Virus-induced silencing of genes encoding LEA protein in Tibetan hulless barley (Hordeum vulgare ssp. vulgare) and their relationship to drought tolerance
Abstract
Expression of the late embryogenesis abundant (LEA) gene is usually associated with plant response to dehydration. In this study, a drought-tolerant genotype was screened from 48 accessions of Tibetan hulless barley (Hordeum vulgare ssp. vulgare). By using virus-induced gene silencing, the influence of two LEA genes (HVA1 and Dhn6) on drought tolerance of Tibetan hulless barley was investigated. Results of quantitative real-time PCR indicated that the relative expression levels of HVA1 and Dhn6 in silenced plants were significantly reduced compared with control plants. Both HVA1-silenced and Dhn6-silenced plants showed a consequently lower survival rate than control plants under drought stress. However, only HVA1-silenced plants exhibited a significantly higher water loss rate (WLR). These results suggested that HVA1 and Dhn6 might participate in adaptive responses to water deficit in different ways. Vegetative growth of HVA1-silenced plants was significantly retarded even under optimal growth conditions, and their biomass accumulation was also much lower than that of the controls. These results indicate that HVA1 might play a role in vegetative growth of Tibetan hulless barley.
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VIGS has been used to functionally analyse the Triticum aestivum enhanced response to abscisic acid 1 (TaEra1) and basic transcription factor 3 (TaBTF3) genes in wheat, and inwardly rectifying potassium (K+) channels HvAKT1 and HvAKT2, HVA1, and Hv dehydrin 6 (HvDhn6) genes in barley for drought tolerance.
“VIGS has been used to functionally analyse the Triticum aestivum enhanced response to abscisic acid 1 (TaEra1) and basic transcription factor 3 (TaBTF3) genes in wheat, and inwardly rectifying potassium (K + ) channels HvAKT1 and HvAKT2, HVA1, and Hv dehydrin 6 (HvDhn6) genes in barley for drought tolerance (Liang et al., 2012;Kang et al., 2013;Manmathan et al., 2013;Feng et al., 2020a).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Liang et al., 2012
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- Authors
- Liang, J., Deng, G., Long, H., Pan, Z., Wang, C., Cai, P., Xu, D., Nima, Z.-X., & Yu, M.
- Affiliations
- Chinese Academy of Sciences (CN)
- China West Normal University (CN)
- Tibet Academy of Agricultural and Animal Husbandry Sciences (CN)
- Corresponding author
- Long, Hai
- Yu, Maoqun
- Venue
- Molecular Breeding
- Published
- 2011-09-22
- Volume
- 30
- Issue
- 1
- Pages
- 441-451
- ISSN
- 1380-3743 (ISSN-L)
- 1572-9788
- OpenAlex
- W1991019868
- Cited by
- 59
- References
- 60
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 5 · 2023: 2 · 2022: 12 · 2021: 5 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 3 · 2016: 7 · 2015: 4 · 2014: 3 · 2013: 4 · 2012: 1
- FWCI
- 3.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular Breeding (publishedVersion)
- Funders
- Chengdu Institute of Biology
- Institute of Genetics
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetic and Environmental Crop Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetics and Plant Breeding
- Keywords
- late embryogenesis abundant proteins, LEA genes, drought tolerance, virus-induced gene silencing, barley, dehydration stress, water loss rate, vegetative growth, biomass accumulation, drought-tolerant genotypes
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