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Sensitive substrates for human leukocyte and porcine pancreatic elastase: A study of the merits of various chromophoric and fluorogenic leaving groups in assays for serine proteases
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“The KM for MeOSuc-AAPV-pNA was found to be 0.152 mM compared to a previously published value of 0.14 mM [29].”
From Discrimination of Methionine Sulfoxide and Sulfone by Human Neutrophil Elastase (2021) · cites this paper as [29]
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Kakegawa, H., Matano, Y., Inubushi, T., & Katunuma, N. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.01.176 · Cited by 11
Metadata
- Authors
- Castillo, M. J., Nakajima, K., Zimmerman, M., & Powers, J. C.
- Affiliations
- Georgia Institute of Technology (US)
- Merck & Co., Inc., Rahway, NJ, USA (United States) (US)
- Corresponding author
- Powers, James C.
- Venue
- Analytical Biochemistry
- Publisher
- Elsevier BV
- Published
- 1979-10
- Volume
- 99
- Issue
- 1
- Pages
- 53-64
- ISSN
- 0003-2697 (ISSN-L)
- OpenAlex
- W2059777229
- Cited by
- 268
- References
- 28
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 6 · 2016: 3 · 2015: 1 · 2014: 4 · 2013: 3 · 2012: 3
- FWCI
- 5.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Peptidase Inhibition and Analysis
- Keywords
- elastase, neutrophil elastase, porcine pancreatic elastase, fluorogenic substrates, chromogenic substrates, 4-methylcoumarin, Ellman's reagent, 4,4'-dithiodipyridine, enzyme kinetics, substrate sensitivity
- MeSH terms
- Amino Acid Sequence, Animals, Humans, Indicators and Reagents, Kinetics, Leukocytes, Oligopeptides, Pancreas, Pancreatic Elastase, Peptide Hydrolases, Serine, Spectrometry, Fluorescence, Substrate Specificity, Swine
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