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Cited by 15Protein Kinase Regulation and GTPase SignalingSarcoma Diagnosis and TreatmentPI3K/AKT/mTOR signaling in cancer

Ezrin silencing remodulates the expression of Phosphoinositide‐specific Phospholipase C enzymes in human osteosarcoma cell lines

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Lo Vasco, V. R., Leopizzi, M., Puggioni, C., Della Rocca, C.·2014·Journal of Cell Communication and Signaling
DOI 10.1007/s12079-014-0235-9PMC PMC4165819
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Abstract

Ezrin, a protein belonging to the Ezrin, radixin and moesin (ERM) family, was engaged in the metastatic spread of osteosarcoma. The Protein 4.1, Ezrin, radixin, moesin (FERM) domain of Ezrin binds the membrane Phosphatydil inositol (4,5) bisphosphate (PIP2), a crucial molecule belonging to the Phosphoinositide (PI) signal transduction pathway. The cytoskeleton cross‐linker function of Ezrin largely depends on membrane PIP2 levels, and thus upon the activity of related enzymes belonging to the PI‐specific phospholipase C (PI‐PLC) family. Based on the role of Ezrin in tumour progression and metastasis, we silenced the expression of Vil2 (OMIM *123900), the gene which codifies for Ezrin, in cultured human osteosarcoma 143B and Hs888 cell lines. After Ezrin silencing, the growth rate of both cell lines was significantly reduced and morphogical changes were observed. We also observed moderate variations both of selected PI‐PLC enzymes within the cell and of expression of the corresponding PLC genes. In 143B cell line the transcription of PLCB1 decreased, of PLCG2 increased and of PLCE differed in a time‐dependent manner. In Hs888, the expression of PLCB1 and of PLCD4 significantly increased, of PLCE moderately increased in a time dependent manner; the expression of PLCG2 was up‐regulated. These observations indicate that Ezrin silencing affects the transcription of selected PLC genes, suggesting that Ezrin might influence the expression regulation of PI‐PLC enzymes.

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  • siRNA targeting Ezrin in human osteosarcoma cell lines resulted in PLCγ2 upregulation.

    “siRNA targeting Ezrin, a cytoplasmic peripheral protein involved in metastatic spreading of osteosarcoma and dependent on PIP2, also resulted in PLCγ2 upregulation in human osteosarcoma cell lines (155, 156), while another study of osteosarcoma cell lines found PLCγ2 to be a differentially regulated gene (157).”

    From The role of PLCγ2 in immunological disorders, cancer, and neurodegeneration (2021) · cites this paper as [155]

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Cited by

Sorted by citations

Authors
Lo Vasco, V. R., Leopizzi, M., Puggioni, C., & Della Rocca, C.
Affiliations
  • Sense Organs Department Policlinico Umberto I Faculty of Medicine and Dentistry Sapienza University of Rome viale del Policlinico 155 00185 Italy Rome
  • Sapienza University of Rome (IT)
  • Scienze e Biotecnologie Medico Chirurgiche Department Polo Pontino Sapienza University Latina Italy
ORCID
Venue
Journal of Cell Communication and Signaling
Publisher
Wiley
Published
2014-07-30
Volume
8
Issue
3
Pages
219-229
ISSN
  • 1873-9601 (ISSN-L)
  • 1873-961X
DOI
10.1007/s12079-014-0235-9
PMC
PMC4165819
OpenAlex
W2087941837
Cited by
15
References
3
Citations by year
2024: 1 · 2022: 2 · 2021: 2 · 2020: 2 · 2017: 1 · 2016: 4 · 2015: 3
FWCI
1.09
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Cell Communication and Signaling (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Florence Research (University of Florence) (submittedVersion)
  • IRIS UNIMORE (University of Modena and Reggio Emilia) (submittedVersion, cc-by)
  • IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Sarcoma Diagnosis and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
Keywords
ezrin, osteosarcoma, phosphatidylinositol-specific phospholipase C, PLCB1, PLCγ2, gene silencing, phosphatidylinositol 4,5-bisphosphate, FERM domain, cytoskeleton, cell growth, metastasis, transcriptional regulation, 143B cells

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