The Incomplete Tricarboxylic Acid Cycle in the Blue-green Alga Anabaena Variabilis
Abstract
Summary: The presence of an incomplete tricarboxylic acid cycle in Anabaena varia-bilis and Anacystis nidulam is described. These blue-green algae lack both α-oxoglutarate dehydrogenase and succinyl-CoA synthetase. Succinyl-CoA was formed in extracts of A. variabilis by 3-ketoacyl CoA-transferase using acetoacetyl-CoA as CoA donor. The activities of the other tricarboxylic cycle enzymes were measured in extracts prepared from autotrophic organisms and from those grown in the presence of acetate. No alterations in activity indicative of enzyme repression or de-repression were observed. These results are discussed in relation to possible reasons for autotrophic behaviour.
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Cyanobacteria possess an incomplete TCA cycle in which 2-oxoglutarate dehydrogenase and succinyl-CoA synthetase are missing.
“As stated above, cyanobacteria possess an incomplete TCA cycle where two key enzymes are missing, the 2-oxoglutarate dehydrogenase (ogdh, EC 1.2.4.2), involved in the transformation of 2-OG into succinyl-CoA, and the succinyl-CoA synthetase (sucL, EC 6.2.1.4, 6.2.1.5), for the formation of succinate (Pearce et al. 1969; Stanier and Cohenbazire 1977).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Pearce et al. 1969
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- Authors
- PEARCE, J., LEACH, C. K., & CARR, N. G.
- Affiliations
- University of Liverpool (GB)
- Venue
- Journal of General Microbiology
- Publisher
- Microbiology Society
- Published
- 1969-03-01
- Volume
- 55
- Issue
- 3
- Pages
- 371-378
- ISSN
- 0022-1287 (ISSN-L)
- OpenAlex
- W2129315727
- Cited by
- 159
- References
- 9
- Citations by year
- 2025: 1 · 2023: 2 · 2022: 2 · 2020: 4 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 4 · 2015: 6 · 2014: 6 · 2013: 7 · 2012: 1
- FWCI
- 9.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of General Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- Anabaena variabilis, Anacystis nidulans, cyanobacteria, succinyl-CoA synthetase, acetoacetyl-CoA, autotrophic growth, acetate, enzyme repression
- MeSH terms
- Acetates, Biological Assay, Carbon Isotopes, Citric Acid Cycle, Coenzyme A, Culture Media, Depression, Chemical, Ligases, Oxidoreductases, Transferases, Eukaryota
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