CoChat
Sign Up
Cited by 30Biopolymer Synthesis and ApplicationsVitamin K Research StudiesEnzyme Production and Characterization

The DOI matches its Crossref record.

Coproduction of menaquinone-7 and nattokinase by Bacillus subtilis using soybean curd residue as a renewable substrate combined with a dissolved oxygen control strategy

Open access
Wang, Han, Sun, Xiaojuan, Wang, Li, Wu, Hefang, Zhao, Genhai, Liu, Hui, Wang, Peng, Zheng, Zhiming·2018·Annals of Microbiology
DOI 10.1007/s13213-018-1372-9
Open this paper in CoChat — free account

Abstract

Numerous physiological functions of menaquinone-7 (MK-7) act to reduce vascular calcification, suggesting that MK-7 may be a potential therapy for Alzheimer’s and Parkinson’s disease, and in this study, we attempted to increase the concentration of MK-7 synthesized by Bacillus subtilis natto, a standard nattokinase (NK) producing strain. Different Bacillus subtilis isolates demonstrated positive correlations between MK-7 and NK concentrations. Response surface methodology (RSM) was employed to optimize a culture medium for the simultaneous production of these molecules; the optimized medium contained the following components (%, w/v): soybean curd residue, 12.2; soya peptone, 5.7; lactose, 2.6; and K2HPO4, 0.6. The fermentation process was subsequently optimized based on online feedback control of fermentation process parameters. The dissolved oxygen (DO) concentration played an important role in the production of MK-7 and NK. With increased DO concentrations, the cell growth rate and NK activity increased. In contrast, at low DO concentrations, the concentration of MK-7 rapidly increased during the late fermentation stage. Thus, in this study, the production of MK-7 and NK by Bacillus subtilis was accomplished using soybean curd residue through medium optimization and DO control. This novel coproduction strategy was developed by controlling the aeration rate during the fermentation process. The concentrations of MK-7 and NK achieved in this study reached 91.25 mg/L and 2675.73 U/mL, respectively.

