Cited by 12,892Redox biology and oxidative stressGlutathione Transferases and PolymorphismsMetal-Catalyzed Oxygenation Mechanisms

Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels

Beauchamp, Charles, Fridovich, Irwin·1971·Analytical Biochemistry

Integrity signals

Registry

Crossref · 10.1016/0003-2697(71)90370-8

Cites

21 of 21 references verified0 citation errors · 0 not checkable

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  • In some assays, O2 reduces tetranitro methane, cytochrome c or nitro tetrazolium blue, and in others it oxidises epinephrine, tiron pyrogallol or 6-hydroxydopamine.

    “Thus, in some assays, Oz reduces tetranitro methane [11], cytochrome c or nitro tetrazolium blue [100], and in others it oxidises epinephrine, tiron pyrogallol or 6-hydroxydopamine [101,102], Figure 38.”

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Authors
Beauchamp, C., & Fridovich, I.
Affiliations
Duke Medical Center (US)
Venue
Analytical Biochemistry
Publisher
Elsevier BV
Published
1971-11
Volume
44
Issue
1
Pages
276-287
ISSN
0003-2697 (ISSN-L)
OpenAlex
W2101422765
Cited by
12,892
References
21
Citations by year
2026: 577 · 2025: 678 · 2024: 591 · 2023: 617 · 2022: 654 · 2021: 674 · 2020: 577 · 2019: 535 · 2018: 500 · 2017: 463 · 2016: 436 · 2015: 467 · 2014: 500 · 2013: 435 · 2012: 429
FWCI
11.16
Type
journal article
Language
en
Open access
closed
Locations
  • Analytical Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
  • Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
Keywords
superoxide dismutase, nitroblue tetrazolium, superoxide, enzyme activity assay, polyacrylamide gel electrophoresis, enzyme localization, Escherichia coli, bovine tissues, enzyme stoichiometry
MeSH terms
Animals, Brain, Cattle, Electrophoresis, Disc, Erythrocytes, Escherichia coli, Free Radicals, Indicators and Reagents, Kinetics, Lung, Methods, Myocardium, Oxidation-Reduction, Oxidoreductases, Oxygen, Photochemistry, Spectrophotometry, Tetrazolium Salts, Xanthine Oxidase

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