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Influence of transgenic Bt176 and non-transgenic corn silage on the structure of rumen bacterial communities.
Open accessAbstract
The aim of the present study was to investigate the possible effects of a diet containing transgenic corn (Zea mays), cultivar Bt176 expressing the insecticidal protein CryIA(b), on bacterial diversity of the cow rumen in vivo. Silage prepared with Bt176 corn and its parental non-transgenic cultivar showed no significant differences in either composition or in vivo degradability. Four cows were fed for 35 days with the different silage in a cross-over feeding experiment. The overall structure of rumen bacterial populations was analyzed using a cultivation-independent approach—ribosomal intergenic spacer analysis (RISA)—during 3 consecutive days of each feeding trial in all cows. Planktonic bacterial populations present in the rumen fluid and biofilm grown directly on the silage were analyzed separately. Statistical analysis of RISA fingerprints did not reveal significant differences in bacterial community structure between different feedings. Small differences were nevertheless observed between individuals on the structure of bacterial communities directly colonizing the silage material. A diet constituted by transgenic Bt-corn silage was demonstrated not to be substantially different from non-transgenic corn silage in terms of composition, digestibility and impact on the overall rumen microbiota.
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Molecular tools such as DGGE, real-time PCR, TRFLP, RISA, and NGS have been used to uncover the epiphytic microbiota in fresh forages and microbiota in ensiled forages.
“In the past decade, molecular tools such as denaturing gradient gel electrophoresis (DGGE), real-time PCR, terminal restriction fragment length polymorphism (TRFLP), ribosomal intergenic spacer analysis (RISA), and next generation sequencing (NGS) have been used to uncover the epiphytic microbiota in fresh forages and microbiota in ensiled forages”
From Modulation of Metabolome and Bacterial Community in Whole Crop Corn Silage by Inoculating Homofermentative Lactobacillus plantarum and Heterofermentative Lactobacillus buchneri (2019) · cites this paper as Brusetti et al., 2011
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Metadata
- Authors
- Brusetti, L., Crotti, E., Tamburini, A., Cittaro, D., Garavaglia, V., Rolli, E., Sorlini, C., Daffonchio, D., & Borin, S.
- Affiliations
- Free University of Bozen-Bolzano (IT)
- University of Milan (IT)
- Corresponding author
- Borin, Sara
- ORCID
- Venue
- Annals of Microbiology
- Published
- 2011-02-12
- Volume
- 61
- Issue
- 4
- Pages
- 925-930
- ISSN
- 0003-4649 (ISSN-L)
- 1590-4261
- 1869-2044
- OpenAlex
- W2040641206
- Cited by
- 14
- References
- 39
- Citations by year
- 2022: 2 · 2020: 3 · 2019: 3 · 2017: 2 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 2.47
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Annals of Microbiology (publishedVersion)
- Open Research (University of Surrey) (submittedVersion)
- INFM-OAR (INFN Catania) (submittedVersion, other-oa)
- Funders
- Fondazione Cariplo
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- rumen microbiota, corn silage, dairy cattle, bacterioplankton, biofilm bacteria, RISA
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