The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants
Open accessAbstract
Transcription activator-like effector nucleases (TALENs) enable targeted mutagenesis in a variety of organisms. The primary advantage of TALENs over other sequence-specific nucleases, namely zinc finger nucleases and meganucleases, lies in their ease of assembly, reliability of function, and their broad targeting range. Here we report the assembly of several TALENs for a specific genomic locus in barley. The cleavage activity of individual TALENs was first tested in vivo using a yeast-based, single-strand annealing assay. The most efficient TALEN was then selected for barley transformation. Analysis of the resulting transformants showed that TALEN-induced double strand breaks led to the introduction of short deletions at the target site. Additional analysis revealed that each barley transformant contained a range of different mutations, indicating that mutations occurred independently in different cells.
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Cites
A physical, genetic and functional sequence assembly of the barley genome
The International Barley Genome Sequencing Consortium (2012) · Nature · doi:10.1038/nature11543 · Cited by 1,456
An improved zinc-finger nuclease architecture for highly specific genome editing
Miller, J. C., Holmes, M. C., Wang, J., Guschin, D. Y., Lee, Y.-L., Rupniewski, I., Beausejour, C. M., Waite, A. J., Wang, N. S., Kim, K. A., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1319 · Cited by 1,051
TAL effectors: customizable proteins for DNA targeting
Bogdanove, A. J., & Voytas, D. F. (2011) · Science · doi:10.1126/science.1204094 · Cited by 984
Self-citationEnhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures
Doyon, Y., Vo, T. D., Mendel, M. C., Greenberg, S. G., Wang, J., Xia, D. F., Miller, J. C., Urnov, F. D., Gregory, P. D., & Holmes, M. C. (2011) · Nature Methods · doi:10.1038/nmeth.1539 · Cited by 402
Cited by
Wang, F., Wang, C., Liu, P., Lei, C., Hao, W., Gao, Y., Liu, Y.-G., & Zhao, K. (2016) · PLOS ONE · doi:10.1371/journal.pone.0154027 · Cited by 819
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation
Lowder, L. G., Zhang, D., Baltes, N. J., Paul, J. W., Tang, X., Zheng, X., Voytas, D. F., Hsieh, T.-F., Zhang, Y., & Qi, Y. (2015) · Plant Physiology · doi:10.1104/pp.15.00636 · Cited by 707
Self-citationTargeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System
Liang, Z., Zhang, K., Chen, K., & Gao, C. (2014) · Journal of Genetics and Genomics · doi:10.1016/j.jgg.2013.12.001 · Cited by 658
Genome Engineering with Targetable Nucleases
Carroll, D. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035418 · Cited by 568
Precision genome engineering and agriculture: opportunities and regulatory challenges
Voytas, D. F., & Gao, C. (2014) · PLoS Biology · doi:10.1371/journal.pbio.1001877 · Cited by 458
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Metadata
- Authors
- Wendt, T., Holm, P. B., Starker, C. G., Christian, M., Voytas, D. F., Brinch-Pedersen, H., & Holme, I. B.
- Venue
- Plant Molecular Biology
- Published
- 2013-05-21
- Volume
- 83
- Issue
- 3
- Pages
- 279-285
- ISSN
- 0167-4412 (ISSN-L)
- 1573-5028
- OpenAlex
- W2095307330
- Cited by
- 191
- References
- 4
- FWCI
- 11.43
- Type
- journal article
- Language
- en
- Open access
- green
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- Wendt, T., Holm, P. B., Starker, C. G., Christian, M., Voytas, D. F., Brinch-Pedersen, H., & Holme, I. B. (2013). TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants. Plant Molecular Biology. https://doi.org/10.1007/s11103-013-0078-4
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