Resveratrol Induces Pro-Oxidant Effects and Time-Dependent Resistance to Cytotoxicity in Activated Hepatic Stellate Cells
Abstract
Resveratrol (RSV) is known for its antioxidant properties; however, this compound has been proposed to have cytotoxic and pro-oxidant effects depending on its concentration and time of exposure. We previously reported the cell cycle arrest effect of low doses of RSV in GRX cells, an activated hepatic stellate cell model. Here, we evaluated the effects of RSV treatment (0.1-50 μM) for 24 and 120 h on GRX viability and oxidative status. Only treatment with 50 μM of RSV reduced the amount of live cells. However, even low doses of RSV induced an increased reactive species production at both treatment times. While being diminished within 24 h, RSV induced an increase in the SOD activity in 120 h. The cellular damage was substantially increased at 24 h in the 50 μM RSV-treated group, as indicated by the high lipoperoxidation, which may be related to the significant cell death and low proliferation. Paradoxically, this cellular damage and lipoperoxidation were considerably reduced in this group after 120 h of treatment while the surviving cells proliferated. In conclusion, RSV induced a dose-dependent pro-oxidant effect in GRX cells. The highest RSV dose induced oxidative-related damage, drastically reducing cell viability; but this cytotoxicity seems to be attenuated during 120 h of treatment.
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Some studies have highlighted RSV's ability to also act as a pro-oxidant molecule, and this dual role depends upon cell type, used dosage, and exposure time.
“Although RSV’s antioxidant properties have been widely demonstrated, some studies [349,350] have highlighted its ability to also act as a pro-oxidant molecule. This dual role depends upon cell type, used dosage, and exposure time [336].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [350]
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- Authors
- Martins, L. A. M., Coelho, B. P., Behr, G., Pettenuzzo, L. F., Souza, I. C. C., Moreira, J. C. F., Borojevic, R., Gottfried, C., & Guma, F. C. R.
- Affiliations
- Universidade Federal do Rio Grande do Sul (BR)
- ORCID
- Venue
- Cell Biochemistry and Biophysics
- Published
- 2013-06-21
- Volume
- 68
- Issue
- 2
- Pages
- 247-257
- ISSN
- 1085-9195 (ISSN-L)
- 1559-0283
- OpenAlex
- W1987927672
- Cited by
- 82
- References
- 72
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 8 · 2023: 7 · 2022: 12 · 2021: 8 · 2020: 13 · 2019: 6 · 2018: 2 · 2017: 3 · 2016: 4 · 2015: 4 · 2014: 4
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell Biochemistry and Biophysics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- resveratrol, pro-oxidant activity, hepatic stellate cells, cytotoxicity, oxidative stress, reactive oxygen species, superoxide dismutase, lipid peroxidation, cell viability, cell proliferation, cell cycle arrest, dose-dependent effects, time-dependent resistance
- MeSH terms
- Resveratrol, Animals, Anti-Inflammatory Agents, Non-Steroidal, Catalase, Cell Line, Cell Survival, Stilbenes, Superoxide Dismutase, Time Factors, Lipid Peroxidation, Reactive Oxygen Species, Oxidative Stress, Mice, Hepatic Stellate Cells
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