Sites of alcohol and volatile anaesthetic action on GABAA and glycine receptors
Abstract
Volatile anaesthetics have historically been considered to act in a nonspecific manner on the central nervous system. More recent studies, however, have revealed that the receptors for inhibitory neurotransmitters such as gamma-aminobutyric acid (GABA) and glycine are sensitive to clinically relevant concentrations of inhaled anaesthetics. The function of GABA(A) and glycine receptors is enhanced by a number of anaesthetics and alcohols, whereas activity of the related GABA rho1 receptor is reduced. We have used this difference in pharmacology to investigate the molecular basis for modulation of these receptors by anaesthetics and alcohols. By using chimaeric receptor constructs, we have identified a region of 45 amino-acid residues that is both necessary and sufficient for the enhancement of receptor function. Within this region, two specific amino-acid residues in transmembrane domains 2 and 3 are critical for allosteric modulation of both GABA(A) and glycine receptors by alcohols and two volatile anaesthetics. These observations support the idea that anaesthetics exert a specific effect on these ion-channel proteins, and allow for the future testing of specific hypotheses of the action of anaesthetics.
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Anesthetic actions on pLGICs are not limited to GABAA receptors and are not unique to mammals.
“Anesthetic actions on pLGICs are not limited solely to GABAA receptors [41–44] and the modulatory actions of anesthetics on pLGIC family members are not unique to mammalian systems [45, 46].”
From The Biology of General Anesthesia from Paramecium to Primate (2019) · cites this paper as [42]
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- Authors
- Mihic, S. J., Ye, Q., Wick, M. J., Koltchine, V. V., Krasowski, M. D., Finn, S. E., Mascia, M. P., Valenzuela, C. F., Hanson, K. K., Greenblatt, E. P., Harris, R. A., & Harrison, N. L.
- Affiliations
- Atrium Health Wake Forest Baptist (US)
- University of Chicago (US)
- Denver VA Medical Center (US)
- University of Colorado Denver (US)
- University of Pennsylvania (US)
- Corresponding author
- Harrison, Neil L.
- ORCID
- Venue
- Nature
- Published
- 1997-09
- Volume
- 389
- Issue
- 6649
- Pages
- 385-389
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1668093331
- Cited by
- 1,219
- References
- 27
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 13 · 2023: 7 · 2022: 11 · 2021: 18 · 2020: 23 · 2019: 21 · 2018: 20 · 2017: 33 · 2016: 34 · 2015: 30 · 2014: 45 · 2013: 50 · 2012: 52
- FWCI
- 25.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Anesthesiology and Pain Medicine › Anesthesia and Sedative Agents
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Keywords
- volatile anesthetics, alcohol, GABAA receptor, glycine receptor, allosteric modulation, transmembrane domain, inhibitory neurotransmitter receptors, ion channels
- MeSH terms
- Alanine, Ethanol, Amino Acid Sequence, Animals, Binding Sites, Cell Line, Electrophysiology, Enflurane, Glycine, Humans, Molecular Sequence Data, Receptors, GABA-A, Recombinant Fusion Proteins, Serine, Tryptophan, Xenopus, Propofol, Mutagenesis, Sequence Homology, Amino Acid, Receptors, Glycine, Anesthetics, Inhalation, Anesthetics, Intravenous
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