Cited by 80Nerve injury and regenerationAutophagy in Disease and TherapyHereditary Neurological Disorders

Autophagy Promotes Peripheral Nerve Regeneration and Motor Recovery Following Sciatic Nerve Crush Injury in Rats

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Huang, Hai-cheng, Chen, Li, Zhang, Hai-xing, Li, Sheng-fa, Liu, Pei, Zhao, Tian-yun, Li, Chuan-xiang·2016·Journal of Molecular Neuroscience

Abstract

Autophagy maintains cellular homeostasis by stimulating the lysosomal degradation of cytoplasmic structures, including damaged organelles and dysfunctional proteins. The role of autophagy in the renewal and regeneration of injured peripheral nerves remains poorly understood. The current study investigated the role of autophagy in peripheral nerve regeneration and motor function recovery following sciatic nerve crush injury in rats by stimulating or suppressing autophagy and detecting the presence of autophagosomes and LC3-II expression by electron microscopy and Western blotting, respectively. Neurobehavioral function was tested by CatWalk gait analysis 1, 2, 3, and 6 weeks after injury, and the expression of neurofilament (NF)-200 and myelin basic protein (MBP) at the injury site was examined by immunocytochemistry. Apoptosis at the lesion site was determined by the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Treatment of injured rats with the autophagy inducer rapamycin increased the number of autophagosomes and LC3-II expression while reducing the number of apoptotic cells at the lesion; this was associated with an upregulation of MBP and NF-200 expression and increased motor function recovery as compared to sham-operated rats and those that were subjected to crush injury but untreated. The opposite effects were observed in rats treated with the autophagy inhibitor 3-methyladenine. These data indicate that the modulation of autophagy in peripheral nerve injury could be an effective pharmacological approach to promote nerve regeneration and reestablish motor function.

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  • Stimulating Schwann cell autophagy with rapamycin promotes myelination and improves peripheral nerve regeneration.

    “Conversely, stimulating Schwann cell autophagy with rapamycin promotes myelination and improves peripheral nerve regeneration [465,466].”

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Authors
Huang, H.-C., Chen, L., Zhang, H.-X., Li, S.-F., Liu, P., Zhao, T.-Y., & Li, C.-X.
Affiliations
Third Affiliated Hospital of Southern Medical University (CN)
Corresponding author
Li, Chuan-xiang
Venue
Journal of Molecular Neuroscience
Published
2016-01-07
Volume
58
Issue
4
Pages
416-423
ISSN
  • 0895-8696 (ISSN-L)
  • 1559-1166
OpenAlex
W2225840409
Cited by
80
References
52
Citations by year
2026: 4 · 2025: 9 · 2024: 14 · 2023: 9 · 2022: 8 · 2021: 10 · 2020: 8 · 2019: 7 · 2018: 7 · 2017: 3 · 2016: 1
FWCI
3.37
Type
journal article
Language
en
Open access
Locations
  • Journal of Molecular Neuroscience (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Southern Medical University
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Hereditary Neurological Disorders
Keywords
autophagy, peripheral nerve regeneration, sciatic nerve crush injury, motor recovery, rapamycin, 3-methyladenine, LC3-II, autophagosomes, apoptosis, myelin basic protein, neurofilaments, gait analysis, peripheral nerve injury
MeSH terms
Animals, Autophagy, Myelin Basic Protein, Female, Microtubule-Associated Proteins, Movement, Nerve Regeneration, Sciatic Nerve, Neurofilament Proteins, Rats, Sprague-Dawley, Apoptosis, Rats, Peripheral Nerve Injuries

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