The relative proportions of lysosomal enzyme activities in bovine retinal pigment epithelium
Abstract
The lysosome fractions from bovine retina, liver and retinal pigment epithelium were isolated by subcellular fractionation and compared with regard to the relative proportions of several hydrolytic enzyme activities. It was found that the lysosome fraction of the retinal pigment epithelium is more than three times as active as the lysosome fractions from other tissues in degrading the rhodopsin of photoreceptor (rod) cell outer segments. This proteolytic activity is attributable to a cathepsin D-like proteinase, and the possible biochemical bases for its increased activity in the pigment epithelium are discussed, including interaction with phospholipase A. It is suggested that the lysosomes of the retinal pigment epithelium are specialized in their content of hydrolytic enzymes for the degradation of photoreceptor cell outer segments.
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Phospholipase A activity in the RPE was reported in the early 1980s.
“Phospholipase A activity in the RPE was reported in the early 1980s (Zimmerman et al., 1983).”
From The cell biology of the retinal pigment epithelium (2020) · cites this paper as Zimmerman et al., 1983
RPE lysosomes are 7-fold more potent than liver lysosomes in degrading OS proteins.
“RPE lysosomes are 7-fold more potent than liver lysosomes in degrading OS proteins (Zimmerman et al., 1983).”
From The cell biology of the retinal pigment epithelium (2020) · cites this paper as Zimmerman et al., 1983
Cites
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The cell biology of the retinal pigment epithelium
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- Authors
- Zimmerman, W. F., Godchaux, W., & Belkin, M.
- Affiliations
- Amherst College (US)
- Venue
- Experimental Eye Research
- Publisher
- Elsevier BV
- Published
- 1983-01
- Volume
- 36
- Issue
- 1
- Pages
- 151-158
- ISSN
- 0014-4835 (ISSN-L)
- OpenAlex
- W2027244179
- Cited by
- 46
- References
- 18
- Citations by year
- 2021: 1 · 2020: 3 · 2015: 1 · 2014: 2 · 2012: 1
- FWCI
- 0.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Eye Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- lysosomes, retinal pigment epithelium, lysosomal enzymes, hydrolytic enzymes, photoreceptor outer segments, cathepsin D, proteolysis, phospholipase A, bovine retina, subcellular fractionation
- MeSH terms
- Animals, Cattle, Hydrolases, Liver, Lysosomes, Pigment Epithelium of Eye, Retina
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