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Cited by 21Parasitic Diseases Research and TreatmentParasites and Host InteractionsHelminth infection and control

Dirofilaria immitis: Biochemical and immunological characterization of the surface antigens from adult parasites

Scott, Alan L, Diala, Chamberlain, Moraga, David A, Ibrahim, M.S, Redding, Lydia, Tamashiro, Wesley K·1988·Experimental Parasitology
DOI 10.1016/0014-4894(88)90078-1
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Abstract

The molecules associated with the surface of adult Dirofilaria immitis were identified and characterized employing IODO-GEN-mediated surface labeling methods. D. immitis female and male parasites were found to have a limited number of surface-associated proteins (17.5, 16, and 14.5 kDa) and glycoproteins (49 and 20 kDa) which were readily extracted from parasite homogenates in the absence of detergent. The major surface labeled proteins and glycoproteins were antigenic in rabbits, but appeared to elicit only a weak humoral response in dogs with patent dirofilariasis. In addition, a 10- to 6-kDa surface-associated glycolipid was identified which may form a coat on the outside of the parasite and play a role in immune evasion. In immunoprecipitation experiments, the glycolipid was not recognized by the antibodies from rabbits exposed to the glycolipid or by antibodies in the sera of patently infected animals. The glycolipid and the 14.5-kDa surface protein were selectively released by the adult parasite during in vitro culture.

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Crossref · 10.1016/0014-4894(88)90078-1

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43 of 43 references verified0 citation errors · 0 not checkable

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  • A nonimmunogenic 6- to 10-kDa glycolipid on the surface of adult D. immitis worms may constitute a barrier against protease activity at the epicuticle-host interface.

    “A nonimmunogenic 6-to 10-kDa glycolipid associated with the surface of adult worms may constitute a barrier against protease activity at the epicuticle-host interface, also contributing to the evasion mechanisms (381).”

    From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [381]

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Authors
Scott, A. L., Diala, C., Moraga, D. A., Ibrahim, M. S., Redding, L., & Tamashiro, W. K.
Affiliations
Johns Hopkins University (US)
ORCID
Venue
Experimental Parasitology
Publisher
Elsevier BV
Published
1988-12
Volume
67
Issue
2
Pages
307-323
ISSN
0014-4894 (ISSN-L)
DOI
10.1016/0014-4894(88)90078-1
OpenAlex
W2070341223
Cited by
21
References
43
Citations by year
2019: 1 · 2016: 1 · 2012: 1
FWCI
2.02
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Experimental Parasitology (publishedVersion)
  • PubMed (publishedVersion)
Funders
U.S. Public Health Service (T32 A 107057)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
  • Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
  • Health Sciences › Veterinary › Small Animals › Helminth infection and control
Keywords
Dirofilaria immitis, surface glycoprotein, surface-associated proteins, immune evasion, humoral immune response, antigenic proteins
MeSH terms
Animals, Epitopes, Antigens, Helminth, Antigens, Surface, Dirofilaria immitis, Dogs, Electrophoresis, Polyacrylamide Gel, Female, Filarioidea, Glycolipids, Glycoproteins, Hydrogen-Ion Concentration, Indicators and Reagents, Male, Molecular Weight, Peptides, Precipitin Tests, Rabbits, Urea

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