Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels
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In some assays, O2 reduces tetranitro methane, cytochrome c or nitro tetrazolium blue, and in others it oxidises epinephrine, tiron pyrogallol or 6-hydroxydopamine.
“Thus, in some assays, Oz reduces tetranitro methane [11], cytochrome c or nitro tetrazolium blue [100], and in others it oxidises epinephrine, tiron pyrogallol or 6-hydroxydopamine [101,102], Figure 38.”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [100]
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Self-citation
- Authors
- Beauchamp, C., & Fridovich, I.
- Affiliations
- Duke Medical Center (US)
- Venue
- Analytical Biochemistry
- Publisher
- Elsevier BV
- Published
- 1971-11
- Volume
- 44
- Issue
- 1
- Pages
- 276-287
- ISSN
- 0003-2697 (ISSN-L)
- OpenAlex
- W2101422765
- Cited by
- 12,892
- References
- 21
- Citations by year
- 2026: 577 · 2025: 678 · 2024: 591 · 2023: 617 · 2022: 654 · 2021: 674 · 2020: 577 · 2019: 535 · 2018: 500 · 2017: 463 · 2016: 436 · 2015: 467 · 2014: 500 · 2013: 435 · 2012: 429
- FWCI
- 11.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
- Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
- Keywords
- superoxide dismutase, nitroblue tetrazolium, superoxide, enzyme activity assay, polyacrylamide gel electrophoresis, enzyme localization, Escherichia coli, bovine tissues, enzyme stoichiometry
- MeSH terms
- Animals, Brain, Cattle, Electrophoresis, Disc, Erythrocytes, Escherichia coli, Free Radicals, Indicators and Reagents, Kinetics, Lung, Methods, Myocardium, Oxidation-Reduction, Oxidoreductases, Oxygen, Photochemistry, Spectrophotometry, Tetrazolium Salts, Xanthine Oxidase
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