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Cited by 35Extracellular vesicles in diseaseMesenchymal stem cell researchLiver physiology and pathology

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Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic Liver Injury

Gupta, Suchi, Pinky, Vishal, Sharma, Harshita, Soni, Naina, Rao, E Pranshu, Dalela, Manu, Yadav, Alka, Nautiyal, Nidhi, Kumar, Anupam, Nayak, Baibaswata, Banerjee, Arup, Dinda, Amit Kumar, Mohanty, Sujata·2022·Stem Cell Reviews and Reports
DOI 10.1007/s12015-021-10313-9
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Abstract

Mesenchymal Stem Cells (MSCs) derived Extracellular Vesicles (EVs) have emerged as an effective candidate for amelioration of liver fibrosis. However, the effect and the mechanisms of MSC-EVs in liver repair remains elusive. In this study, we have evaluated the differential regenerative efficacy of EVs derived from two different human tissue-specific MSCs (Adipose tissue; AD-MSC and Wharton's Jelly; WJ-MSC), in a murine model of chronic liver fibrosis. Mouse model of chronic liver injury was induced by carbon tetrachloride (CCl 4) injection, followed by administration of EVs via the tail vein. Both quantitative and qualitative assessment was done to evaluate the hepatic regenerative potential of tissue specific MSC-extracellular vesicles. EVs, regardless of their MSC source, were found to be effective in alleviating chronic liver fibrosis, as demonstrated by macroscopic alterations in the liver. According to the findings of the comprehensive study, there were subtle variations in the tissue specific MSCs-EVs mediated approaches. A greater anti-fibrotic impact was demonstrated by AD-MSC derived EVs through extracellular matrix alteration and hepatocyte proliferation. WJ-MSC EVs, on the other hand, have an anti-inflammatory effect, as evidenced by alterations in the expression of pro- and anti-inflammatory cytokines. Furthermore, cargo profiling of these EVs revealed differences in the miRNA and protein expression, as well as the pathways that they were associated. Comparative overview of regression of fibrosis using tissue specific MSC derived EVs (credits BioRender.com ).

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Metadata

Authors
Gupta, S., Pinky, Vishal, Sharma, H., Soni, N., Rao, E. P., Dalela, M., Yadav, A., Nautiyal, N., Kumar, A., Nayak, B., Banerjee, A., Dinda, A. K., & Mohanty, S.
Affiliations
  • All India Institute of Medical Sciences (IN)
  • Regional Centre for Biotechnology (IN)
  • Institute of Liver and Biliary Sciences (IN)
Corresponding author
Mohanty, Sujata
ORCID
Venue
Stem Cell Reviews and Reports
Publisher
Springer Science and Business Media LLC
Published
2021-12-02
Volume
18
Issue
3
Pages
1097-1112
ISSN
  • 1550-8943 (ISSN-L)
  • 2629-3269
  • 2629-3277
DOI
10.1007/s12015-021-10313-9
OpenAlex
W3216878350
Cited by
35
References
2
Citations by year
2026: 1 · 2025: 7 · 2024: 15 · 2023: 9 · 2022: 3
FWCI
2.12
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Stem Cell Reviews and Reports (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords
mesenchymal stem cell-derived extracellular vesicles, liver fibrosis, chronic liver injury, carbon tetrachloride, adipose-derived stem cells, Wharton's jelly mesenchymal stem cells, liver regeneration, extracellular matrix, hepatocyte proliferation, anti-inflammatory activity, cytokine expression, EV cargo
MeSH terms
Extracellular Vesicles, Animals, Anti-Inflammatory Agents, Fibrosis, Liver Cirrhosis, Mice, Mesenchymal Stem Cells

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CoChat. (2026). Citation check: Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic Liver Injury. https://app.cochat.ai/papers/10.1007/s12015-021-10313-9
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Gupta, S., Pinky, Vishal, Sharma, H., Soni, N., Rao, E. P., Dalela, M., Yadav, A., Nautiyal, N., Kumar, A., Nayak, B., Banerjee, A., Dinda, A. K., & Mohanty, S. (2022). Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic Liver Injury. Stem Cell Reviews and Reports. https://doi.org/10.1007/s12015-021-10313-9