Exosome: A Review of Its Classification, Isolation Techniques, Storage, Diagnostic and Targeted Therapy Applications
Open accessAbstract
Exosomes are nano-sized small extracellular vesicles secreted by cells, carrying nucleic acids, proteins, lipids and other bioactive substances to play a role in the body's physiological and pathological processes. Compared to synthetic carriers such as liposomes and nanoparticles, the endogeneity and heterogeneity of exosomes give them extensive and unique advantages in the field of disease diagnosis and treatment. However, the storage stability, low yield, low purity, and weak targeting of exosomes limit its clinical application. For this reason, further exploration is needed to optimize the above problems and facilitate future functional studies of exosomes. In this paper, the origin, classification, preparation and characterization, storage stability and applications of exosome delivery system are summarized and discussed by searching a large number of literatures.
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ExoDx Lung (ALK) could detect EML4-ALK mutations in patients with non-small cell lung cancer in real time.
“In 2016, the world’s first cancer diagnostic product based on exosome miRNAs body fluid biopsy, ExoDx Lung (ALK), was launched, which could detect EML4-ALK mutations in patients with non-small cell lung cancer in real time [113].”
From The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System (2022) · cites this paper as [113]
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- Authors
- Zhang, Y., Bi, J., Huang, J., Tang, Y., Du, S., & Li, P.
- Affiliations
- Beijing University of Chinese Medicine (CN)
- Corresponding author
- Zhang, Yi
- Huang, Jiayi
- Tang, Yanan
- Du, Shouying
- Li, Pengyue
- Venue
- International Journal of Nanomedicine
- Publisher
- Informa UK Limited
- Published
- 2020-09
- Volume
- Volume 15
- Pages
- 6917-6934
- ISSN
- 1176-9114 (ISSN-L)
- 1178-2013
- PMC
- PMC7519827
- OpenAlex
- W3087179115
- Cited by
- 1,323
- References
- 140
- Citations by year
- 2026: 163 · 2025: 316 · 2024: 294 · 2023: 262 · 2022: 200 · 2021: 85 · 2020: 2 · 2016: 1
- FWCI
- 13.13
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Nanomedicine (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
- Funders
- National Natural Science Foundation of China (81803738)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- exosomes, extracellular vesicles, exosome isolation, exosome-based diagnosis, exosome characterization
- MeSH terms
- Food, Freeze Drying, Humans, Molecular Biology, Nucleic Acids, Proteins, Cryopreservation, Drug Delivery Systems, Exosomes, Molecular Targeted Therapy
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