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Cited by 63Heat shock proteins researchThermoregulation and physiological responsesEndoplasmic Reticulum Stress and Disease

HSP72 induction by heat stress in human neurons and glial cells in culture

Satoh, Jun-ichi, Kim, Seung U.·1994·Brain Research
DOI 10.1016/0006-8993(94)90396-4
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Abstract

Expression of 72-kDa heat shock protein (HSP72) induced by heat stress was investigated in cultured neurons and glial cells isolated from fetal human brains using immunoblotting and immunocytochemistry. Under the unstressed condition, a low level of HSP72 expression was observed in astrocytes, microglia, oligodendrocytes, and neurons. Under the heat-stressed condition, an increased expression of HSP72 was observed in all cell types with specific location in the nuclear and cytoplasmic regions. Following heat stress, HSP72 was expressed intensely in more than 50% of astrocytes and microglia during 8-24 h post-recovery, while it was detectable in only 9% of oligodendrocytes and 3% of neurons at 48 h post-recovery. These results indicate that heat stress induces a predominant expression of HSP72 in astrocytes and microglia, and more limited HSP72 expression in oligodendrocytes and neurons in fetal human neural cells in culture. The differential patterns of HSP72 induction in human neural cells by heat stress suggest that cellular mechanisms by which the heat shock response is regulated are different among various cell types in the human central nervous system.

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Crossref · 10.1016/0006-8993(94)90396-4

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48 of 48 references verified0 citation errors · 0 not checkable

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  • Microglial reaction to heat stress is significantly faster than for other CNS cell types.

    “These results are in agreement with previous in vitro work which found that microglial reaction to heat stress was significantly faster than for other CNS cell types35, although that does not preclude such artifacts being present in other cell types, depending on the details of cell isolation33.”

    From Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain (2022) · cites this paper as [35]

  • Induction of the ex vivo signature occurred only in microglia and other brain-associated myeloid cells, while other cell types may show initial signs of this response (not significant).

    “In line with previous work on the response of CNS cells to stress35, we found that induction of this ex vivo signature occurred only in microglia and other brain-associated myeloid cells, but, while not significant, we found that other cell types may show initial signs of this response, which could become significant under different conditions.”

    From Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain (2022) · cites this paper as [35]

  • The weaker astrocyte enrichment suggests differential time scales of activation for microglia and astrocytes.

    “suggestive of differential time scales of activation for these two brain populations, as has been previously described35.”

    From Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain (2022) · cites this paper as [35]

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Authors
Satoh, J.-I., & Kim, S. U.
Affiliations
University of British Columbia Hospital (CA)
Corresponding author
Kim, Seung U.
Venue
Brain Research
Publisher
Elsevier BV
Published
1994-08
Volume
653
Issue
1-2
Pages
243-250
ISSN
0006-8993 (ISSN-L)
DOI
10.1016/0006-8993(94)90396-4
OpenAlex
W2067749913
Cited by
63
References
48
Citations by year
2022: 1 · 2019: 1 · 2018: 2 · 2014: 1 · 2012: 1
FWCI
1.39
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Multiple Sclerosis Society
  • Fondation pour la Recherche Médicale
  • Medical Research Council Canada
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Health Sciences › Medicine › Physiology › Thermoregulation and physiological responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Endoplasmic Reticulum Stress and Disease
Keywords
HSP72, HSP70, heat stress, heat shock response, astrocytes, microglia, oligodendrocytes, neurons, cell type-specific gene expression, central nervous system
MeSH terms
Astrocytes, Brain, Cells, Cultured, Fetus, Hot Temperature, Heat-Shock Proteins, Humans, Immunohistochemistry, Neuroglia, Neurons, Oligodendroglia, Stress, Physiological, Immunoblotting, HSP72 Heat-Shock Proteins

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