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Cited by 128Allergic Rhinitis and SensitizationAtmospheric chemistry and aerosolsIndoor Air Quality and Microbial Exposure

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Autofluorescence of atmospheric bioaerosols: spectral fingerprints and taxonomic trends of pollen

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Pöhlker, C., Huffman, J. A., Förster, J.-D., Pöschl, U.·2013·Atmospheric Measurement Techniques
DOI 10.5194/amt-6-3369-2013

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Abstract

Abstract. Primary biological aerosol particles (PBAP) are important factors in atmospheric cycling, climate, and public health. Pollen is a major fraction of PBAP and is receiving increasing attention due to its high allergenic potential and the associated impacts on personal life quality and economy. Recently, autofluorescence-based techniques have proven to be valuable tools for real time, in situ quantification and classification of PBAP. First studies suggest that the autofluorescence of pollen may be sufficiently selective to be utilized for an automated and real-time monitoring of pollen in ambient air. However, the degree of selectivity autofluorescence can provide is still in question and actively debated. This study addresses the origin, properties, and selectivity of autofluorescence from natural pollen by fluorescence microscopy and spectroscopy measurements along with a systematic synthesis of related literature. We show that dry pollen reveals characteristic and reproducible autofluorescence signatures which are shaped by cell wall associated fluorophores, such as phenolic compounds and carotenoid pigments. In addition, fluorescence signals from proteins and chlorophyll a were observed in some species. The abundance and intensity of the individual fluorescence signals show certain taxonomic trends and allow systematic differentiation from bacteria and fungal spores due to the lack of proteins on the grain surface. Principal component analysis was used to explore the discrimination potential of pollen autofluorescence, in combination with size and shape, revealing a differentiation of pollen on family level. Our results help explore the levels of selectivity that autofluorescence-based techniques can provide to PBAP analysis and will support the development and application of autofluorescence-based detectors for monitoring of allergenic pollen in the atmosphere.

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Metadata

Authors
Pöhlker, C., Huffman, J. A., Förster, J.-D., & Pöschl, U.
Affiliations
  • Max Planck Institute for Chemistry (DE)
  • University of Denver (US)
Corresponding author
Huffman, J. A.
ORCID
Venue
Atmospheric Measurement Techniques
Publisher
Copernicus GmbH
Published
2013-12-09
Volume
6
Issue
12
Pages
3369-3392
ISSN
  • 1867-1381 (ISSN-L)
  • 1867-8548
DOI
10.5194/amt-6-3369-2013
OpenAlex
W2011660542
Cited by
128
References
2
Citations by year
2026: 11 · 2025: 12 · 2024: 11 · 2023: 11 · 2022: 16 · 2021: 12 · 2020: 9 · 2019: 10 · 2018: 11 · 2017: 6 · 2016: 10 · 2015: 2 · 2014: 4 · 2013: 3
FWCI
6.13
Type
journal article
Language
en
Open access
Licence
Locations
  • Atmospheric measurement techniques (publishedVersion, cc-by)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • Max Planck Digital Library (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Funders
  • University of Denver
  • Johannes Gutenberg-Universität Mainz
  • Max-Planck-Gesellschaft
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
  • Physical Sciences › Earth and Planetary Sciences › Atmospheric Science › Atmospheric chemistry and aerosols
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Indoor Air Quality and Microbial Exposure
Keywords
pollen, autofluorescence, bioaerosols, pollen classification, pollen taxonomy, phenolic compounds, carotenoids, chlorophyll a, protein fluorescence, bacterial spores, fungal spores, fluorescence spectroscopy, fluorescence microscopy, particle size and shape

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