Anti-inflammatory and anti-osteoporotic potential of Lactobacillus plantarum A41 and L. fermentum SRK414 as probiotics
Abstract
This study involves an investigation on the probiotic properties of lactic acid bacteria and their potential applications in an in vitro model of lipopolysaccharide (LPS)-stimulated inflammation and dexamethasone-induced osteoporosis. Nine strains were pre-screened from 485 lactic acid bacteria based on their survival at a low pH and in a solution containing bile salts. All candidates were capable of surviving in an environment with low pH and with bile salts and could successfully colonize the intestine. Furthermore, their functional properties, such as anti-oxidation and anti-inflammation, were evaluated. Of the nine probiotic candidates, Lactobacillus plantarum A41 and L. fermentum SRK414 exhibited the highest anti-oxidative capacity. Moreover, only L. plantarum A41 and L. fermentum SRK414 could increase gut barrier function by upregulating the mRNA expression of tight junction proteins and inhibit the expression of inflammatory mediators induced by LPS-stimulated inflammation. Interestingly, these two strains were also capable of regulating several bone metabolism-related markers playing a role in bone homeostasis and osteoblast differentiation. In brief, L. plantarum A41 and L. fermentum SRK414 exhibited high probiotic potential and potentially impact immune-related bone health by modulating pro-inflammatory cytokines and bone metabolism-related markers.
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The criteria for selecting probiotic LAB strains are safety, including absence of secretion of harmful metabolites, survival in gastrointestinal conditions, and ability to inhibit pathogens.
“The criteria for selecting probiotic LAB strains are safety, including the absence of the secretion of harmful metabolites, survival in gastrointestinal conditions, and the ability to inhibit pathogens [10,11].”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [11]
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- Authors
- Lee, C. S., & Kim, S. H.
- Affiliations
- Korea University (KR)
- Corresponding author
- Kim, Sae Hun
- Venue
- Probiotics and Antimicrobial Proteins
- Published
- 2019-08-02
- Volume
- 12
- Issue
- 2
- Pages
- 623-634
- ISSN
- 1867-1306 (ISSN-L)
- 1867-1314
- OpenAlex
- W2965854142
- Cited by
- 76
- References
- 50
- Citations by year
- 2026: 7 · 2025: 7 · 2024: 12 · 2023: 16 · 2022: 15 · 2021: 12 · 2020: 6 · 2019: 1
- FWCI
- 6.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Probiotics and Antimicrobial Proteins (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (117051-03-1-HD020)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- probiotics, lactic acid bacteria, anti-inflammatory activity, anti-osteoporotic activity, lipopolysaccharide-induced inflammation, dexamethasone-induced osteoporosis, intestinal barrier, tight junction proteins, bone metabolism, osteogenic differentiation, antioxidant capacity, bile salt tolerance, low pH tolerance, intestinal colonization
- MeSH terms
- Antioxidants, Cell Differentiation, Humans, Inflammation, Osteoblasts, Osteoporosis, Biomarkers, Cytokines, HT29 Cells, Probiotics, Lactiplantibacillus plantarum, Limosilactobacillus fermentum
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