Species differences in the concentrations and distributions of opioid binding sites
Abstract
Binding at the mu, delta- and kappa-types of opioid binding sites was compared in homogenates from the brains of guinea-pig, rabbit, rat and two mouse strains, under conditions of selective labelling. Species differences were shown by two observations. Firstly, analysis of saturation curves in homogenates of brain from which the cerebellum had been removed showed that in guinea-pig brain the opioid binding sites consist of 24% mu-sites, 32% delta-sites and 44% kappa-sites. In contrast, in rabbit brain the corresponding values are 43% mu-sites, 19% delta-sites and 37% kappa-sites and in rat brain, 46% mu-sites, 42% delta-sites and 12% kappa-sites. In the brains of DBA/2 mice the opioid binding sites are comprised of 51% mu-sites, 29% delta-sites and 20% kappa-sites and in C57BL/10 mice, of 44% mu-sites, 35% delta-sites and 21% kappa-sites; these strain differences are due to significant differences in the concentrations of the mu-sites. Secondly, species differences were found when the binding of single concentrations of tritiated ligands (1 X KD value in whole brain) was determined at mu-, delta- and kappa-sites in six brain regions from guinea-pig, rat or rabbit.
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The three opioid receptor types have distinct patterns of expression throughout the brain, with significant inter-species variability.
“These three receptor types have distinct patterns of expression throughout the brain, and these patterns have significant inter-species variability (Khachaturian et al 1985; Robson et al 1985; Mansour et al 1987; Mansour et al 1988; Mansour et al 1994a; Mansour et al 1994b; Curran and Watson 1995; Resendez et al 2012).”
From The behavioral, anatomical and pharmacological parallels between social attachment, love and addiction (2012) · cites this paper as Robson et al 1985
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Uniform assessment and ranking of opioid Mu receptor binding constants for selected opioid drugs
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“These three receptor types have distinct patterns of expression throughout the brain, and these patterns have significant interspecies variability (Khachaturian et al. 1985; Robson et al. 1985; Mansour et al. 1987, 1988, 1994a, b; Curran and Watson 1995; Resendez et al. 2012).”
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“This distribution pattern is similar to that of other rodents (guinea pig, rabbit, mouse, and rat) that also show dense binding within the striatum (Robson et al., 1985; Wang et al., 2011).”
BackgroundResultInfluential
- Authors
- Robson, L. E., Gillan, M. G. C., & Kosterlitz, H. W.
- Affiliations
- University of Aberdeen (GB)
- Corresponding author
- Robson, Linda E.
- Venue
- European Journal of Pharmacology
- Publisher
- Elsevier BV
- Published
- 1985-05
- Volume
- 112
- Issue
- 1
- Pages
- 65-71
- ISSN
- 0014-2999 (ISSN-L)
- OpenAlex
- W2021213716
- Cited by
- 101
- References
- 24
- Citations by year
- 2026: 2 · 2025: 1 · 2021: 2 · 2020: 2 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 3
- FWCI
- 3.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Medical Research Council
- National Institute on Drug Abuse (DA-00662)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- opioid binding sites, mu-opioid receptor, delta opioid receptor, kappa opioid receptor, species differences, strain differences, DBA/2 mice, C57BL/10 mice, guinea pig, rats, rabbits, brain regional distribution, receptor binding affinity, cerebellum
- MeSH terms
- Animals, Binding Sites, Brain, Guinea Pigs, Hypothalamus, Mice, Inbred C57BL, Mice, Inbred DBA, Rabbits, Receptors, Opioid, Species Specificity, Receptors, Opioid, mu, Receptors, Opioid, delta, Receptors, Opioid, kappa, Mice, Rats, In Vitro Techniques
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