Cited by 60Celiac Disease Research and ManagementEosinophilic EsophagitisInflammatory Bowel Disease

Dynamics of Non-conventional Intraepithelial Lymphocytes—NK, NKT, and γδ T—in Celiac Disease: Relationship with Age, Diet, and Histopathology

Calleja, Sara, Vivas, Santiago, Santiuste, María, Arias, Laura, Hernando, Mercedes, Nistal, Esther, Casqueiro, Javier, Ruiz de Morales, Jose G.·2011·Digestive Diseases and Sciences

Abstract

Background Intraepithelial lymphocytes (IEL) are a heterogeneous population of lymphocytes raised in celiac disease (CD), whose role in CD pathogenesis remains to be defined.Aims To investigate how the age of diagnosis, diet, and the severity of the histological lesions are related to the changes observed in unconventional IEL populations.Methods Prospective analysis of 101 confirmed celiac patients from a single center, including 66 at diagnosis (45 children, 21 adults) and 112 non-celiac controls (12 children, 100 adults). IEL from duodenal biopsies were studied by six-color flow cytometry. The results were analyzed in relationship with age, diet (gluten intake), and histopathology (Marsh type).Results In comparison with respective age controls, both children and adult patients showed duodenal intraepithelial lymphocytosis with significant differences in every single non-conventional IEL population: CD3+ TCR γδ, NK (CD3-, CD16+, CD56+), NKT (CD3+, CD161+, CD56+), and iNKT (CD3+ Vα24) (P < 0.001 for all). Gluten intake was not only directly associated with severe atrophy, but also with decreased percentages of NK (P = 0.02), NKT (P = 0.003), and iNKT (P = 0.03). Changes in iNKT and γδ IEL were more marked in celiac children compared with celiac adults (P = 0.02 and 0.01, respectively). In contrast, increased CD3+ TCR γδ were diet- and Marsh grade-independent.Conclusions The typical phenotypical profile of intraepithelial lymphocytosis in untreated pediatric and adult celiacs consists of increased CD3+ TCR γδ populations with decreased NK, NKT, and iNKT cells. NK, NKT, and iNKT IEL, but not γδ IEL, are dynamic populations associated with diet, age, and histopathology.

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Crossref · 10.1007/s10620-010-1534-5

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  • The diagnostic efficacy of the assessment of iIELs by flow cytometry has been confirmed for the differentiation of active celiac disease patients, celiac disease in remission and subclinical or potential celiac disease in both pediatric and adult patients.

    “The diagnostic efficacy of the assessment of iIELs by flow cytometry has been confirmed for the differentiation of active celiac disease patients, Celiac disease in remission and subclinical or potential celiac disease in both pediatric and adult patients [315-318]”

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Authors
Calleja, S., Vivas, S., Santiuste, M., Arias, L., Hernando, M., Nistal, E., Casqueiro, J., & Ruiz de Morales, J. G.
Affiliations
  • Hospital Universitario de León (ES)
  • Universidad de León (ES)
Venue
Digestive Diseases and Sciences
Published
2011-01-08
Volume
56
Issue
7
Pages
2042-2049
ISSN
  • 0163-2116 (ISSN-L)
  • 1573-2568
OpenAlex
W2040610974
Cited by
60
References
37
Citations by year
2026: 2 · 2024: 2 · 2023: 4 · 2022: 4 · 2021: 7 · 2020: 5 · 2019: 7 · 2018: 6 · 2017: 2 · 2016: 3 · 2015: 7 · 2014: 3 · 2013: 3 · 2012: 4
FWCI
3.59
Type
journal article
Language
en
Open access
closed
Locations
  • Digestive Diseases and Sciences (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Junta de Castilla y León
  • Instituto de Salud Carlos III
Topics
  • Health Sciences › Medicine › Gastroenterology › Celiac Disease Research and Management
  • Health Sciences › Medicine › Surgery › Eosinophilic Esophagitis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
Keywords
celiac disease, intraepithelial lymphocytes, natural killer cells, NKT cells, invariant natural killer T cells, γδ T cells, intraepithelial lymphocytosis, gluten intake, duodenal histopathology, age at diagnosis, pediatric celiac disease, duodenal biopsy
MeSH terms
Adolescent, Adult, Age Factors, Aged, Celiac Disease, Child, Child, Preschool, Diet, Duodenum, Female, Glutens, Humans, Infant, Intestinal Mucosa, Killer Cells, Natural, Lymphocytosis, Male, Middle Aged, Receptors, Antigen, T-Cell, gamma-delta, Natural Killer T-Cells, Young Adult

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