Cited by 1,323Extracellular vesicles in diseaseNanoparticle-Based Drug DeliveryCellular transport and secretion

Exosome: A Review of Its Classification, Isolation Techniques, Storage, Diagnostic and Targeted Therapy Applications

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Zhang, Yi, Bi, Jiayao, Huang, Jiayi, Tang, Yanan, Du, Shouying, Li, Pengyue·2020·International Journal of Nanomedicine

Abstract

Exosomes are nano-sized small extracellular vesicles secreted by cells, carrying nucleic acids, proteins, lipids and other bioactive substances to play a role in the body's physiological and pathological processes. Compared to synthetic carriers such as liposomes and nanoparticles, the endogeneity and heterogeneity of exosomes give them extensive and unique advantages in the field of disease diagnosis and treatment. However, the storage stability, low yield, low purity, and weak targeting of exosomes limit its clinical application. For this reason, further exploration is needed to optimize the above problems and facilitate future functional studies of exosomes. In this paper, the origin, classification, preparation and characterization, storage stability and applications of exosome delivery system are summarized and discussed by searching a large number of literatures.

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  1. [111]Zhang XY, Zheng H, Wang YQ, et al. Protective effects of catalpol exosomes on damaged SH-SY5Y cells induced by low serum medium. Global Tradit Chin Med 2017;10(2):155–158. doi: 10.3969/j.issn.1674-1749.2017.02.006 · DOI

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Authors
Zhang, Y., Bi, J., Huang, J., Tang, Y., Du, S., & Li, P.
Affiliations
Beijing University of Chinese Medicine (CN)
Corresponding author
  • Zhang, Yi
  • Huang, Jiayi
  • Tang, Yanan
  • Du, Shouying
  • Li, Pengyue
Venue
International Journal of Nanomedicine
Published
2020-09
Volume
Volume 15
Pages
6917-6934
ISSN
  • 1176-9114 (ISSN-L)
  • 1178-2013
OpenAlex
W3087179115
Cited by
1,323
References
140
Citations by year
2026: 163 · 2025: 316 · 2024: 294 · 2023: 262 · 2022: 200 · 2021: 85 · 2020: 2 · 2016: 1
FWCI
13.13
Type
journal article
Language
en
Open access
Locations
  • International Journal of Nanomedicine (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
Funders
National Natural Science Foundation of China (81803738)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
exosomes, extracellular vesicles, exosome isolation, exosome-based diagnosis, exosome characterization
MeSH terms
Food, Freeze Drying, Humans, Molecular Biology, Nucleic Acids, Proteins, Cryopreservation, Drug Delivery Systems, Exosomes, Molecular Targeted Therapy

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