The Thlaspi caerulescens NRAMP homologue TcNRAMP3 is capable of divalent cation transport
Abstract
The NRAMP gene family encodes integral membrane protein and mediates the transport of Fe, however, its function in transport of toxic metal ions is not very clear in plants. TcNRAMP3 was isolated from Thlaspi caerulescens, and encoded a metal transporter member of the NRAMP family. TcNRAMP3 was predominantly expressed in roots of T. caerulescens by semi-quantitative RT-PCR. The expression of TcNRAMP3 was induced by iron starvation and by the heavy metals Cd and Ni in roots. TcNRAMP3 was able to rescue growth of an iron uptake fet3fet4 mutant yeast strain, suggesting a possible role in iron transport. Expression of TcNRAMP3 in yeast increased Cd sensitivity and Cd content, while it enhanced the Ni resistance and reduced Ni accumulation, indicating that TcNRAMP3 could accumulate Cd and exclude Ni in yeast. Furthermore, overexpression of TcNRAMP3 in tobacco resulted in slight Cd sensitivity of root growth and did not influence Ni resistance. These results suggested that TcNRAMP3 played a role in metal cation homeostasis in plant.
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Cd hyperaccumulation and tolerance are modulated by overexpression of NRAMPs transporters in N. caerulescens.
“Cd hyperaccumulation and tolerance are modulated by overexpression of NRAMPs transporters in N. caerulescens (Oomen et al., 2009; Wei et al., 2009; Takahashi et al., 2011).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Wei et al., 2009
Cites
Church, G. M., & Gilbert, W. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.7.1991 · Cited by 8,213 · see citing works
Baker, A. J. M., & Brooks, R. R. (1989) · Cited by 2,213 · see citing works
A novel iron-regulated metal transporter from plants identified by functional expression in yeast.
Eide, D. J., Broderius, M. A., Fett, J. P., & Guerinot, M. L. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.11.5624 · Cited by 1,403 · see citing works
Phylogenetic Relationships within Cation Transporter Families of Arabidopsis
Mäser, P., Thomine, S., Schroeder, J. I., Ward, J. M., Hirschi, K. D., Sze, H., Talke, I. N., Anna Amtmann, A., Maathuis, F. J. M., Sanders, D., et al. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.126.4.1646 · Cited by 1,257 · see citing works
Microcytic anaemia mice have a mutation in Nramp2, a candidate iron transporter gene
Fleming, M. D., Trenor, C. C., Su, M. A., Foernzler, D., Beier, D. R., Dietrich, W. F., & Andrews, N. C. (1997) · Nature Genetics · doi:10.1038/ng0897-383 · Cited by 1,159 · see citing works
Natural resistance to infection with intracellular parasites: isolation of a candidate for Bcg
Vidal, S. M., Malo, D., Vogan, K., Skamene, E., & Gros, P. (1993) · Cell · doi:10.1016/0092-8674(93)90135-d · Cited by 1,104 · see citing works
Thomine, S., Wang, R., Ward, J. M., Crawford, N. M., & Schroeder, J. I. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.9.4991 · Cited by 905 · see citing works
Emerging mechanisms for heavy metal transport in plants
Williams, L. E., Pittman, J. K., & Hall, J. L. (2000) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(00)00133-4 · Cited by 631 · see citing works
Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron
Lanquar, V., Lelièvre, F., Bolte, S., Hamès, C., Alcon, C., Neumann, D., Vansuyt, G., Curie, C., Schröder, A., Krämer, U., et al. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600864 · Cited by 631 · see citing works
Involvement of NRAMP1 from Arabidopsis thaliana in iron transport
Curie, C., Alonso, J. M., Jean, M. L., Ecker, J. R., & Briat, J. (2000) · Biochemical Journal · doi:10.1042/bj3470749 · Cited by 552 · see citing works
AtNRAMP3, a multispecific vacuolar metal transporter involved in plant responses to iron deficiency
Thomine, S., Lelièvre, F., Debarbieux, E., Schroeder, J. I., & Barbier‐Brygoo, H. (2003) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01760.x · Cited by 480 · see citing works
Divalent-metal transport by NRAMP proteins at the interface of host–pathogen interactions
Forbes, J. R., & Gros, P. (2001) · Trends in Microbiology · doi:10.1016/s0966-842x(01)02098-4 · Cited by 424 · see citing works
Supek, F., Supekova, L., Nelson, H., & Nelson, N. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.10.5105 · Cited by 325 · see citing works
