Sequential Extraction and Characterisation of Lipids, Proteins, and Chitin from Black Soldier Fly (Hermetia illucens) Larvae, Prepupae, and Pupae

A review of chitin and chitosan applications

Ravi Kumar, M. N. V. (2000) · Reactive and Functional Polymers · doi:10.1016/s1381-5148(00)00038-9 · Cited by 6,009

An infrared investigation in relation with chitin and chitosan characterization

Brugnerotto, J., Lizardi‐Mendoza, J., Goycoolea, F. M., Argüelles-Monal, W. M., Desbrières, J., & Rinaudo, M. (2001) · Polymer · doi:10.1016/s0032-3861(00)00713-8 · Cited by 1,536

Functional Characterization of Chitin and Chitosan

Aranaz, I., Mengíbar, M., Harris, R., Paños, I., Miralles, B., Acosta, N., Galed, G., & Heras, Á. (2009) · Current Chemical Biology · doi:10.2174/2212796810903020203 · Cited by 1,248

Industrial applications of crustacean by-products (chitin, chitosan, and chitooligosaccharides): A review

Hamed, I., Özoğul, F., & Regenstein, J. M. (2015) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2015.11.007 · Cited by 1,152

Nutritional composition of black soldier fly ( Hermetia illucens ) prepupae reared on different organic waste substrates

Spranghers, T., Ottoboni, M., Klootwijk, C., Ovyn, A., Deboosere, S., De Meulenaer, B., Michiels, J., Eeckhout, M., De Clercq, P., & De Smet, S. (2017) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.8081 · Cited by 970

Feed Conversion, Survival and Development, and Composition of Four Insect Species on Diets Composed of Food By-Products

Oonincx, D. G. A. B., van Broekhoven, S., van Huis, A., & van Loon, J. J. A. (2015) · PLoS ONE · doi:10.1371/journal.pone.0144601 · Cited by 951

Nitrogen-to-Protein Conversion Factors for Three Edible Insects: Tenebrio molitor , Alphitobius diaperinus , and Hermetia illucens

Janssen, R. H., Vincken, J., van den Broek, L. A. M., Fogliano, V., & Lakemond, C. M. M. (2017) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.7b00471 · Cited by 904

Review of black soldier fly (Hermetia illucens) as animal feed and human food.

Wang, Y.-S., & Shelomi, M. (2017) · Foods · doi:10.3390/foods6100091 · Cited by 812

Complete nutrient composition of commercially raised invertebrates used as food for insectivores

Finke, M. D. (2002) · Zoo Biology · doi:10.1002/zoo.10031 · Cited by 802

Production, Properties, and Some New Applications of Chitin and Its Derivatives

Synowiecki, J., & Al-Khateeb, N. A. A. Q. (2003) · Critical reviews in food science and nutrition · doi:10.1080/10408690390826473 · Cited by 755

Obesity, metabolic factors and risk of different histological types of lung cancer: A Mendelian randomization study

Carreras‐Torres, R., Johansson, M., Haycock, P., Wade, K. H., Relton, C. L., Martin, R. M., Smith, G. D., Albanes, D., Aldrich, M. C., Andrew, A. S., et al. (2017) · PLoS ONE · doi:10.1371/journal.pone.0177875 · Cited by 664

Effect of rearing substrate on growth performance, waste reduction efficiency and chemical composition of black soldier fly ( Hermetia illucens ) larvae

Meneguz, M., Schiavone, A., Gai, F., Dama, A., Lussiana, C., Renna, M., & Gasco, L. (2018) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.9127 · Cited by 532

Decomposition of biowaste macronutrients, microbes, and chemicals in black soldier fly larval treatment: A review

Gold, M., Tomberlin, J. K., Diener, S., Zurbrügg, C., & Mathys, A. (2018) · Waste Management · doi:10.1016/j.wasman.2018.10.022 · Cited by 528

Black soldier fly larvae meal can replace fish meal in diets of sea-water phase Atlantic salmon (Salmo salar)

Belghit, I., Liland, N. S., Gjesdal, P., Biancarosa, I., Menchetti, E., Li, Y., Waagbø, R., Krogdahl, Å., & Lock, E. (2018) · Aquaculture · doi:10.1016/j.aquaculture.2018.12.032 · Cited by 525

