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Probiotic Lactobacillus johnsonii BS15 Prevents Memory Dysfunction Induced by Chronic High-Fluorine Intake through Modulating Intestinal Environment and Improving Gut Development

Sun, Ning, Ni, Xueqin, Wang, Hesong, Xin, Jinge, Zhao, Ying, Pan, Kangcheng, Jing, Bo, Zeng, Dong·2020·Probiotics and Antimicrobial Proteins
DOI 10.1007/s12602-020-09644-9
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Abstract

In recent years, the influence of chronic fluorosis on the brain has been widely reported. Our study aimed to demonstrate the potential mechanism underlying the impairment of memory function by excessive fluorine intake. We also evaluated whether improvement of intestinal microflora could be a potential therapy to prevent the negative influences from the perspective of gut-brain axis. Male ICR mice were randomly divided into three groups and administered with either phosphate buffered saline (PBS) (Control and F groups) or Lactobacillus johnsonii BS15 (FP group; daily amounts of 1 × 10 9 CFU/mL), a probiotic strain, by oral gavage throughout a 98-day experimental period. Sodium fluoride (100 mg/L) was added to the drinking water of the F and FP groups. Animals were sacrificed for sampling with or without water avoidance stress (WAS) at two phases of the experiment and behavioral tests including T-maze test and passive avoidance test were also performed. Based on the results of behavioral tests, probiotic reversed the fluorine-induced memory dysfunction. In addition, L. johnsonii BS15 also increased the antioxidant capacities (serum and hippocampal tissue) and hippocampal synaptic plasticity-related mRNA expression after excessive fluoride ingestion. Moreover, the increased colonization of L. johnsonii BS15 also protected the small intestines from the damages of growth performance, visceral indexes, intestinal development, digestive, and secretory functions by changing the str…

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Metadata

Authors
Sun, N., Ni, X., Wang, H., Xin, J., Zhao, Y., Pan, K., Jing, B., & Zeng, D.
ORCID
Zeng, Dong
Venue
Probiotics and Antimicrobial Proteins
Publisher
Springer Science and Business Media LLC
Published
2020-03-12
Volume
12
Issue
4
Pages
1420-1438
ISSN
  • 1867-1306
  • 1867-1314
DOI
10.1007/s12602-020-09644-9
Cited by
31
References
5
Language
en
Licence
Funders
  • National Natural Science Foundation of China (31970503)
  • Sichuan Science and Technology Program (2018HH0103)

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