., Wieczfinska, J., Wysokińska, H., Śliwiński, T., & Sitarek, P.
Affiliations
  • Medical University of Lodz (PL)
  • University of Łódź (PL)
  • Medical University of South Carolina (US)
Corresponding author
Skała, Ewa
Venue
Molecular and Cellular Biochemistry
Published
2017-12-14
Volume
445
Issue
1-2
Pages
89-97
ISSN
  • 0300-8177 (ISSN-L)
  • 1573-4919
OpenAlex
W2771318378
Cited by
18
References
31
Citations by year
2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 4
FWCI
1.01
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular and Cellular Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
Keywords
Rhaponticum carthamoides, glioma, apoptosis, reactive oxygen species, mitochondrial pathway, caspase-3, caspase-9, Bax, p53, mitochondrial membrane potential, anticancer activity, cytotoxicity, cell proliferation inhibition
MeSH terms
Antineoplastic Agents, Phytogenic, Brain Neoplasms, Enzyme Activation, Glioma, Humans, Middle Aged, Plant Extracts, Tumor Suppressor Protein p53, Reactive Oxygen Species, Plant Roots, Leuzea, Cell Line, Tumor, Cell Proliferation, bcl-2-Associated X Protein, Membrane Potential, Mitochondrial, Caspase 3, Caspase 9

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