Chitin characterization by SEM, FTIR, XRD, and 13 C cross polarization/mass angle spinning NMR

Cárdenas, G. T., Cabrera‐Barjas, G., Taboada, E., & Miranda, S. P. (2004) · Journal of Applied Polymer Science · doi:10.1002/app.20647 · Cited by 520

Rearing Methods for the Black Soldier Fly (Diptera: Stratiomyidae): Table 1

Sheppard, D. C., Tomberlin, J. K., Joyce, J. A., Kiser, B. C., & Sumner, S. M. (2002) · Journal of Medical Entomology · doi:10.1603/0022-2585-39.4.695 · Cited by 479

Fly Prepupae as a Feedstuff for Rainbow Trout, Oncorhynchus mykiss

St‐Hilaire, S., Sheppard, C. D., Tomberlin, J. K., Irving, S. N., Newton, L., McGuire, M. A., Mosley, E. E., Hardy, R. W., & Sealey, W. M. (2007) · Journal of the World Aquaculture Society · doi:10.1111/j.1749-7345.2006.00073.x · Cited by 459

Review of Chitosan and Its Derivatives as Antimicrobial Agents and Their Uses as Textile Chemicals

Lim, S.-H., & Hudson, S. (2003) · Journal of macromolecular science. Part C, Reviews in macromolecular chemistry and physics/Journal of macromolecular science. Reviews in macromolecular chemistry and physics · doi:10.1081/mc-120020161 · Cited by 446

Influence of different growing substrates and processing on the nutrient composition of black soldier fly larvae destined for animal feed

Tschirner, M., & Simon, A. (2015) · Journal of Insects as Food and Feed · doi:10.3920/jiff2014.0008 · Cited by 426

Dynamic changes of nutrient composition throughout the entire life cycle of black soldier fly

Liu, X., Chen, X., Wang, H., Yang, Q., ur Rehman, K., Li, W., Cai, M., Li, Q., Mazza, L., Zhang, J., et al. (2017) · PLOS ONE · doi:10.1371/journal.pone.0182601 · Cited by 407

The nutritive value of black soldier fly larvae reared on common organic waste streams in Kenya

Shumo, M., Osuga, I. M., Khamis, F. M., Tanga, C. M., Fiaboe, K. K. M., Subramanian, S., Ekesi, S., van Huis, A., & Borgemeister, C. (2019) · Scientific Reports · doi:10.1038/s41598-019-46603-z · Cited by 405

Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin

Caligiani, A., Marseglia, A., Leni, G., Baldassarre, S., Maistrello, L., Dossena, A., & Sforza, S. (2017) · Food Research International · doi:10.1016/j.foodres.2017.12.012 · Cited by 388

Capillary Column Gas Chromatographic Method for Analysis of Encapsulated Fish Oils and Fish Oil Ethyl Esters: Collaborative Study

Joseph, J. D., & ACKMAN, R. G. (1992) · Journal of AOAC International · doi:10.1093/jaoac/75.3.488 · Cited by 379

Recovery and techno-functionality of flours and proteins from two edible insect species: Meal worm ( Tenebrio molitor ) and black soldier fly ( Hermetia illucens ) larvae

Bußler, S., Rumpold, B. A., Jander, E., Rawel, H. M., & Schlüter, O. (2016) · Heliyon · doi:10.1016/j.heliyon.2016.e00218 · Cited by 371

Extraction and Characterization of Chitin from the Beetle Holotrichia parallela Motschulsky

Liu, S., Sun, J., Yu, L., Zhang, C., Bi, J., Zhu, F., Qu, M., Jiang, C., & Yang, Q. (2012) · Molecules · doi:10.3390/molecules17044604 · Cited by 370

Selected Life-History Traits of Black Soldier Flies (Diptera: Stratiomyidae) Reared on Three Artificial Diets

Tomberlin, J. K., Sheppard, D. C., & Joyce, J. A. (2002) · Annals of the Entomological Society of America · doi:10.1603/0013-8746(2002)095[0379:slhtob]2.0.co;2 · Cited by 363

Chitin Extraction from Crustacean Shells Using Biological Methods - A Review

Arbia, W., Arbia, L., Adour, L., & Amrane, A. (2012) · Hrčak Portal of scientific journals of Croatia (University Computing Centre) · Cited by 362

