Impact of culture conditions on the chlorophyll content of microalgae for biotechnological applications
Abstract
Chlorophyll is a commercially important natural green pigment responsible for the absorption of light energy and its conversion into chemical energy via photosynthesis in plants and algae. This bioactive compound is widely used in the food, cosmetic, and pharmaceutical industries. Chlorophyll has been consumed for health benefits as a nutraceutical agent with antioxidant, anti-inflammatory, antimutagenic, and antimicrobial properties. Microalgae are photosynthesizing microorganisms which can be extracted for several high-value bioproducts in the biotechnology industry. These microorganisms are highly efficient at adapting to physicochemical variations in the local environment. This allows optimization of culture conditions for inducing microalgal growth and biomass production as well as for changing their biochemical composition. The modulation of microalgal culture under controlled conditions has been proposed to maximize chlorophyll accumulation. Strategies reported in the literature to promote the chlorophyll content in microalgae include variation in light intensity, culture agitation, and changes in temperature and nutrient availability. These factors affect chlorophyll concentration in a species-specific manner; therefore, optimization of culture conditions has become an essential requirement. This paper provides an overview of the current knowledge on the effects of key environmental factors on microalgal chlorophyll accumulation, focusing on small-scale laboratory exp…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The application of OD 680 is constrained to the period of logarithmic or exponential growth.
“However, its application is constrained to the period of logarithmic or exponential growth”
From Quantitative Prediction of Spirulina platensis Biomass Using UV-Vis Spectrophotometry (2026) · cites this paper as Ferreira & Sant, 2017
Cites
Fan, J., Cui, Y., Wan, M., Wang, W., & Li, Y. (2014) · Biotechnology for Biofuels · doi:10.1186/1754-6834-7-17 · Cited by 341 · see citing works
Phototrophic pigment production with microalgae: biological constraints and opportunities
Mulders, K. J. M., Lamers, P. P., Martens, D. E., & Wijffels, R. H. (2014) · Journal of Phycology · doi:10.1111/jpy.12173 · Cited by 302 · see citing works
Novel chlorophylls and new directions in photosynthesis research
Li, Y., & Chen, M. (2015) · Functional Plant Biology · doi:10.1071/fp14350 · Cited by 96 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
The Potential and Challenge of Microalgae as Promising Future Food Sources
Chen, C., Tang, T., Shi, Q., Zhou, Z., & Fan, J. (2022) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2022.06.016 · Cited by 229 · see citing works
Tzima, S., Georgiopoulou, I., Louli, V., & Magoulas, K. (2023) · Molecules · doi:10.3390/molecules28031410 · Cited by 135 · see citing works
Shekh, A., Sharma, A., Schenk, P. M., Kumar, G., & Mudliar, S. (2022) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.6902 · Cited by 90 · see citing works
The Effect of Variable Light Source and Light Intensity on the Growth of Three Algal Species
Bialevich, V., Zachleder, V., & Bišová, K. (2022) · Cells · doi:10.3390/cells11081293 · Cited by 87 · see citing works
Schagerl, M., Siedler, R., Konopáčová, E., & Ali, S. S. (2022) · Cells · doi:10.3390/cells11152455 · Cited by 82 · see citing works
Influence of Nutrient-Stress Conditions on Chlorella vulgaris Biomass Production and Lipid Content
Ratomski, P., & Hawrot-Paw, M. (2021) · Catalysts · doi:10.3390/catal11050573 · Cited by 75 · see citing works
Rosero-Chasoy, G., Rodríguez-Jasso, R. M., Aguilar, C. N., Buitrón, G., Chairez, I., & Ruiz, H. A. (2022) · Bioresource Technology · doi:10.1016/j.biortech.2021.126456 · Cited by 44 · see citing works
Microalgal kinetics—A guideline for photobioreactor design and process development
Schediwy, K., Trautmann, A., Steinweg, C., & Posten, C. (2019) · Engineering in Life Sciences · doi:10.1002/elsc.201900107 · Cited by 42 · see citing works
Bioactive compounds from micro-algae and its application in foods: A review
Siddhnath, Surasani, V. K. R., Singh, A., Singh, S. M., Hauzoukim, Murthy, L. N., & Baraiya, K. G. (2024) · Discover Food · doi:10.1007/s44187-024-00096-6 · Cited by 42 · see citing works
Tetraedron minimum, First Reported Member of Hydrodictyaceae to Accumulate Secondary Carotenoids
Doppler, P., Kornpointner, C., Halbwirth, H., Remias, D., & Spadiut, O. (2021) · Life · doi:10.3390/life11020107 · Cited by 17 · see citing works
Quantitative Prediction of Spirulina platensis Biomass Using UV-Vis Spectrophotometry
Lestari, F. S., Zulys, A., & Satya, A. (2026) · LIMNOTEK Perairan Darat Tropis di Indonesia · doi:10.55981/limnotek.2026.14459
“However, its application is constrained to the period of logarithmic or exponential growth (Ferreira & Sant, 2017; Nielsen & Hansen, 2019).”
- Authors
- da Silva Ferreira, V., & Sant’Anna, C.
- Affiliations
- Instituto Nacional de Metrologia, Qualidade e Tecnologia (BR)
- Corresponding author
- Sant’Anna, Celso
- ORCID
- Sant’Anna, Celso
- Venue
- World Journal of Microbiology and Biotechnology
- Published
- 2016-12-01
- Volume
- 33
- Issue
- 1
- Pages
- 20-20
- ISSN
- 0959-3993 (ISSN-L)
- 1573-0972
- OpenAlex
- W2557369384
- Cited by
- 289
- References
- 3
- Citations by year
- 2026: 20 · 2025: 33 · 2024: 31 · 2023: 42 · 2022: 49 · 2021: 40 · 2020: 35 · 2019: 25 · 2018: 7 · 2017: 6
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- World Journal of Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- chlorophyll accumulation, microalgae, culture conditions, light intensity, temperature, nutrient availability, microalgae cultivation, nutraceuticals, antioxidant activity, bioproducts
- MeSH terms
- Biotechnology, Chlorophyll, Light, Photosynthesis, Temperature, Biomass, Microalgae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
