Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene
Abstract
The ABA-responsive barley gene HVA1, a member of group 3 late embryogenesis abundant (LEA) protein genes, was introduced into spring wheat (Triticum aestivum L.) cv. Hi-Line using the biolistic bombardment method. High levels of expression of the HVA1 gene, regulated by the maize ubi1 promoter, were observed in leaves and roots of independent transgenic wheat plants and were inherited by offspring generations. T(3) progenies of four selected transgenic wheat lines were tested under greenhouse conditions for tolerance of soil water deficit. Potted plants were grown under moderate water deficit and well-watered conditions, respectively. Two homozygous and one heterozygous transgenic lines expressing the HVA1 gene had significantly (P<0.01) higher water use efficiency values, 0.66-0.68 g kg(-1), as compared to 0.57 and 0.53 g kg(-1), respectively, for the non-expressing transgenic and non-transgenic controls under moderate water deficit conditions. The two homozygous transgenic plant lines also had significantly greater total dry mass, root fresh and dry weights, and shoot dry weight compared to the two controls under soil water deficit conditions. Results of this study indicate that growth characteristics were improved in transgenic wheat plants constitutively expressing the barley HVA1 gene in response to soil water deficit.
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Several barley genes have been exploited to address different stress conditions, including HVA1 for drought, HKT1;1 for salinity, and ARE1 for nitrogen use efficiency.
“Several barley genes have been exploited to address different stress conditions (Zhou et al., 2016;Hazzouri et al., 2018;Karunarathne et al., 2020;Mwando et al., 2020), including Hordeum vulgare aleurone 1 (HVA1) for drought, high-affinity K + transporter 1;1 (HKT1;1) for salinity, and abnormal cytokinin response 1 repressor 1 (ARE1) for nitrogen use efficiency (NUE) (Sivamani et al., 2000;Han et”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Sivamani et al., 2000
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“…et al., 2020), including Hordeum vulgare aleurone 1 ( HVA1 ) for drought, high-affinity K + trans‐ porter 1;1 ( HKT1;1 ) for salinity, and abnormal cytoki‐ nin response 1 repressor 1 ( ARE1 ) for nitrogen use efficiency (NUE) (Sivamani et al., 2000; Han et al., 2018; Karunarathne et al., 2022).”
- Authors
- Sivamani, E., Bahieldin, A., Wraith, J. M., Al-Niemi, T., Dyer, W. E., Ho, T.-H. D., & Qu, R.
- Affiliations
- Ain Shams University (EG)
- Montana State University (US)
- Washington University in St. Louis (US)
- Corresponding author
- Bahieldin, Ahmed
- Venue
- Plant Science
- Publisher
- Elsevier BV
- Published
- 2000-06
- Volume
- 155
- Issue
- 1
- Pages
- 1-9
- ISSN
- 0168-9452 (ISSN-L)
- OpenAlex
- W2124362687
- Cited by
- 500
- References
- 36
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 12 · 2023: 18 · 2022: 26 · 2021: 28 · 2020: 18 · 2019: 18 · 2018: 16 · 2017: 18 · 2016: 33 · 2015: 20 · 2014: 18 · 2013: 34 · 2012: 25
- FWCI
- 14.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Plant Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Montana Wheat and Barley Committee
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetics and Plant Breeding
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Keywords
- late embryogenesis abundant proteins, transgenic wheat, soil water deficit, drought tolerance, water use efficiency, biomass productivity, particle bombardment, constitutive expression, root growth, abiotic stress tolerance
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