Cited by 676Probiotics and Fermented FoodsGastrointestinal motility and disordersBarrier Structure and Function Studies

Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier

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Karczewski, Jurgen, Troost, Freddy J., Konings, Irene, Dekker, Jan, Kleerebezem, Michiel, Brummer, Robert-Jan M., Wells, Jerry M.·2010·American Journal of Physiology-Gastrointestinal and Liver Physiology

Abstract

Lactobacillus plantarum, a commensal bacterium of humans, has been proposed to enhance the intestinal barrier, which is compromised in a number of intestinal disorders. To study the effect of L. plantarum strain WCFS1 on human barrier function, healthy subjects were administered L. plantarum or placebo in the duodenum for 6 h by means of a feeding catheter. The scaffold protein zonula occludens (ZO)-1 and transmembrane protein occludin were found to be significantly increased in the vicinity of the tight-junction (TJ) structures, which form the paracellular seal between cells of the epithelium. In an in vitro model of the human epithelium, L. plantarum induced translocation of ZO-1 to the TJ region; however, the effects on occludin were minor compared with those seen in vivo. L. plantarum was shown to activate Toll-like receptor 2 (TLR2) signaling, and treatment of Caco-2 monolayers with the TLR2 agonist Pam(3)-Cys-SK4(PCSK) significantly increased fluorescent staining of occludin in the TJ. Pretreatment of Caco-2 monolayers with L. plantarum or PCSK significantly attenuated the effects of phorbol ester-induced dislocation of ZO-1 and occludin and the associated increase in epithelial permeability. Our results identifying commensal bacterial stimulation of TLR2 in the gut epithelium as a regulator of epithelial integrity have important implications for understanding probiotic mechanisms and the control of intestinal homeostasis.

Integrity signals

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Crossref · 10.1152/ajpgi.00327.2009

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Authors
Karczewski, J., Troost, F. J., Konings, I., Dekker, J., Kleerebezem, M., Brummer, R.-J. M., & Wells, J. M.
Affiliations
  • TiFN (NL)
  • Maastricht University (NL)
  • Örebro University (SE)
Corresponding author
Wells, Jerry M.
Venue
American Journal of Physiology-Gastrointestinal and Liver Physiology
Published
2010-06
Volume
298
Issue
6
Pages
G851-G859
ISSN
  • 0193-1857 (ISSN-L)
  • 1522-1547
OpenAlex
W2101272036
Cited by
676
References
49
Citations by year
2026: 36 · 2025: 43 · 2024: 50 · 2023: 43 · 2022: 62 · 2021: 67 · 2020: 56 · 2019: 45 · 2018: 35 · 2017: 41 · 2016: 33 · 2015: 30 · 2014: 39 · 2013: 32 · 2012: 28
FWCI
47.11
Type
journal article
Language
en
Open access
Locations
  • American Journal of Physiology-Gastrointestinal and Liver Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • Socio-Environmental Systems Modeling (acceptedVersion)
  • Research Publications (Maastricht University) (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Keywords
Lactobacillus plantarum, tight junction proteins, intestinal barrier function, zonula occludens-1, occludin, TLR2, probiotics, commensal bacteria, epithelial permeability, phorbol ester, Caco-2 cells, intestinal homeostasis
MeSH terms
Adult, Duodenum, Epithelial Cells, Humans, Intestinal Mucosa, Membrane Proteins, Phosphoproteins, Signal Transduction, Cross-Over Studies, Microscopy, Confocal, Caco-2 Cells, Tight Junctions, Cytoprotection, Lactobacillus plantarum, Lactiplantibacillus plantarum, Toll-Like Receptor 2, Occludin, Zonula Occludens-1 Protein

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