Bioconversion of dairy manure by black soldier fly (Diptera: Stratiomyidae) for biodiesel and sugar production

Li, Q., Zheng, L., Qiu, N., Cai, H., Tomberlin, J. K., & Yu, Z. (2011) · Waste Management · doi:10.1016/j.wasman.2011.01.005 · Cited by 355

Aging is accompanied by a blunted muscle protein synthetic response to protein ingestion

Wall, B. T., Gorissen, S. H., Pennings, B., Koopman, R., Groen, B. B. L., Verdijk, L. B., & van Loon, L. J. C. (2015) · PLOS ONE · doi:10.1371/journal.pone.0140903 · Cited by 352

Development of black soldier fly (Diptera: Stratiomyidae) larvae fed dairy manure

Myers, H. M., Tomberlin, J. K., Lambert, B. D., & Kattes, D. (2008) · Environmental Entomology · doi:10.1093/ee/37.1.11 · Cited by 338

Influence of larval density and dietary nutrient concentration on performance, body protein, and fat contents of black soldier fly larvae ( Hermetia illucens )

Barragán–Fonseca, K. B., Dicke, M., & van Loon, J. J. A. (2018) · Entomologia Experimentalis et Applicata · doi:10.1111/eea.12716 · Cited by 284

Conversion of organic resources by black soldier fly larvae: Legislation, efficiency and environmental impact

Bosch, G., van Zanten, H. H. E., Zamprogna, A., Veenenbos, M., Meijer, N., van der Fels‐Klerx, H. J., & van Loon, J. J. A. (2019) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2019.02.270 · Cited by 268

Amino acids in animal nutrition

(2003) · CABI Publishing eBooks · doi:10.1079/9780851996547.0000 · Cited by 244

Suitability of microwave drying for mealworms (Tenebrio molitor) as alternative to freeze drying: Impact on nutritional quality and colour

Lenaerts, S., Borght, M. V. D., Callens, A., & Campenhout, L. V. (2018) · Food Chemistry · doi:10.1016/j.foodchem.2018.02.006 · Cited by 218

The high level of protein content reported in insects for food and feed is overestimated

Jonas‐Levi, A., & Martinez, J. I. (2017) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2017.06.004 · Cited by 190

Effect of blanching followed by refrigerated storage or industrial microwave drying on the microbial load of yellow mealworm larvae (Tenebrio molitor)

Vandeweyer, D., Lenaerts, S., Callens, A., & Campenhout, L. V. (2016) · Food Control · doi:10.1016/j.foodcont.2016.07.011 · Cited by 189

Extraction and characterization of chitin and chitosan with antimicrobial and antioxidant activities from cosmopolitan Orthoptera species (Insecta)

Kaya, M., Baran, T., Asan-Ozusaglam, M., Çakmak, Y. S., Tozak, K. Ö., Mol, A., Menteş, A., & Sezen, G. (2015) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-014-0391-z · Cited by 188

Physicochemical comparison of chitin and chitosan obtained from larvae and adult Colorado potato beetle (Leptinotarsa decemlineata)

Kaya, M., Baran, T., Erdoğan, S., Menteş, A., Asan-Ozusaglam, M., & Çakmak, Y. S. (2014) · Materials Science and Engineering C · doi:10.1016/j.msec.2014.09.004 · Cited by 186

Evaluation of Black Soldier Fly (Hermetia illucens) Larvae and Pre-Pupae Raised on Household Organic Waste, as Potential Ingredients for Poultry Feed

Kawasaki, K., Hashimoto, Y., Hori, A. N., Kawasaki, T., Hirayasu, H., Iwase, S., Hashizume, A., Ido, A., Miura, C., Miura, T., et al. (2019) · Animals · doi:10.3390/ani9030098 · Cited by 173

Effects of temperature and diet on black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), development

Harnden, L. M., & Tomberlin, J. K. (2016) · Forensic Science International · doi:10.1016/j.forsciint.2016.05.007 · Cited by 171

The first report of the physicochemical structure of chitin isolated from Hermetia illucens

Waśko, A. D., Bulak, P., Polak‐Berecka, M., Nowak, K., Polakowski, C., & Bieganowski, A. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.07.038 · Cited by 165

Development of Tungsten Oxide Nanoparticle Modified Carbon Fibre Cloth as Flexible pH Sensor

