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Cited by 7Anesthesia and Sedative AgentsAnesthesia and Neurotoxicity ResearchBiomedical and Chemical Research

Anesthetic action of volatile anesthetics by using Paramecium as a model

Zhou, Miaomiao, Xia, Huimin, Xu, Younian, Xin, Naixing, Liu, Jiao, Zhang, Shihai·2012·Journal of Huazhong University of Science and Technology [Medical Sciences]
DOI 10.1007/s11596-012-0071-1
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Abstract

Although empirically well understood in their clinical administration, volatile anesthetics are not yet well comprehended in their mechanism studies. A major conundrum emerging from these studies is that there is no validated model to assess the presumed candidate sites of the anesthetics. We undertook this study to test the hypothesis that the single-celled Paramecium could be anesthetized and served as a model organism in the study of anesthetics. We assessed the motion of Paramecium cells with Expert Vision system and the chemoresponse of Paramecium cells with T-maze assays in the presence of four different volatile anesthetics, including isoflurane, sevoflurane, enflurane and ether. Each of those volatiles was dissolved in buffers to give drug concentrations equal to 0.8, 1.0, and 1.2 EC50, respectively, in clinical practice. We could see that after application of volatile anesthetics, the swimming of the Paramecium cells was accelerated and then suppressed, or even stopped eventually, and the index of the chemoresponse of the Paramecium cells (denoted as I ( che )) was decreased. All of the above impacts were found in a concentration-dependent fashion. The biphasic effects of the clinical concentrations of volatile anesthetics on Paramecium simulated the situation of high species in anesthesia, and the inhibition of the chemoresponse also indicated anesthetized. In conclusion, the findings in our studies suggested that the single-celled Paramecium could be anesthetized with clinical concentrations of volatile anesthetics and therefore be utilized as a model organism to study the mechanisms of volatile anesthetics.

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Crossref · 10.1007/s11596-012-0071-1

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  • The paramecium undergoes dose-dependent impairment of chemoresponsiveness and motility due to anesthetics.

    “the paramecium, which undergoes dose-dependent impairment of both chemoresponsiveness and motility due to anesthetics [154].”

    From The Biology of General Anesthesia from Paramecium to Primate (2019) · cites this paper as [154]

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Authors
Zhou, M., Xia, H., Xu, Y., Xin, N., Liu, J., & Zhang, S.
Affiliations
Wuhan Union Hospital (CN)
Corresponding author
  • Zhou, Miaomiao
  • Zhang, Shihai
ORCID
Venue
Journal of Huazhong University of Science and Technology [Medical Sciences]
Publisher
Springer Science and Business Media LLC
Published
2012-06
Volume
32
Issue
3
Pages
410-414
ISSN
  • 1672-0733 (ISSN-L)
  • 1993-1352
DOI
10.1007/s11596-012-0071-1
OpenAlex
W2471702808
Cited by
7
References
30
Citations by year
2023: 1 · 2022: 1 · 2021: 1 · 2019: 2 · 2018: 1 · 2017: 1
FWCI
0.00
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Journal of Huazhong University of Science and Technology [Medical Sciences] (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Anesthesiology and Pain Medicine › Anesthesia and Sedative Agents
  • Life Sciences › Neuroscience › Developmental Neuroscience › Anesthesia and Neurotoxicity Research
  • Health Sciences › Medicine › Surgery › Biomedical and Chemical Research
Keywords
volatile anesthetics, Paramecium, model organisms, isoflurane, sevoflurane, enflurane, ethers, cell motility, biphasic dose-response, concentration-dependent effects
MeSH terms
Biological Assay, Cell Movement, Chemotaxis, Dose-Response Relationship, Drug, Drug Evaluation, Preclinical, Paramecium tetraurelia, Anesthetics, Inhalation, Volatile Organic Compounds

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