Intraepithelial lymphocytes subsets in different forms of celiac disease
Open accessAbstract
AIM: The enumeration of intraepithelial lymphocytes subsets (total, γδ, and CD3(-) IELs) by flow cytometry (FCM), named as IEL lymphogram, constitutes a useful tool for celiac disease (CD) diagnosis. The aim of this study was to quantify IELs by FCM and their diagnostic value to differentiate active, silent and potential CD. METHODS: Prospective study of 60 active and 20 silent CD patients, and 161 controls in which duodenal biopsy and IEL quantification by FCM was performed. RESULTS: Active and silent CD patients had significant higher levels of both total and γδ IELs than absent CD patients (P < 0.0001 and P < 0.0001, P = 0.012 and P < 0.011; respectively). Active and silent CD patients had significant lower levels of CD3(-) IELs than absent CD patients (P < 0.047 and P < 0.009, respectively). Moreover, they were lower in silent than in active CD patients (P = 0.002). Changes of IELs subsets were more marked in children than adults active CD. The optimal IEL lymphogram cut off values for active CD diagnosis were: ≥10, ≥15 and ≤9 %, and with better performance characteristics for silent CD: ≥ 11, ≥10 and ≤5 %. CONCLUSION: The evaluation of IELs subsets by FCM is useful to confirm diagnosis of active and silent CD.
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The diagnostic efficacy of the assessment of iIELs by flow cytometry has been confirmed for the differentiation of active celiac disease patients, celiac disease in remission and subclinical or potential celiac disease in both pediatric and adult patients.
“The diagnostic efficacy of the assessment of iIELs by flow cytometry has been confirmed for the differentiation of active celiac disease patients, Celiac disease in remission and subclinical or potential celiac disease in both pediatric and adult patients [315-318]”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [317]
The Results report diagnostic performance of flow-cytometric IEL assessment for active and silent celiac disease, including separate analyses of children and adults with active disease. The Methods identify no remission group, and the Discussion says the authors lack conclusive data on potential celiac disease. Thus the source does not confirm the full range of differentiation claimed. (LLM, conf=0.89)
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- Authors
- Sánchez-Castañon, M., Castro, B. G., Toca, M., Santacruz, C., Arias-Loste, M., Iruzubieta, P., Crespo, J., & López-Hoyos, M.
- Affiliations
- Marqués de Valdecilla University Hospital (ES)
- Venue
- Autoimmunity Highlights
- Published
- 2016-09-23
- Volume
- 7
- Issue
- 1
- Pages
- 14-14
- ISSN
- 2038-0305 (ISSN-L)
- 2038-3274
- PMC
- PMC5035275
- OpenAlex
- W2521494116
- Cited by
- 23
- References
- 32
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 3 · 2018: 4 · 2017: 2
- FWCI
- 2.19
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Autoimmunity Highlights (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- IDIVAL (Lopez-Albo grant, CI 10/52, CI 13/10)
- Topics
- Health Sciences › Medicine › Gastroenterology › Celiac Disease Research and Management
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
- Health Sciences › Medicine › Surgery › Eosinophilic Esophagitis
- Keywords
- celiac disease, intraepithelial lymphocytes, γδ T cells, silent celiac disease, duodenal biopsy, diagnostic thresholds, pediatric celiac disease
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