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Separation and characterization of two extracelluar H2O2‐dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium
Open accessAbstract
Two H 2 O 2‐dependent oxidases found in the extracellular medium of the white rot fungus Phanerochaete chrysosporium were separated by chromatography on blue agarose. The first enzyme fraction to elute from the column generated ethylene from 2‐keto‐4‐thiomethylbutyric acid (KTBA) in the presence of veratryl alcohol, and catalyzed the α,β cleavage of the diarylpropane 1‐(3,4‐diethoxyphenyl)1,3‐dihdyroxy‐(4‐methoxyphenyl)propane (I). During the diarylpropane cleavage, 18 O from 18 O 2 was incorporated specifically into the α‐position of the product 1‐(4‐methoxyphenyl)1,2‐dihdyroxyethane (III), suggesting that this enzyme is an H 2 O 2‐dependent oxygenase. The second enzyme which binds to blue agarose is an Mn 2+‐dependent, lactate‐activated peroxidase. The enzyme catalyzed the oxidation of phenol red, o‐dianisidine, Poly R, and a variety of other dyes. It was also capable of decarboxylating vanillic acid.
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Cited by
[1] Role of free radicals and catalytic metal ions in human disease: An overview
Halliwell, B., & Gutteridge, J. M. C. (1990) · Methods in Enzymology · doi:10.1016/0076-6879(90)86093-b · Cited by 4,994
The Catalytic Valorization of Lignin for the Production of Renewable Chemicals
Zakzeski, J., Bruijnincx, P. C. A., Jongerius, A. L., & Weckhuysen, B. M. (2010) · Chemical Reviews · doi:10.1021/cr900354u · Cited by 4,468
The importance of free radicals and catalytic metal ions in human diseases.
Halliwell, B., & Gutteridge, J. M. C. (1985) · Molecular Aspects of Medicine · doi:10.1016/0098-2997(85)90001-9 · Cited by 941
Ligninolytic enzymes and its mechanisms for degradation of lignocellulosic waste in environment
Kumar, A., & Chandra, R. (2020) · Heliyon · doi:10.1016/j.heliyon.2020.e03170 · Cited by 648
A promising laccase immobilization approach for Bisphenol A removal from aqueous solutions
Lassouane, F., Aït-Amar, H., Amrani, S., & Rodriguez-Couto, S. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2018.09.129 · Cited by 251
Metadata
- Authors
- Kuwahara, M., Glenn, J. K., Morgan, M. A., & Gold, M. H.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1984-04-24
- Volume
- 169
- Issue
- 2
- Pages
- 247-250
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2040819974
- Cited by
- 792
- References
- 0
- FWCI
- 25.40
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Separation and characterization of two extracelluar H2O2‐dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. https://app.cochat.ai/papers/10.1016/0014-5793(84)80327-0
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- Kuwahara, M., Glenn, J. K., Morgan, M. A., & Gold, M. H. (1984). Separation and characterization of two extracelluar H2O2‐dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Letters. https://doi.org/10.1016/0014-5793(84)80327-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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