Induction of Apoptosis in Human Glioma Cell Lines of Various Grades through the ROS-Mediated Mitochondrial Pathway and Caspase Activation by Rhaponticum carthamoides Transformed Root Extract
Abstract
The present study is the first investigation of the inhibitory effect of Rhaponticum carthamoides transformed roots (TR) extract on the proliferation of grade II and III human glioma cells. TR extract showed the cytotoxic effect and inhibited the colony formation of both glioma cell lines in dose-dependent manner. The root extract induced apoptosis by increasing of the reactive oxygen species (about threefold compared to the control cells) leading to a disruption of mitochondrial membrane potential. Additionally, the mRNA levels of the apoptotic factors such as Bax, Tp53, caspase-3, and caspase-9 were observed to increase. These results indicate that the TR extract possesses anticancer activity by inhibiting glioma cell proliferation and inducing apoptotic cell death, and may be used as a promising anticancer agent.
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Investigations into Rhaponticum carthamoides revealed that its extracts are low in toxicity.
“The investigations into R. carthamoides revealed that its extracts are low in toxicity [69,121,122,123,124,125,126,127,128,129,130,131,132].”
From Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition (2024) · cites this paper as [121]
Extracts from Rhaponticum carthamoides show antibacterial, cytotoxic, anti-adipogenic, and antioxidant activities.
“Extracts from R. carthamoides show a variety of activities, including antibacterial, cytotoxic, anti-adipogenic, and antioxidant [69,121,122,123,124,125,126,127,128,129,130,131,132].”
From Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition (2024) · cites this paper as [121]
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Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition
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- Authors
- Skała, E., Kowalczyk, T., Toma, M., Szemraj, J., Radek, M., Pytel, D., Wieczfinska, J., Wysokińska, H., Śliwiński, T., & Sitarek, P.
- Affiliations
- Medical University of Lodz (PL)
- University of Łódź (PL)
- Medical University of South Carolina (US)
- Corresponding author
- Skała, Ewa
- ORCID
- Venue
- Molecular and Cellular Biochemistry
- Published
- 2017-12-14
- Volume
- 445
- Issue
- 1-2
- Pages
- 89-97
- ISSN
- 0300-8177 (ISSN-L)
- 1573-4919
- OpenAlex
- W2771318378
- Cited by
- 18
- References
- 31
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 4
- FWCI
- 1.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular and Cellular Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
- Keywords
- Rhaponticum carthamoides, glioma, apoptosis, reactive oxygen species, mitochondrial pathway, caspase-3, caspase-9, Bax, p53, mitochondrial membrane potential, anticancer activity, cytotoxicity, cell proliferation inhibition
- MeSH terms
- Antineoplastic Agents, Phytogenic, Brain Neoplasms, Enzyme Activation, Glioma, Humans, Middle Aged, Plant Extracts, Tumor Suppressor Protein p53, Reactive Oxygen Species, Plant Roots, Leuzea, Cell Line, Tumor, Cell Proliferation, bcl-2-Associated X Protein, Membrane Potential, Mitochondrial, Caspase 3, Caspase 9
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