Quantitative proteomics to study the response of wheat to contrasting fertilisation regimes
Abstract
Negative environmental impacts from mineral fertilisers and pesticides used in conventional cropping have raised concern over the sustainability of arable crop production. Organic cropping uses alternatives that avoid many of these negative environmental effects; however, crop yields can be significantly reduced, possibly due to a lower proportion of plant-available nutrients. To gain insights into the molecular effects of organic compared to conventional cropping systems on plant utilisation of nutrients, we used proteomics to analyse winter wheat (Triticum aestivum). Our aim was to investigate the effects of contrasting fertility management and crop protection regimes in organic and conventional cropping systems on the wheat flag leaf proteome and the association between the proteome and physiological traits. Wheat flag leaves were flash-frozen, lyophilised and milled prior to protein extraction (TCA/acetone) and analysed using 2D gel electrophoresis and MALDI-TOF MS. The abundance of 111 protein spots varied significantly between fertilisation regimes. Flag leaf N and P composition were significant drivers of differences in protein spot abundance, including major proteins involved in nitrogen remobilisation, photosynthesis, metabolism and stress response. These results indicate that molecular-based mechanisms are involved in the effect of contrasting cropping systems on nutrient utilisation and wheat grain yield. Using a functional genomics approach, we were able to identify proteins that are linked to causal genes, enabling the potential development of functional molecular markers for crop improvement in nutrient use efficiency.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A switch from pesticide-based conventional to organic crop protection protocols has a significant but more limited effect than fertilisation regimens on gene and protein expression patterns.
“Also, a switch from pesticide-based conventional to organic crop protection protocols has been shown to have a significant, but more limited effect than fertilisation regimens”
The switch from mineral to organic fertilisers results in significant differences in gene and protein expression patterns and, as a result, in secondary metabolite profiles; approximately 10% of proteins have been found to be either up- or down-regulated in response to contrasting fertiliser inputs in potato and wheat.
“Recent studies have shown that the switch from mineral to organic fertilisers results in significant differences in gene and protein expression patterns and, as a result, in secondary metabolite profiles; for example, approximately 10 % of proteins have been found to be either up- or down-regulated in response to contrasting fertiliser inputs in potato and wheat( 10 – 15 ).”
There are statistically significant interactions between fertilisation and crop protection protocols with respect to gene and protein expression patterns.
“there were some statistically significant interactions between fertilisation and crop protection protocols with respect to gene and protein expression pattern”
Both genetic and environmental/agronomic factors are known to affect the concentrations of N, [nitrate/nitrite], proteins, sugars, antioxidants/(poly)phenolics, Cd and pesticides in crops.
“This is not surprising as both genetic and environmental/agronomic factors are known to affect the concentrations of N, , , proteins, sugars, antioxidants/(poly)phenolics, Cd and pesticides in crops( 7 , 9 – 12 , 20 , 47 – 52 , 62 ).”
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Nitrogen in agriculture: balancing the cost of an essential resource
Robertson, G. P., & Vitousek, P. M. (2009) · Annual Review of Environment and Resources · doi:10.1146/annurev.environ.032108.105046 · Cited by 1,177 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Barański, M., Średnicka-Tober, D., Volakakis, N., Seal, C., Sanderson, R., Stewart, G. B., Benbrook, C., Biavati, B., Markellou, E., Giotis, C., et al. (2014) · British Journal of Nutrition · doi:10.1017/s0007114514001366 · Cited by 684 · see citing works
Self-citationBilsborrow, P., Cooper, J., Tétard-Jones, C., Średnicka-Tober, D., Barański, M., Eyre, M., Schmidt, C., Shotton, P., Volakakis, N., Cakmak, I., et al. (2013) · European Journal of Agronomy · doi:10.1016/j.eja.2013.06.003 · Cited by 97 · see citing works
Self-citationTétard-Jones, C., Edwards, M., Rempelos, L., Gatehouse, A., Eyre, M., Wilcockson, S., & Leifert, C. (2013) · Agronomy · doi:10.3390/agronomy3010059 · Cited by 14 · see citing works
Self-citation
- Authors
- Tétard-Jones, C., Shotton, P. N., Rempelos, L., Cooper, J., Eyre, M., Orr, C. H., Leifert, C., & Gatehouse, A. M. R.
- Affiliations
- Newcastle University (GB)
- Northumbria University (GB)
- Corresponding author
- Gatehouse, Angharad M. R.
- Venue
- Molecular Breeding
- Published
- 2012-11-03
- Volume
- 31
- Issue
- 2
- Pages
- 379-393
- ISSN
- 1380-3743 (ISSN-L)
- 1572-9788
- OpenAlex
- W2044659218
- Cited by
- 25
- References
- 1
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 2 · 2018: 4 · 2017: 4 · 2016: 2 · 2014: 5 · 2013: 2
- FWCI
- 3.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular Breeding (publishedVersion)
- Teesside University Research Portal (Teesside University) (submittedVersion)
- TeesRep (Teesside University) (submittedVersion)
- Northumbria Research Link (Northumbria University) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Agricultural Science and Fertilization
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- wheat, organic fertilization, nitrogen remobilization, nutrient use efficiency, photosynthesis, stress response, grain yield, molecular markers
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
