Transgenic maize plants expressing a fungal phytase gene
Abstract
Maize seeds are the major ingredient of commercial pig and poultry feed. Phosphorus in maize seeds exists predominantly in the form of phytate. Phytate phosphorus is not available to monogastric animals and phosphate supplementation is required for optimal animal growth. Undigested phytate in animal manure is considered a major source of phosphorus pollution to the environment from agricultural production. Microbial phytase produced by fermentation as a feed additive is widely used to manage the nutritional and environmental problems caused by phytate, but the approach is associated with production costs for the enzyme and requirement of special cares in feed processing and diet formulation. An alternative approach would be to produce plant seeds that contain high phytase activities. We have over-expressed Aspergillus niger phyA2 gene in maize seeds using a construct driven by the maize embryo-specific globulin-1 promoter. Low-copy-number transgenic lines with simple integration patterns were identified. Western-blot analysis showed that the maize-expressed phytase protein was smaller than that expressed in yeast, apparently due to different glycosylation. Phytase activity in transgenic maize seeds reached approximately 2,200 units per kg seed, about a 50-fold increase compared to non-transgenic maize seeds. The phytase expression was stable across four generations. The transgenic seeds germinated normally. Our results show that the phytase expression lines can be used for development of new maize hybrids to improve phosphorus availability and reduce the impact of animal production on the environment.
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Genetic engineering offers new possibilities for approaching both objectives, including improving the nutritional value of feed, lowering N and P pollution through altered crop composition, and reducing manure excretion through a higher NE value.
“Genetic engineering offers new possibilities for approaching both of these objectives, including improving the nutritional value of feed (e.g., AA content; Huang et al., 2006), lowering N and P pollution through altered crop composition (e.g., low phytate; Chen et al., 2008), and reducing manure excretion through a higher NE value (e.g., reduced lignin; Jung et al., 2012).”
From Prevalence and impacts of genetically engineered feedstuffs on livestock populations1 (2014) · cites this paper as Chen et al., 2008
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Prevalence and impacts of genetically engineered feedstuffs on livestock populations1
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- Authors
- Chen, R., Xue, G., Chen, P., Yao, B., Yang, W., Ma, Q., Fan, Y., Zhao, Z., Tarczynski, M. C., & Shi, J.
- Affiliations
- Chinese Academy of Agricultural Sciences (CN)
- Pioneer Hi-Bred (US)
- Corresponding author
- Fan, Yunliu
- Venue
- Transgenic Research
- Published
- 2007-10-12
- Volume
- 17
- Issue
- 4
- Pages
- 633-643
- ISSN
- 0962-8819 (ISSN-L)
- 1573-9368
- OpenAlex
- W2145092790
- Cited by
- 196
- References
- 49
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 6 · 2023: 5 · 2022: 18 · 2021: 11 · 2020: 16 · 2019: 11 · 2018: 11 · 2017: 11 · 2016: 11 · 2015: 14 · 2014: 18 · 2013: 23 · 2012: 9
- FWCI
- 5.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Transgenic Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytase and its Applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- phytase, transgenic maize, phytate phosphorus, maize seeds, transgene integration, glycosylation, phosphorus availability, animal feed, phosphorus pollution, transgene stability
- MeSH terms
- Aspergillus niger, Zea mays, Globulins, Phosphorus, 6-Phytase, Phytic Acid, Plasmids, Promoter Regions, Genetic, Seeds, Transformation, Genetic, Blotting, Western, Plants, Genetically Modified
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