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A simplified assay method of superoxide dismutase activity for clinical use
Abstract
A simple and inexpensive assay method of superoxide dismutase (SOD) activity of various biological materials has been developed. SOD activity can easily be measured by reading the medium's intense absorbance directly, and many samples can be treated in a short time using a reaction stopper which acts for a long time. Reproducibility of the assay method was excellent for the sample from an individual estimated at two different times (a week to a month) and the coefficient of variance was 5.1%. The mean and standard deviation of SOD activities of 45 blood samples from normal Japanese males 32.9 +/- 10.5 microgram per ml of blood; estimated using bovine SOD standard. The SOD activity of the fluid from lavaged lungs was 15.7 +/- 1.8 microgram/ml, and that of lung tissue homogenates from rats was 2.85 +/- 0.66 microgram/mg of protein.
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“In the method described by Minami and Yoshikawa, 1979, the generation of the superoxide radical is produced by the chemical autoxidation of pyrogallol at pH = 8.2 [104], Figure 39.”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [104]
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Metadata
- Authors
- Masayasu, M., & Hiroshi, Y.
- Corresponding author
- Masayasu, Minami
- Venue
- Clinica Chimica Acta
- Publisher
- Elsevier BV
- Published
- 1979-03
- Volume
- 92
- Issue
- 3
- Pages
- 337-342
- ISSN
- 0009-8981 (ISSN-L)
- OpenAlex
- W2012484566
- Cited by
- 557
- References
- 17
- Citations by year
- 2026: 14 · 2025: 29 · 2024: 28 · 2023: 22 · 2022: 29 · 2021: 22 · 2020: 24 · 2019: 33 · 2018: 24 · 2017: 27 · 2016: 23 · 2015: 25 · 2014: 24 · 2013: 24 · 2012: 17
- FWCI
- 3.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinica Chimica Acta (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- superoxide dismutase, lung lavage fluid, assay reproducibility
- MeSH terms
- Adult, Animals, Colorimetry, Female, Humans, Lung, Male, Middle Aged, Superoxide Dismutase, Rats
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