Jamal, M., Razeeb, K. M., Shao, H., Islam, J., Akhter, I., Furukawa, H., & Khosla, A. (2019) · Scientific Reports · doi:10.1038/s41598-019-41331-w · Cited by 146

Influence of Freeze-Drying and Oven-Drying Post Blanching on the Nutrient Composition of the Edible Insect Ruspolia differens

Fombong, F., Borght, M. V. D., & Broeck, J. V. (2017) · Insects · doi:10.3390/insects8030102 · Cited by 136

Killing method affects the browning and the quality of the protein fraction of Black Soldier Fly (Hermetia illucens) prepupae: a metabolomics and proteomic insight

Leni, G., Caligiani, A., & Sforza, S. (2018) · Food Research International · doi:10.1016/j.foodres.2018.08.021 · Cited by 114

Marine Bioactive Compounds: Sources, Characterization and Applications

(2012) · doi:10.1007/978-1-4614-1247-2 · Cited by 111

Changes in physicochemical properties of chitin at developmental stages (larvae, pupa and adult) of Vespa crabro (wasp)

Kaya, M., Sofi, K., Sargın, İ., & Mujtaba, M. (2016) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2016.03.010 · Cited by 101

Comparison of physicochemical properties of chitins isolated from an insect (Melolontha melolontha) and a crustacean species (Oniscus asellus)

Kaya, M., Baublys, V., Can, E., Šatkauskienė, I., Bitim, B., Tubelytė, V., & Baran, T. (2014) · Zoomorphology · doi:10.1007/s00435-014-0227-6 · Cited by 90

Mining marine shellfish wastes for bioactive molecules: Chitin and chitosan – Part B: Applications

Hayes, M., Carney, B., Slater, J., & Brück, W. M. (2008) · Biotechnology Journal · doi:10.1002/biot.200800027 · Cited by 74

Morphological description of the immature stages of Hermetia illucens (Linnaeus, 1758) (Diptera: Stratiomyidae)

Barros, L. M., Gutjahr, A. L. N., Ferreira-Keppler, R. L., & Martins, R. T. (2018) · Microscopy Research and Technique · doi:10.1002/jemt.23127 · Cited by 61

Insects as an Alternative Source for the Production of Fats for Cosmetics.

Verheyen, G. R., Ooms, T., Vogels, L., Vreysen, S., Bovy, A., Miert, S. V., & Meersman, F. (2018) · PubMed · Cited by 60

Evaluation of the Economic, Social, and Biological Feasibility of Bioconverting Food Wastes with the Black Soldier Fly (Hermetia illucens)

Barry, T. (2025) · doi:10.12794/metadc4639 · Cited by 50

ANALISIS COMPOSICIONAL, MICROBIOLÓGICO Y DIGESTIBILIDAD DE LA PROTEÍNA DE LA HARINA DE LARVAS DE Hermetia illuscens L (DIPTERA: STRATIOMYIIDAE) EN ANGELÓPOLIS-ANTIOQUIA, COLOMBIA

Gutiérrez, G. P. A., Ruiz, R. V., & Vélez, H. M. (2004) · Revista Facultad Nacional de Agronomía Medellín · Cited by 44

Detection of Intercatechol Cross-Links in Insect Cuticle by Solid-State Carbon-13 and Nitrogen-15 NMR

Merritt, M. E., Christensen, A. M., Kramer, K. J., Hopkins, T. L., & Schaefer, J. (1996) · Journal of the American Chemical Society · doi:10.1021/ja961621o · Cited by 43

Chitin, Chitosan and their Derivatives from Marine Rest Raw Materials: Potential Food and Pharmaceutical Applications

Hayes, M. (2011) · Springer eBooks · doi:10.1007/978-1-4614-1247-2_4 · Cited by 41

3,4-Dihydroxyphenylacetaldehyde synthase and cuticle formation in insects

Liao, C., Upadhyay, A., Liang, J., Han, Q., & Li, J. (2017) · Developmental & Comparative Immunology · doi:10.1016/j.dci.2017.11.007 · Cited by 38

Effects of substitution of fish meal with fresh and dehydrated larvae of the house fly (Musca domestica L) on productive performance and health of broilers

