The OsNRAMP1 iron transporter is involved in Cd accumulation in rice
Open accessAbstract
Cadmium (Cd) is a heavy metal toxic to humans and the accumulation of Cd in the rice grain is a major agricultural problem, particularly in Asia. The role of the iron transporter OsNRAMP1 in Cd uptake and transport in rice was investigated here. An OsNRAMP1:GFP fusion protein was localized to the plasma membrane in onion epidermal cells. The growth of yeast expressing OsNRAMP1 was impaired in the presence of Cd compared with yeast transformed with an empty vector. Moreover, the Cd content of OsNRAMP1-expressing yeast exceeded that of the vector control. The expression of OsNRAMP1 in the roots was higher in a high Cd-accumulating cultivar (Habataki) than a low Cd-accumulating cultivar (Sasanishiki) regardless of the presence of Cd, and the amino acid sequence of OsNRAMP1 showed 100% identity between Sasanishiki and Habataki. Over-expression of OsNRAMP1 in rice increased Cd accumulation in the leaves. These results suggest that OsNRAMP1 participates in cellular Cd uptake and Cd transport within plants, and the higher expression of OsNRAMP1 in the roots could lead to an increase in Cd accumulation in the shoots. Our results indicated that OsNRAMP1 is an important protein in high-level Cd accumulation in rice.
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Cd hyperaccumulation and tolerance are modulated by overexpression of NRAMPs transporters in N. caerulescens.
“Cd hyperaccumulation and tolerance are modulated by overexpression of NRAMPs transporters in N. caerulescens (Oomen et al., 2009; Wei et al., 2009; Takahashi et al., 2011).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Takahashi et al., 2011
The Discussion reports that overexpressing OsNRAMP1 increased Cd accumulation in rice leaves. That supports a role for an NRAMP transporter in Cd accumulation, but the paper does not show that NRAMP overexpression modulates Cd hyperaccumulation or tolerance in N. caerulescens; that species appears in the References, not in the reported experiment. (LLM, conf=0.86)
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- Authors
- Takahashi, R., Ishimaru, Y., Senoura, T., Shimo, H., Ishikawa, S., Arao, T., Nakanishi, H., & Nishizawa, N. K.
- Affiliations
- The University of Tokyo (JP)
- National Institute for Agro-Environmental Sciences (JP)
- Ishikawa Prefectural University (JP)
- Corresponding author
- Nishizawa, Naoko K.
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-06-22
- Volume
- 62
- Issue
- 14
- Pages
- 4843-4850
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- PMC
- PMC3192999
- OpenAlex
- W2169000812
- Cited by
- 591
- References
- 46
- Citations by year
- 2026: 36 · 2025: 48 · 2024: 48 · 2023: 55 · 2022: 54 · 2021: 59 · 2020: 60 · 2019: 53 · 2018: 41 · 2017: 30 · 2016: 26 · 2015: 23 · 2014: 17 · 2013: 18 · 2012: 20
- FWCI
- 18.18
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Journal of Experimental Botany (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (23248011)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Heavy Metals in Plants
- Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Keywords
- cadmium accumulation, rice, heavy metal transport, iron transporters, plasma membrane localization, yeast complementation, root expression, Sasanishiki, cultivar differences
- MeSH terms
- Amino Acid Sequence, Biological Transport, Cadmium, Cloning, Molecular, Iron, Phylogeny, Plant Proteins, Oryza, Sequence Alignment, Gene Expression Regulation, Plant, Cation Transport Proteins
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