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Ultrafast transient absorption spectroscopy: principles and application to photosynthetic systems

Open access
Berera, Rudi, van Grondelle, Rienk, Kennis, John T. M.·2009·Photosynthesis Research

Abstract

The photophysical and photochemical reactions, after light absorption by a photosynthetic pigment-protein complex, are among the fastest events in biology, taking place on timescales ranging from tens of femtoseconds to a few nanoseconds. The advent of ultrafast laser systems that produce pulses with femtosecond duration opened up a new area of research and enabled investigation of these photophysical and photochemical reactions in real time. Here, we provide a basic description of the ultrafast transient absorption technique, the laser and wavelength-conversion equipment, the transient absorption setup, and the collection of transient absorption data. Recent applications of ultrafast transient absorption spectroscopy on systems with increasing degree of complexity, from biomimetic light-harvesting systems to natural light-harvesting antennas, are presented. In particular, we will discuss, in this educational review, how a molecular understanding of the light-harvesting and photoprotective functions of carotenoids in photosynthesis is accomplished through the application of ultrafast transient absorption spectroscopy.

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Metadata

Authors
Berera, R., van Grondelle, R., & Kennis, J. T. M.
Venue
Photosynthesis Research
Published
2009-07-04
Volume
101
Issue
2-3
Pages
105-118
ISSN
  • 0166-8595 (ISSN-L)
  • 1573-5079
OpenAlex
W2021884442
Cited by
894
References
64
FWCI
6.32
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Ultrafast transient absorption spectroscopy: principles and application to photosynthetic systems. https://app.cochat.ai/papers/10.1007/s11120-009-9454-y
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Berera, R., van Grondelle, R., & Kennis, J. T. M. (2009). Ultrafast transient absorption spectroscopy: principles and application to photosynthetic systems. Photosynthesis Research. https://doi.org/10.1007/s11120-009-9454-y

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