The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Extrinsic Fluorescent Dyes as Tools for Protein Characterization
Open accessAbstract
Noncovalent, extrinsic fluorescent dyes are applied in various fields of protein analysis, e.g. to characterize folding intermediates, measure surface hydrophobicity, and detect aggregation or fibrillation. The main underlying mechanisms, which explain the fluorescence properties of many extrinsic dyes, are solvent relaxation processes and (twisted) intramolecular charge transfer reactions, which are affected by the environment and by interactions of the dyes with proteins. In recent time, the use of extrinsic fluorescent dyes such as ANS, Bis-ANS, Nile Red, Thioflavin T and others has increased, because of their versatility, sensitivity and suitability for high-throughput screening. The intention of this review is to give an overview of available extrinsic dyes, explain their spectral properties, and show illustrative examples of their various applications in protein characterization.
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Cites
[18] Quantification of β-sheet amyloid fibril structures with thioflavin T
LeVine, H. (1999) · Methods in Enzymology · doi:10.1016/s0076-6879(99)09020-5 · Cited by 1,365 · cited 1×
“for quantification of amyloid fibrils in vitro in presence of amyloid precursor proteins and amorphous aggregates (65).”
BackgroundEffects of protein aggregates: An immunologic perspective
Rosenberg, A. S. (2006) · The AAPS Journal · doi:10.1208/aapsj080359 · Cited by 1,355
Spectrofluorometric studies of the lipid probe, nile red.
Greenspan, P., & Fowler, S. D. (1985) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34307-8 · Cited by 980
Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces
Sackett, D. L., & Wolff, J. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90157-6 · Cited by 591
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A Reversible Fluorescent Probe for Real‐Time Quantitative Monitoring of Cellular Glutathione
Liu, Z., Zhou, X., Miao, Y., Hu, Y., Kwon, N., Wu, X., & Yoon, J. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201702114 · Cited by 312
Rodríguez-Rodríguez, C., Telpoukhovskaia, M., & Orvig, C. (2012) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2012.03.008 · Cited by 151
Autofluorescence of atmospheric bioaerosols: spectral fingerprints and taxonomic trends of pollen
Pöhlker, C., Huffman, J. A., Förster, J.-D., & Pöschl, U. (2013) · Atmospheric Measurement Techniques · doi:10.5194/amt-6-3369-2013 · Cited by 128
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Tursi, S. A., Lee, E. Y., Medeiros, N. J., Lee, M. H., Nicastro, L. K., Buttaro, B., Gallucci, S., Wilson, R. P., Wong, G. C. L., & Tükel, Ç. (2017) · PLOS Pathogens · doi:10.1371/journal.ppat.1006315 · Cited by 115
Metadata
- Authors
- Hawe, A., Sutter, M., & Jiskoot, W.
- Venue
- Pharmaceutical Research
- Published
- 2008-01-03
- Volume
- 25
- Issue
- 7
- Pages
- 1487-1499
- ISSN
- 0724-8741 (ISSN-L)
- 1573-904X
- OpenAlex
- W2108305553
- Cited by
- 1,199
- References
- 4
- FWCI
- 17.00
- Type
- journal article
- Language
- en
- Open access
- hybrid
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