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Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites
Open accessAbstract
Immunoassays are antibody-based analytical methods for quantitative/qualitative analysis. Since the principle of immunoassays is based on specific antigen-antibody reaction, the assays have been utilized worldwide for diagnosis, pharmacokinetic studies by drug monitoring, and the quality control of commercially available products. Berson and Yalow were the first to develop an immunoassay, known as radioimmunoassay (RIA), for detecting endogenous plasma insulin [1], a development for which Yalow was awarded the Nobel Prize in Physiology or Medicine in 1977. Even today, after half a century, immunoassays are widely utilized with some modifications from the originally proposed system, e.g., radioisotopes have been replaced with enzymes because of safety concerns regarding the use of radioactivity, which is referred to as enzyme immunoassay/enzyme-linked immunosorbent assay (ELISA). In addition, progress has been made in ELISA with the recent advances in recombinant DNA technology, leading to increase in the range of antibodies, probes, and even systems. This review article describes ELISA and its applications for the detection of plant secondary metabolites.
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Cites
Immunoassay of endogenous plasma insulin in man
Yalow, R. S., & Berson, S. A. (1960) · Journal of Clinical Investigation · doi:10.1172/jci104130 · Cited by 3,041
Cited by
Worldwide aflatoxin contamination of agricultural products and foods: From occurrence to control
Jallow, A., Xie, H., Tang, X., Qi, Z., & Li, P. (2021) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12734 · Cited by 363
Timsina, J., Gomez-Fonseca, D., Wang, L., Do, A., Western, D., Alvarez, I., Aguilar, M., Pastor, P., Henson, R. L., Herries, E., et al. (2022) · Journal of Alzheimer’s Disease · doi:10.3233/jad-220399 · Cited by 68
Metadata
- Authors
- Sakamoto, S., Putalun, W., Vimolmangkang, S., Phoolcharoen, W., Shoyama, Y., Tanaka, H., & Morimoto, S.
- Venue
- Journal of Natural Medicines
- Published
- 2017-11-21
- Volume
- 72
- Issue
- 1
- Pages
- 32-42
- ISSN
- 1340-3443 (ISSN-L)
- 1861-0293
- OpenAlex
- W2768771467
- Cited by
- 602
- References
- 1
- FWCI
- 30.36
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Sakamoto, S., Putalun, W., Vimolmangkang, S., Phoolcharoen, W., Shoyama, Y., Tanaka, H., & Morimoto, S. (2018). Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites. Journal of Natural Medicines. https://doi.org/10.1007/s11418-017-1144-z
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