Ghrelin-induced enhancement of vasopressin and oxytocin secretion in rat neurohypophyseal cell cultures
Abstract
The effects of ghrelin on vasopressin and oxytocin secretion were studied in 13-14-day cell cultures of isolated rat neurohypophyseal tissue. The vasopressin and oxytocin contents of the supernatant were determined by radioimmunoassay after a 1- or 2-h incubation. Significantly increased levels of vasopressin and oxytocin production were detected in the cell culture media following ghrelin administration, depending on the ghrelin doses. The oxytocin level proved to be more elevated than that of vasopressin. The increase of vasopressin and oxytocin secretion could be totally blocked by previous administration of the ghrelin receptor antagonist ([D-Lys 3]-growth hormone-releasing peptide-6). Application of the ghrelin receptor antagonist after ghrelin administration proved ineffective. The results indicate that vasopressin and oxytocin release is influenced directly by the ghrelin system, and the effects of ghrelin on vasopressin and oxytocin secretion from the neurohypophyseal tissue in rats can occur at the level of the posterior pituitary. Our observations lend support to the view that neurohypophysis contains ghrelin receptors.
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In vitro exposure of neurohypophyseal cells to ghrelin resulted in enhanced oxytocin secretion from the cells.
“In contrast, in vitro exposure of neurohypophyseal cells to ghrelin resulted in enhanced oxytocin secretion from the cells (Galfi et al., 2016).”
From The oxytocin receptor represents a key hub in the GPCR heteroreceptor network: Potential relevance for brain and behavior (2022) · cites this paper as Galfi et al., 2016
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A ghrelin receptor and oxytocin receptor heterocomplex impairs oxytocin mediated signalling
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- Authors
- Gálfi, M., Radács, M., Molnár, Z., Budai, I., Tóth, G., Pósa, A., Kupai, K., Szalai, Z., Szabó, R., Molnár, H. A., Gardi, J., László, F. A., & Varga, C.
- Affiliations
- University of Szeged (HU)
- Corresponding author
- László, Ferenc A.
- Venue
- Journal of Molecular Neuroscience
- Published
- 2016-10-17
- Volume
- 60
- Issue
- 4
- Pages
- 525-530
- ISSN
- 0895-8696 (ISSN-L)
- 1559-1166
- OpenAlex
- W2535343878
- Cited by
- 13
- References
- 57
- Citations by year
- 2025: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 3
- FWCI
- 3.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- SZTE Publicatio Repozitórium (University of Szeged) (acceptedVersion)
- Funders
- Emberi Eroforrások Minisztériuma
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- ghrelin, vasopressin, oxytocin, neurohypophysis, growth hormone secretagogue receptor, [D-Lys3]-GHRP-6, hormone secretion
- MeSH terms
- Animals, Cell Line, Cells, Cultured, Exocytosis, Hormones, Male, Oligopeptides, Oxytocin, Pituitary Gland, Vasopressins, Rats, Wistar, Rats, Ghrelin, Receptors, Ghrelin, Neuroendocrine Cells
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