An NAD‐specific glutamate dehydrogenase from cyanobacteria Identification and properties
Open accessAbstract
The unicellular cyanobacterium Synechocystis sp. PCC 6803 presents a hexameric NAD‐specific glutamate dehydrogenase with a molecular mass of 295 kDa. The enzyme differs from the NADP‐glutamate dehydrogenase found in the same strain and is coded by a different gene. NAD‐glutamate dehydrogenase shows a high coenzyme specificity, catalyzes preferentially glutamate formation and presents K m values for ammonium, NADH and 2‐oxoglutarate of 4.5 mM, 50 μM and 1.8 mM respectively. An animating role for the enzyme is discussed.
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In cyanobacteria, 2-oxoglutarate is directly involved in N2 fixation via the NH3-assimilating mechanisms GDH and GS-GOGAT.
“In these microorganisms, 2-OG is directly involved in the N2 fixation via the NH3-assimilating mechanisms, GDH (gdhA, EC 1.4.1.14) (Chávez and Candau 1991) and GS-GOGAT route (Meeks et al. 1978).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Chávez and Candau 1991
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Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Role and regulation of L-glutamate dehydrogenase activity in higher plants
Srivastava, H. S., & Singh, R. P. (1987) · Phytochemistry · doi:10.1016/s0031-9422(00)84749-4 · Cited by 248 · see citing works
Smith, E. L., Austen, B., Blumenthal, K. M., et al. (1975) · The Enzymes · doi:10.1016/s1874-6047(08)60213-9 · Cited by 233 · see citing works
Stewart, G. R., Mann, A. F., & Fentem, P. A. (1980) · Elsevier eBooks · doi:10.1016/b978-0-12-675405-6.50013-9 · Cited by 192 · see citing works
The Incomplete Tricarboxylic Acid Cycle in the Blue-green Alga Anabaena Variabilis
PEARCE, J., LEACH, C. K., & CARR, N. G. (1969) · Journal of General Microbiology · doi:10.1099/00221287-55-3-371 · Cited by 159 · see citing works
Role of NAD-linked glutamate dehydrogenase in nitrogen metabolism in Saccharomyces cerevisiae
Miller, S. M., & Magasanik, B. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.9.4927-4935.1990 · Cited by 157 · see citing works
Glutamate dehydrogenase from Escherichia coli: purification and properties
Sakamoto, N., Kotre, A. M., & Savageau, M. A. (1975) · Journal of Bacteriology · doi:10.1128/jb.124.2.775-783.1975 · Cited by 119 · see citing works
Glutamate dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus
Consalvi, V., Chiaraluce, R., Politi, L., et al. (1991) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1991.tb15837.x · Cited by 73 · see citing works
Hemmilä, I. A., & Mäntsälä, P. (1978) · Biochemical Journal · doi:10.1042/bj1730045 · Cited by 63 · see citing works
Ammonia assimilation in blue-green algae
Neilson, A. H., & Doudoroff, M. (1973) · Archives of Microbiology · doi:10.1007/bf00409395 · Cited by 60 · see citing works
The crystal structure of glutamate dehydrogenase from Clostridium symbiosum at 0.6 nm resolution
Rice, D. W., Baker, P. J., Farrants, G. W., et al. (1987) · Biochemical Journal · doi:10.1042/bj2420789 · Cited by 57 · see citing works
Bonete, M.-J., Camacho, M. L., & Cadenas, E. (1986) · International Journal of Biochemistry · doi:10.1016/0020-711x(86)90054-6 · Cited by 37 · see citing works
Florencio, F. J., Marqués, S., & Candau, P. (1987) · FEBS Letters · doi:10.1016/0014-5793(87)80505-7 · Cited by 36 · see citing works
Self-citationBascomb, N. F., & Schmidt, R. R. (1987) · PLANT PHYSIOLOGY · doi:10.1104/pp.83.1.75 · Cited by 36 · see citing works
Lightfoot, D. A., Baron, A. J., & Wootton, J. C. (1988) · Plant Molecular Biology · doi:10.1007/bf00027390 · Cited by 23 · see citing works
Molin, W. T., Cunningham, T. P., Bascomb, N. F., et al. (1981) · PLANT PHYSIOLOGY · doi:10.1104/pp.67.6.1250 · Cited by 22 · see citing works
Kenzie, E. A. M., Copeland, L., & Lees, E. M. (1981) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(81)90369-6 · Cited by 11 · see citing works
The presence of glutamate dehydrogenase in Chlorogloeopsis fritschii
Sallal, A.-K. J., & Nimer, N. A. (1990) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1990.tb13866.x · Cited by 5 · see citing works
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Research works printed but not linked: 3
- Stewart. G.R., Mann. A.F. and Fentem, P.A. (1980) in: The Biochemistry of Plants (Stumpt', K. and Corm, E.E. eds) vol. 5, pp. 271-327, Academic Press, New York, London.
- Smith, E.L., Austen, B.M., Blumenthal, K.M. and Nyc, J.F. (1975) in: The Enzymes (Boyer. P.D. ed.) vol. 1 la, pp. 293-367, Academic Press, New York, San Francisco.
- WI Florencio, F.J., ChBvez, S., Muro-Pastor. M.I., Reyes, J.C., Marques, S., MCrida, A. and Candau, P. (1991) in: Trends in Photosynthesis Research (Barber, J., Guerrero, M.G. and Medrano, H. eds) Intercept Ltd., England, in press.
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Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering
Quintana, N., Van der Kooy, F., Van de Rhee, M. D., Voshol, G. P., & Verpoorte, R. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3394-0 · Cited by 338 · see citing works
- Authors
- Chávez, S., & Candau, P.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Corresponding author
- Candau, P.
- ORCID
- Chávez, S.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1991-07-08
- Volume
- 285
- Issue
- 1
- Pages
- 35-38
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1980657876
- Cited by
- 36
- References
- 19
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 1 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 1.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Keywords
- Synechocystis sp. PCC 6803, cyanobacteria, coenzyme specificity, enzyme kinetics, ammonium, alpha-ketoglutarate, NADH, hexameric enzyme, glutamate synthesis, enzyme activation
- MeSH terms
- Cyanobacteria, Ammonia, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Glutamate Dehydrogenase, Hydrogen-Ion Concentration, Isoelectric Point, Isoenzymes, Kinetics, NAD
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