The FET4 gene encodes the low affinity Fe(II) transport protein of Saccharomyces cerevisiae
Dix, D., Bridgham, J. T., Broderius, M. A., Byersdorfer, C. A., & Eide, D. J. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47163-3 · Cited by 320 · see citing works
Metal ion transporters and homeostasis
Nelson, N. (1999) · The EMBO Journal · doi:10.1093/emboj/18.16.4361 · Cited by 316 · see citing works
Ouziad, F., Hildebrandt, U., Schmelzer, E., & Bothe, H. (2005) · Journal of Plant Physiology · doi:10.1016/j.jplph.2004.09.014 · Cited by 231 · see citing works
Bereczky, Z., Wang, H., Schubert, V., Ganal, M. W., & Bauer, P. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m301365200 · Cited by 196 · see citing works
Kaiser, B. N., Moreau, S., Castelli, J. M., Thomson, R., Lambert, A., Bogliolo, S., Puppo, A., & Day, D. A. (2003) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01802.x · Cited by 181 · see citing works
Mizuno, T., Usui, K., Horie, K., Nosaka, S., Mizuno, N., & Obata, H. (2005) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2005.07.006 · Cited by 164 · see citing works
Macrophage NRAMP1 and its role in resistance to microbial infections
Govoni, G. R., & Gros, P. (1998) · Inflammation Research · doi:10.1007/s000110050330 · Cited by 163 · see citing works
Belouchi, A., Kwan, T., & Gros, P. (1997) · Plant Molecular Biology · doi:10.1023/a:1005723304911 · Cited by 157 · see citing works
Regulation and function of AtNRAMP4 metal transporter protein
Lanquar, V., Lelièvre, F., Barbier‐Brygoo, H., & Thomine, S. (2004) · Soil Science & Plant Nutrition · doi:10.1080/00380768.2004.10408587 · Cited by 65 · see citing works
Zhou, X., & Yang, Y. (2004) · Physiological and Molecular Plant Pathology · doi:10.1016/j.pmpp.2005.02.007 · Cited by 25 · see citing works
Cellier, M. F., & Gros, P. (2004) · Medical Entomology and Zoology · Cited by 19 · see citing works
Ebato, M., Matsui, N., Nomura, M., et al. (2004) · Soil Science & Plant Nutrition · doi:10.1080/00380768.2004.10408503 · Cited by 10 · see citing works
PCR-enriched cDNA pool method for cloning of gene homologues
Lang, M., Zhang, Y., Guan, Z., et al. (2005) · Plant Molecular Biology Reporter · doi:10.1007/bf02772752 · Cited by 4 · see citing works
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The OsNRAMP1 iron transporter is involved in Cd accumulation in rice
Takahashi, R., Ishimaru, Y., Senoura, T., Shimo, H., Ishikawa, S., Arao, T., Nakanishi, H., & Nishizawa, N. K. (2011) · Journal of Experimental Botany · doi:10.1093/jxb/err136 · Cited by 591 · see citing works
Oomen, R. J. F. J., Wu, J., Lelièvre, F., Blanchet, S., Richaud, P., Barbier‐Brygoo, H., Aarts, M. G. M., & Thomine, S. (2009) · New Phytologist · doi:10.1111/j.1469-8137.2008.02694.x · Cited by 270 · see citing works
Bhat, B. A., Rather, M. A., Bilal, T., Nazir, R., Qadir, R. U., & Mir, R. A. (2025) · Frontiers in Plant Science · doi:10.3389/fpls.2025.1631378 · Cited by 68 · see citing works
- Authors
- Wei, W., Chai, T., Zhang, Y., Han, L., Xu, J., & Guan, Z.
- Affiliations
- University of Chinese Academy of Sciences (CN)
- China University of Mining and Technology - Beijing (CN)
- Corresponding author
- Chai, Tuanyao
- Venue
- Molecular Biotechnology
- Published
- 2008-07-29
- Volume
- 41
- Issue
- 1
- Pages
- 15-21
- ISSN
- 1073-6085 (ISSN-L)
- 1559-0305
- OpenAlex
- W2086260705
- Cited by
- 94
- References
- 26
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 4 · 2023: 5 · 2022: 7 · 2021: 2 · 2020: 8 · 2019: 1 · 2018: 9 · 2017: 7 · 2016: 8 · 2015: 9 · 2014: 2 · 2013: 4 · 2012: 10
- FWCI
- 4.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
- Physical Sciences › Chemistry › Analytical Chemistry › Heavy Metals in Plants
- Keywords
- Thlaspi caerulescens, divalent cation transport, cadmium accumulation, nickel resistance, heavy metal homeostasis, iron starvation, iron absorption, yeast complementation, cadmium sensitivity
- MeSH terms
- Iron Deficiencies, Natural Resistance-associated Macrophage Protein 1, Cadmium, Cations, Divalent, Cloning, Molecular, Iron, Nickel, Phylogeny, Plant Proteins, Saccharomyces cerevisiae, Stress, Physiological, Nicotiana, Gene Expression Regulation, Fungal, Polymerase Chain Reaction, Ion Transport, Plant Roots, Gene Dosage, Cation Transport Proteins, Plants, Genetically Modified, Thlaspi
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