Overview

Integrity signals

Registry

Crossref · 10.1007/s13213-018-1372-9

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 42

  1. Anson ML (1938) The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. J Gen Physiol 22:79–89. https://doi.org/10.1085/jgp.22.1.79 · DOI
  2. Berenjian A et al (2011) Efficient media for high menaquinone-7 production: response surface methodology approach. New Biotechnol 28:665–672. https://doi.org/10.1016/j.nbt.2011.07.007 · DOI
  3. Berenjian A, Mahanama R, Talbot A, Regtop H, Kavanagh J, Dehghani F (2014) Designing of an intensification process for biosynthesis and recovery of Menaquinone-7. Appl Biochem Biotechnol 172:1347–1357. https://doi.org/10.1007/s12010-013-0602-7 · DOI
  4. Bhatt PC, Pathak S, Kumar V, Panda BP (2018) Attenuation of neurobehavioral and neurochemical abnormalities in animal model of cognitive deficits of Alzheimer's disease by fermented soybean nanonutraceutical. Inflammopharmacology 26:105–118. https://doi.org/10.1007/s10787-017-0381-9 · DOI
  5. Cai D, Zhu C, Chen S (2017) Microbial production of nattokinase: current progress, challenge and prospect. World J Microbiol Biotechnol 33. https://doi.org/10.1007/s11274-017-2253-2 · DOI
  6. Chen H, Xiao X, Wang J, Wu L, Zheng Z, Yu Z (2008) Antagonistic effects of volatiles generated by Bacillus subtilis on spore germination and hyphal growth of the plant pathogen, Botrytis cinerea. Biotechnol Lett 30:919–923. https://doi.org/10.1007/s10529-007-9626-9 · DOI
  7. Cho Y-H et al (2010) Production of nattokinase by batch and fed-batch culture of Bacillus subtilis. New Biotechnol 27:341–346. https://doi.org/10.1016/j.nbt.2010.06.003 · DOI
  8. Dabbagh F et al (2014) Nattokinase: production and application. Appl Microbiol Biotechnol 98:9199–9206. https://doi.org/10.1007/s00253-014-6135-3 · DOI
  9. Dajanta K, Chukeatirote E, Apichartsrangkoon A (2012) Improvement of thua nao production using protein-rich soybean and Bacillus subtilis TN51 starter culture. Ann Microbiol 62:785–795. https://doi.org/10.1007/s13213-011-0319-1 · DOI
  10. Dedavid e Silva LA, Macedo AJ, Termignoni C (2014) Production of keratinase by Bacillus subtilis S14. Ann Microbiol 64:1725–1733. https://doi.org/10.1007/s13213-014-0816-0 · DOI
  11. Deepak V, Kalishwaralal K, Ramkumarpandian S, Babu SV, Senthilkumar SR, Sangiliyandi G (2008) Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology. Bioresour Technol 99:8170–8174. https://doi.org/10.1016/j.biortech.2008.03.018 · DOI
  12. Garg R, Thorat BN (2014) Nattokinase purification by three phase partitioning and impact of t-butanol on freeze drying. Sep Purif Technol 131:19–26. https://doi.org/10.1016/j.seppur.2014.04.011 · DOI
  13. Hedstrom L (2002) Serine protease mechanism and specificity. Chem Rev 102:4501–4523. https://doi.org/10.1021/cr000033x · DOI
  14. Hu X-C, Liu W-M, Luo M-M, Ren L-J, Ji X-J, Huang H (2017) Enhancing Menaquinone-7 production by Bacillus natto R127 through the nutritional factors and surfactant. Appl Biochem Biotechnol. https://doi.org/10.1007/s12010-017-2423-6 · DOI
  15. Kirrolia A, Bishnoi NR, Singh R (2014) Response surface methodology as a decision-making tool for optimization of culture conditions of green microalgae chlorella spp. for biodiesel production. Ann Microbiol 64:1133–1147. https://doi.org/10.1007/s13213-013-0752-4 · DOI
  16. Kumar R, Mahajan S, Kumar A, Singh D (2011) Identification of variables and value optimization for optimum lipase production by Bacillus pumilus RK31 using statistical methodology. New Biotechnol 28:65–71. https://doi.org/10.1016/j.nbt.2010.06.007 · DOI
  17. Kwon E-Y, Kim KM, Kim MK, Lee IY, Kim BS (2011) Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis. Bioprocess Biosyst Eng 34:789–793. https://doi.org/10.1007/s00449-011-0527-x · DOI
  18. Li C, Xia JY, Chu J, Wang YH, Zhuang YP, Zhang SL (2013a) CFD analysis of the turbulent flow in baffled shake flasks. Biochem Eng J 70:140–150. https://doi.org/10.1016/j.bej.2012.10.012 · DOI
  19. Li S, Zhu D, Li K, Yang Y, Lei Z, Zhang Z (2013b) Soybean curd residue: composition, utilization, and related limiting factors. ISRN Ind Eng 2013:1–8. https://doi.org/10.1155/2013/423590 · DOI · Paper page
  20. Liu D et al (2015) Simultaneous production of butanol and acetoin by metabolically engineered clostridium acetobutylicum. Metab Eng 27:107–114. https://doi.org/10.1016/j.ymben.2014.11.002 · DOI
  21. Mandinia E, Demirci A, Berenjian A (2017) Production and application of menaquinone-7 (vitamin K2): a new perspective. World J Microbiol Biotechnol 33:7. https://doi.org/10.1007/s11274-016-2169-2 · DOI
  22. Meriem G, Mahmoud K (2017) Optimization of chitinase production by a new Streptomyces griseorubens C9 isolate using response surface methodology. Ann Microbiol 67:175–183. https://doi.org/10.1007/s13213-016-1249-8 · DOI
  23. Narasimhan MK, Chandrasekaran M, Rajesh M (2015) Fibrinolytic enzyme production by newly isolated Bacillus cereus SRM-001 with enhanced in-vitro blood clot lysis potential. J Gen Appl Microbiol 61:157–164. https://doi.org/10.2323/jgam.61.157 · DOI
