Cited by 557Antioxidant Activity and Oxidative StressGlutathione Transferases and PolymorphismsRedox biology and oxidative stress

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A simplified assay method of superoxide dismutase activity for clinical use

Masayasu, Minami, Hiroshi, Yoshikawa·1979·Clinica Chimica Acta

DOI 10.1016/0009-8981(79)90211-0

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Abstract

A simple and inexpensive assay method of superoxide dismutase (SOD) activity of various biological materials has been developed. SOD activity can easily be measured by reading the medium's intense absorbance directly, and many samples can be treated in a short time using a reaction stopper which acts for a long time. Reproducibility of the assay method was excellent for the sample from an individual estimated at two different times (a week to a month) and the coefficient of variance was 5.1%. The mean and standard deviation of SOD activities of 45 blood samples from normal Japanese males 32.9 +/- 10.5 microgram per ml of blood; estimated using bovine SOD standard. The SOD activity of the fluid from lavaged lungs was 15.7 +/- 1.8 microgram/ml, and that of lung tissue homogenates from rats was 2.85 +/- 0.66 microgram/mg of protein.

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Authors: Masayasu, M., & Hiroshi, Y.
Corresponding author: Masayasu, Minami
Venue: Clinica Chimica Acta
Publisher: Elsevier BV
Published: 1979-03
Volume: 92
Issue: 3
Pages: 337-342
ISSN: 0009-8981 (ISSN-L)
DOI: 10.1016/0009-8981(79)90211-0
OpenAlex: W2012484566
Cited by: 557
References: 17
Citations by year: 2026: 14 · 2025: 29 · 2024: 28 · 2023: 22 · 2022: 29 · 2021: 22 · 2020: 24 · 2019: 33 · 2018: 24 · 2017: 27 · 2016: 23 · 2015: 25 · 2014: 24 · 2013: 24 · 2012: 17
FWCI: 3.71
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Clinica Chimica Acta (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords: superoxide dismutase, lung lavage fluid, assay reproducibility
MeSH terms: Adult, Animals, Colorimetry, Female, Humans, Lung, Male, Middle Aged, Superoxide Dismutase, Rats

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A simplified assay method of superoxide dismutase activity for clinical use

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