Cited by 19Heat shock proteins researchCardiac Ischemia and ReperfusionCell death mechanisms and regulation

Carboxy terminus of heat shock protein (HSP) 70-interacting protein (CHIP) inhibits HSP70 in the heart

Zhao, Bijun, Sun, Guocheng, Feng, Guanli, Duan, Weixun, Zhu, Xiaoling, Chen, Shaoyang, Hou, Lichao, Jin, Zhenxiao +1 more·2012·Journal of Physiology and Biochemistry

Abstract

Heat shock protein (HSP) 70 plays a critical role in protecting the heart from various stressor-induced cell injuries; the mechanism remains to be further understood. The present study aims to elucidate the effect of a probiotics-derived protein, LGG-derived protein p75 (LGP), in alleviating the ischemia/reperfusion (I/R)-induced heart injury. We treated rats with the I/R with or without preadministration with LGP. The levels of HSP70 and carboxy terminus of HSP70-interacting protein (CHIP) in the heart tissue were assessed by enzyme-linked immunosorbent assay (ELISA) and Western blotting. The effect of CHIP on suppression of HSP70 and the effect of LGP on suppression of CHIP were investigated with an I/R rat model and a cell culture model. The results showed that I/R-induced infarction in the heart could be alleviated by pretreatment with LGP. HSP70 was detected in naïve rat heart tissue extracts. I/R treatment significantly suppressed the level of HSP70 and increased the levels of CHIP in the heart. A complex of CHIP/HSP70 was detected in heart tissue extracts. The addition of recombinant CHIP to culture inhibited HSP70 in heart cells. LGP was bound CHIP in heart cells and prevented the CHIP from binding HSP70. In summary, I/R can suppress HSP70 and increase CHIP in heart cells. CHIP can suppress HSP70 that can be prevented by pretreatment with LGP. The results imply that CHIP may be a potential target in the prevention of I/R-induced heart cell injury.

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Crossref · 10.1007/s13105-012-0161-3

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Authors
Zhao, B., Sun, G., Feng, G., Duan, W., Zhu, X., Chen, S., Hou, L., Jin, Z., & Yi, D.
Affiliations
  • Xijing Hospital (CN)
  • Xian Central Hospital (CN)
Corresponding author
Jin, Zhenxiao
Venue
Journal of Physiology and Biochemistry
Published
2012-03-29
Volume
68
Issue
4
Pages
485-491
ISSN
  • 1138-7548 (ISSN-L)
  • 1877-8755
OpenAlex
W2122291185
Cited by
19
References
26
Citations by year
2026: 1 · 2025: 1 · 2023: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 3 · 2013: 1
FWCI
0.64
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Physiology and Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Cardiac Ischemia and Reperfusion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
Keywords
HSP70, CHIP, probiotics, myocardial ischemia-reperfusion injury, cardiac injury, cardioprotection, myocardial infarction
MeSH terms
Animals, Bacterial Proteins, Cardiotonic Agents, Cell Line, Male, Protein Binding, Myocardial Reperfusion Injury, Rats, Sprague-Dawley, HSP70 Heat-Shock Proteins, Myocytes, Cardiac, Ubiquitin-Protein Ligases, Rats, Proteolysis

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