  24. Ni H, Guo PC, Jiang WL, Fan XM, Luo XY, Li HH (2016) Expression of nattokinase in Escherichia coli and renaturation of its inclusion body. J Biotechnol 231:65–71. https://doi.org/10.1016/j.jbiotec.2016.05.034 · DOI
  25. Nikiforova OA, Klykov S, Volski A, Dicks LMT, Chikindas ML (2016) Subtilosin a production by Bacillus subtilis KATMIRA1933 and colony morphology are influenced by the growth medium. Ann Microbiol 66:661–671. https://doi.org/10.1007/s13213-015-1149-3 · DOI
  26. Reddy LV, Kim Y-M, Yun J-S, Ryu H-W, Wee Y-J (2016) L-lactic acid production by combined utilization of agricultural bioresources as renewable and economical substrates through batch and repeated-batch fermentation of Enterococcus faecalis RKY1. Bioresour Technol 209:187–194. https://doi.org/10.1016/j.biortech.2016.02.115 · DOI
  27. Sato T, Yamada Y, Ohtani Y, Mitsui N, Murasawa H, Araki S (2001) Production of menaquinone (vitamin K-2)-7 by Bacillus subtilis. J Biosci Bioeng 91:16–20. https://doi.org/10.1263/jbb.91.16 · DOI
  28. Sekar BS, Seol E, Park S (2017) Co-production of hydrogen and ethanol from glucose in Escherichia coli by activation of pentose-phosphate pathway through deletion of phosphoglucose isomerase (pgi) and overexpression of glucose-6-phosphate dehydrogenase (zwf) and 6-phosphogluconate dehydrogenase (gnd). Biotechnol Biofuels 10. https://doi.org/10.1186/s13068-017-0768-2 · DOI
  29. Sharma V, Meganathan R, Hudspeth MES (1993) Menaquinone (vitamin-K2) biosynthesis - cloning, nucleotide-sequence, and expression of the menc gene from escherichia-coli. J Bacteriol 175:4917–4921 · DOI
  30. Singh V, Haque S, Niwas R, Srivastava A, Pasupuleti M, Tripathi CK (2016) Strategies for fermentation medium optimization: an in-depth review. Front Microbiol 7:2087. https://doi.org/10.3389/fmicb.2016.02087 · DOI · Paper page
  31. Song J et al (2014) Enhanced production of vitamin K-2 from Bacillus subtilis (natto) by mutation and optimization of the fermentation medium. Braz Arch Biol Technol 57:606–612. https://doi.org/10.1590/s1516-8913201402126 · DOI
  32. Thadathil N, Kuttappan AKP, Vallabaipatel E, Kandasamy M, Velappan SP (2014) Statistical optimization of solid state fermentation conditions for the enhanced production of thermoactive chitinases by mesophilic soil fungi using response surface methodology and their application in the reclamation of shrimp processing by-products. Ann Microbiol 64:671–681. https://doi.org/10.1007/s13213-013-0702-1 · DOI
  33. Urano T et al (2001) The profibrinolytic enzyme subtilisin NAT purified from Bacillus subtilis cleaves and inactivates plasminogen activator inhibitor type 1. J Biol Chem 276:24690–24696. https://doi.org/10.1074/jbc.M101751200 · DOI
  34. Vos M, Verstreken P, Klein C (2015) Stimulation of electron transport as potential novel therapy in Parkinson's disease with mitochondrial dysfunction. Biochem Soc Trans 43:275–279. https://doi.org/10.1042/bst20140325 · DOI
  35. Vossen LM et al (2015) Menaquinone-7 supplementation to reduce vascular calcification in patients with coronary artery disease: rationale and study protocol (VitaK-CAC trial). Nutrients 7:8905–8915. https://doi.org/10.3390/nu7115443 · DOI
  36. Wang H, Xia J, Zheng Z, Zhuang YP, Yi X, Zhang D, Wang P (2018) Hydrodynamic investigation of a novel shear-generating device for the measurement of anchorage-dependent cell adhesion intensity. Bioprocess Biosyst Eng. https://doi.org/10.1007/s00449-018-1964-6 · DOI
  37. Wang S-L, Chen H-J, Liang T-W, Lin Y-D (2009) A novel nattokinase produced by Pseudomonas sp TKU015 using shrimp shells as substrate. Process Biochem 44:70–76. https://doi.org/10.1016/j.procbio.2008.09.009 · DOI
  38. Wei HF, Zhao GH, Liu H, Wang H, Ni WF, Wang P, Zheng ZM (2018) A simple and efficient method for the extraction and separation of menaquinone homologs from wet biomass of Flavobacterium. Bioprocess Biosyst Eng 41:107–113. https://doi.org/10.1007/s00449-017-1851-6 · DOI
  39. Xie MH, Xia JY, Zhou Z, Chu J, Zhuang YP, Zhang SL (2014) Flow pattern, mixing, gas hold-up and mass transfer coefficient of triple-impeller configurations in stirred tank bioreactors. Ind Eng Chem Res 53:5941–5953. https://doi.org/10.1021/ie400831s · DOI
  40. Xin B, Tao F, Wang Y, Liu HY, Ma CQ, Xu P (2017) Coordination of metabolic pathways: enhanced carbon conservation in 1,3-propanediol production by coupling with optically pure lactate biosynthesis. Metab Eng 41:102–114. https://doi.org/10.1016/j.ymben.2017.03.009 · DOI
  41. Xu Z et al (2015) Economic process to co-produce poly(epsilon-L-lysine) and poly(L-diaminopropionic acid) by a pH and dissolved oxygen control strategy. Bioresour Technol 187:70–76. https://doi.org/10.1016/j.biortech.2015.03.101 · DOI
  42. Zheng Z, Li W, Huang X, Qin W (2017) Effect of trace elements and optimization of their composition for the nitrification of a heterotrophic nitrifying bacterium, Acinetobacter harbinensis HITLi7(T), at low temperature. Ann Microbiol 67:715–725. https://doi.org/10.1007/s13213-017-1298-7 · DOI

