In vitro reactivation of in vivo ammonium-inactivated glutamine synthetase from Synechocystis sp. PCC 6803
Abstract
Glutamine synthetase from Synechocystis sp. strain PCC 6803 is inactivated by ammonium addition to cells growing with nitrate as the nitrogen source. The enzyme can be reactivated in vitro by different methods such as alkaline phosphatase treatment, but not phosphodiesterase, by raising the pH of the crude extract to values higher than 8, by increasing the ionic strength of the cell-free extract, or by preincubation with organic solvents, such as 2-propanol and ethanol. These results suggest that the loss of glutamine synthetase activity promoted by ammonium involves the non-covalent binding of a phosphorylated compound to the enzyme and support previous results that rule out the existence of an adenylylation/deadenylylation system functioning in the regulation of cyanobacterial glutamine synthetase.
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2-oxoglutarate plays a fundamental role in amino acid biosynthesis.
“It has been suggested that 2-OG plays a fundamental role in the amino acid biosynthesis. This theory has been supported in Synechocystis by experiments where the external addition of ammonium to N2-free medium enhanced the 2-OG levels as well as the glutamate and glutamine production (Merida et al. 1991).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Merida et al. 1991
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Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering
Quintana, N., Van der Kooy, F., Van de Rhee, M. D., Voshol, G. P., & Verpoorte, R. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3394-0 · Cited by 338 · see citing works
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Self-citation
- Authors
- Mérida, A., Candau, P., & Florencio, F. J.
- Affiliations
- Instituto de Bioquímica Vegetal y Fotosíntesis (ES)
- Corresponding author
- Florencio, F.J.
- ORCID
- Florencio, F.J.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1991-12
- Volume
- 181
- Issue
- 2
- Pages
- 780-786
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2071762067
- Cited by
- 23
- References
- 16
- Citations by year
- 2025: 1 · 2018: 1 · 2015: 1
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- glutamine synthetase, Synechocystis sp. PCC 6803, enzyme reactivation, alkaline phosphatase, enzyme regulation
- MeSH terms
- Cyanobacteria, Alkaline Phosphatase, Ammonia, Enzyme Activation, Enzyme Reactivators, Glutamate-Ammonia Ligase, Hydrogen-Ion Concentration, Kinetics, Osmolar Concentration, Potassium Chloride
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