The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Interleukin-10 (IL-10) inhibits the induction of nitric oxide synthase by interferon-γ in murine macrophages
Abstract
A murine macrophage cell line, J774, expresses high levels of the enzyme nitric oxide synthase (NOS) and produces large amounts of nitric oxide (NO) when activated with recombinant interferon (IFN)-gamma and a low concentration of LPS (10 ng/ml). Both the expression of NOS and the production of NO were inhibited by recombinant IL-10 in a dose-dependent manner. The inhibition was effective only when the cells were pretreated with IL-10; addition of IL-10 at the same time or after IFN-gamma activation was without effect. These results demonstrate that IL-10, a product of Th2 (helper T lymphocyte 2) cells, can antagonise the function of IFN-gamma, a product of Th1 cells, by modulating the mechanism of synthesis of nitric oxide in the macrophages.
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“Macrophages themselves are affected by IL-10 in that exposure to this cytokine lowers their microbicidal activity and diminishes their capacity to respond to IFN-γ (63, 64).”
From Macrophage Cytokines: Involvement in Immunity and Infectious Diseases (2014) · cites this paper as [64]
In the citing paper
“NO also increases levels of the anti-inflammatory cytokine interleukin-10 (IL-10) in Treg cells as well as in some cancer cells [63-65].”
From Signaling and stress: The redox landscape in NOS2 biology (2015) · cites this paper as [65]
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Metadata
- Authors
- Cunha, F. Q., Mohcada, S., & Liew, F. Y.
- Affiliations
- Wellcome Trust (GB)
- University of Glasgow (GB)
- Corresponding author
- Liew, F.Y.
- ORCID
- Cunha, F.Q.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1992-02
- Volume
- 182
- Issue
- 3
- Pages
- 1155-1159
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2038992843
- Cited by
- 383
- References
- 23
- Citations by year
- 2026: 4 · 2023: 3 · 2022: 2 · 2021: 6 · 2020: 4 · 2019: 5 · 2018: 8 · 2017: 3 · 2016: 5 · 2015: 5 · 2014: 7 · 2013: 6 · 2012: 5
- FWCI
- 11.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- IL-10, interferon-gamma, nitric oxide synthase, nitric oxide, macrophages, J774 cells, lipopolysaccharide, Th1/Th2 balance, cytokine antagonism, macrophage activation
- MeSH terms
- Amino Acid Oxidoreductases, Animals, Cell Line, Enzyme Induction, Interferon-gamma, Kinetics, Macrophages, Nitric Oxide, Recombinant Proteins, Interleukin-10, Nitric Oxide Synthase, Mice
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