Cited by

Sorted by citations

See all 30

Metadata

Authors
Wang, H., Sun, X., Wang, L., Wu, H., Zhao, G., Liu, H., Wang, P., & Zheng, Z.
Affiliations
  • University of Science and Technology of China (CN)
  • Chinese Academy of Sciences (CN)
Corresponding author
  • Wang, Peng
  • Zheng, Zhiming
ORCID
Venue
Annals of Microbiology
Publisher
Springer Science and Business Media LLC
Published
2018-09-14
Volume
68
Issue
10
Pages
655-665
ISSN
  • 0003-4649 (ISSN-L)
  • 1590-4261
  • 1869-2044
DOI
10.1007/s13213-018-1372-9
OpenAlex
W2891514938
Cited by
30
References
0
Citations by year
2026: 3 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 6 · 2019: 5
FWCI
1.25
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
Annals of Microbiology (publishedVersion)
Funders
  • The National High Technology Research and Development Program of China (2014AA021704)
  • Major Projects of Science and Technology in Anhui Province (17030801036)
Topics
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Biopolymer Synthesis and Applications
  • Health Sciences › Nursing › Nutrition and Dietetics › Vitamin K Research Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
Keywords
menaquinone-7, nattokinase, Bacillus natto, soybean curd residue, co-production, dissolved oxygen control, fermentation optimization, culture medium optimization, air flow rate, renewable substrates, vascular calcification, Alzheimer's disease, Parkinson's disease

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Coproduction of menaquinone-7 and nattokinase by Bacillus subtilis using soybean curd residue as a renewable substrate combined with a dissolved oxygen control strategy. https://app.cochat.ai/papers/10.1007/s13213-018-1372-9
The paper
Wang, H., Sun, X., Wang, L., Wu, H., Zhao, G., Liu, H., Wang, P., & Zheng, Z. (2018). Coproduction of menaquinone-7 and nattokinase by Bacillus subtilis using soybean curd residue as a renewable substrate combined with a dissolved oxygen control strategy. Annals of Microbiology. https://doi.org/10.1007/s13213-018-1372-9