Đorđević, M., Radenkovic-Damnjanovic, B., Vučinić, M. M., Baltic, M., Teodorović, R., Janković, L., Vukašinović, M., & Rajkovic, M. (2008) · Acta veterinaria · doi:10.2298/avb0804357d · Cited by 35

Effect of sulfonamide pollution on the growth of manure management candidate Hermetia illucens

Gao, Q., Deng, W., Gao, Z., Li, M., Liu, W., Wang, X., & Zhu, F. (2019) · PLoS ONE · doi:10.1371/journal.pone.0216086 · Cited by 30

AN INVESTIGATION INTO INSECT PROTEIN

VALLE, F. R. D., MENA, M. H., & Bourges, H. (1982) · Journal of Food Processing and Preservation · doi:10.1111/j.1745-4549.1982.tb00645.x · Cited by 29

Rethinking organic wastes bioconversion: Evaluating the potential of the black soldier fly (Hermetia illucens (L.)) (Diptera: Stratiomyidae) (BSF)

Surendra, K. C., Tomberlin, J. K., van Huis, A., Cammack, J. A., Heckmann, L.-H. L., & Khanal, S. K. (2020) · Waste Management · doi:10.1016/j.wasman.2020.07.050 · Cited by 456

Current state of chitin purification and chitosan production from insects

Hahn, T., Tafi, E., Paul, A., Salvia, R., Falabella, P., & Zibek, S. (2020) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.6533 · Cited by 392

Nutritional Composition of Black Soldier Fly Larvae (Hermetia illucens L.) and Its Potential Uses as Alternative Protein Sources in Animal Diets: A Review

Lu, S., Taethaisong, N., Meethip, W., Surakhunthod, J., Sinpru, B., Sroichak, T., Archa, P., Thongpea, S., Paengkoum, S., Purba, R. A. P., et al. (2022) · Insects · doi:10.3390/insects13090831 · Cited by 410

Characterization of chitin and chitosan derived from Hermetia illucens, a further step in a circular economy process

Triunfo, M., Tafi, E., Guarnieri, A., Salvia, R., Scieuzo, C., Hahn, T., Zibek, S., Gagliardini, A., Panariello, L., Coltelli, M., et al. (2022) · Scientific Reports · doi:10.1038/s41598-022-10423-5 · Cited by 357

Use of black soldier fly (Hermetia illucens L.) larvae meal in aquafeeds for a sustainable aquaculture industry: A review of past and future needs

Mohan, K., Rajan, D. K., Muralisankar, T., Ganesan, A. R., Sathishkumar, P., & Revathi, N. (2022) · Aquaculture · doi:10.1016/j.aquaculture.2022.738095 · Cited by 214

Isolation and characterization of lipids, proteins and chitin from Black Soldier Fly (Hermetia illucens) larvae, prepupae and pupae

Verbinnen, B., Voorde, I. V. D., Aerts, G. M., CLAES, J. E., & Borght, M. V. D. (2018) · Lirias (KU Leuven)

Characteristics of chitin extracted from different growth phases of black soldier fly, Hermetia illucens, fed with different organic wastes

Rampure, S. M., Velayudhannair, K., & Nidhin, M. (2023) · International Journal of Tropical Insect Science · doi:10.1007/s42690-023-00997-6 · Cited by 26

Comprehensive utilization of black soldier fly (Hermetia illucens) larvae: extraction, recovery and characterization of peptide, chitin and melanin and scaling-up trial

Chen, Y., Guo, Y., He, X. Z., Tan, B., Liao, Z., Chen, A., Gu, X., Li, X., Chen, X., Chen, B., et al. (2024) · Separation and Purification Technology · doi:10.1016/j.seppur.2024.131262 · Cited by 30

Mechanical and Enzyme Assisted Fractionation Process for a Sustainable Production of Black Soldier Fly (Hermetia illucens) Ingredients

Azzollini, D., van Iwaarden, A., Lakemond, C. M. M., & Fogliano, V. (2020) · Frontiers in Sustainable Food Systems · doi:10.3389/fsufs.2020.00080 · Cited by 24

Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin

Caligiani, A., Marseglia, A., Leni, G., Baldassarre, S., Maistrello, L., Dossena, A., & Sforza, S. (2017) · Food Research International · doi:10.1016/j.foodres.2017.12.012 · Cited by 388